Quantitative profiling of phosphatidylcholine and phosphatidylethanolamine in a steatosis/fibrosis model of rat liver by nanoflow liquid chromatography/tandem mass spectrometry

Eun Jeong Ahn; Hanna Kim; Bong Chul Chung; Gu Kong; Myeong Hee Moon

doi:10.1016/j.chroma.2008.04.031

Quantitative profiling of phosphatidylcholine and phosphatidylethanolamine in a steatosis/fibrosis model of rat liver by nanoflow liquid chromatography/tandem mass spectrometry

Eun Jeong Ahn, Hanna Kim, Bong Chul Chung, Gu Kong, Myeong Hee Moon

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Quantitative analysis of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) was carried out using a steatosis/fibrosis model of rat livers, which were induced by a chronic administration of carbon tetrachloride (CCl₄). Intact phospholipid mixtures from each liver sample (from rats fed with 0.5 mL CCl₄/kg three times a week for 30, 60, and 90 days) were analyzed by nanoflow liquid chromatography-electrospray ionization tandem mass spectrometry (nLC/ESI/MS/MS), and identifications of 37 PC and 19 PE species were made by collision-induced dissociation. The quantitative analysis utilized a multiple standard addition method with an internal standard, and the relationship between the MS peak intensities of different PC species and their carbon chain length was included for calibration. It was found that the total amount of PC and PE species decreased significantly with administration of CCl₄. While concentrations of most PC and PE species decreased to a great extent, three PEs and seven PCs were up-regulated more than twofold.

Original language	English
Pages (from-to)	96-102
Number of pages	7
Journal	Journal of Chromatography A
Volume	1194
Issue number	1
DOIs	https://doi.org/10.1016/j.chroma.2008.04.031
Publication status	Published - 2008 Jun 13

Bibliographical note

Funding Information:
This study was supported by a grant (2007-02419) from the Korea Ministry of Science & Technology (MOST) and partly by a grant from the Korea Science and Engineering Foundation through the Center for Bioactive Molecular Hybrids (CBMH).

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/j.chroma.2008.04.031

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title = "Quantitative profiling of phosphatidylcholine and phosphatidylethanolamine in a steatosis/fibrosis model of rat liver by nanoflow liquid chromatography/tandem mass spectrometry",

abstract = "Quantitative analysis of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) was carried out using a steatosis/fibrosis model of rat livers, which were induced by a chronic administration of carbon tetrachloride (CCl4). Intact phospholipid mixtures from each liver sample (from rats fed with 0.5 mL CCl4/kg three times a week for 30, 60, and 90 days) were analyzed by nanoflow liquid chromatography-electrospray ionization tandem mass spectrometry (nLC/ESI/MS/MS), and identifications of 37 PC and 19 PE species were made by collision-induced dissociation. The quantitative analysis utilized a multiple standard addition method with an internal standard, and the relationship between the MS peak intensities of different PC species and their carbon chain length was included for calibration. It was found that the total amount of PC and PE species decreased significantly with administration of CCl4. While concentrations of most PC and PE species decreased to a great extent, three PEs and seven PCs were up-regulated more than twofold.",

author = "Ahn, {Eun Jeong} and Hanna Kim and Chung, {Bong Chul} and Gu Kong and Moon, {Myeong Hee}",

note = "Funding Information: This study was supported by a grant (2007-02419) from the Korea Ministry of Science & Technology (MOST) and partly by a grant from the Korea Science and Engineering Foundation through the Center for Bioactive Molecular Hybrids (CBMH).",

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Quantitative profiling of phosphatidylcholine and phosphatidylethanolamine in a steatosis/fibrosis model of rat liver by nanoflow liquid chromatography/tandem mass spectrometry. / Ahn, Eun Jeong; Kim, Hanna; Chung, Bong Chul et al.

In: Journal of Chromatography A, Vol. 1194, No. 1, 13.06.2008, p. 96-102.

Research output: Contribution to journal › Article › peer-review

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AU - Kong, Gu

AU - Moon, Myeong Hee

N1 - Funding Information: This study was supported by a grant (2007-02419) from the Korea Ministry of Science & Technology (MOST) and partly by a grant from the Korea Science and Engineering Foundation through the Center for Bioactive Molecular Hybrids (CBMH).

PY - 2008/6/13

Y1 - 2008/6/13

N2 - Quantitative analysis of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) was carried out using a steatosis/fibrosis model of rat livers, which were induced by a chronic administration of carbon tetrachloride (CCl4). Intact phospholipid mixtures from each liver sample (from rats fed with 0.5 mL CCl4/kg three times a week for 30, 60, and 90 days) were analyzed by nanoflow liquid chromatography-electrospray ionization tandem mass spectrometry (nLC/ESI/MS/MS), and identifications of 37 PC and 19 PE species were made by collision-induced dissociation. The quantitative analysis utilized a multiple standard addition method with an internal standard, and the relationship between the MS peak intensities of different PC species and their carbon chain length was included for calibration. It was found that the total amount of PC and PE species decreased significantly with administration of CCl4. While concentrations of most PC and PE species decreased to a great extent, three PEs and seven PCs were up-regulated more than twofold.

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Quantitative profiling of phosphatidylcholine and phosphatidylethanolamine in a steatosis/fibrosis model of rat liver by nanoflow liquid chromatography/tandem mass spectrometry

Abstract

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