Quantitative phase study of the dynamic cellular response in femtosecond laser photoporation

Maciej Antkowiak, Maria Leilani Torres-Mapa, Kishan Dholakia, Frank J. Gunn-Moore

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

We use Digital Holographic Microscopy to study dynamic responses of live cells to femtosecond laser cellular membrane photoporation. Temporal and spatial characteristics of morphological changes as well as dry mass variation are analyzed and compared with conventional fluorescent assays for viability and photoporation efficiency. With the latter, the results provide a new insight into the efficiency and toxicity of this novel optical method of drug delivery. In addition, quantitative phase maps reveal photoporation related sub-cellular dynamics of cytoplasmic vesicles.

Original languageEnglish
Pages (from-to)414-424
Number of pages11
JournalBiomedical Optics Express
Volume1
Issue number2
DOIs
Publication statusPublished - 2010 Sep 1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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