Quantitative proteomic analyses reveal that GPX4 downregulation during myocardial infarction contributes to ferroptosis in cardiomyocytes

Tae Jun Park, Jei Hyoung Park, Ga Seul Lee, Ji Yoon Lee, Ji Hye Shin, Min Wook Kim, Yong Sook Kim, Jeong Yoon Kim, Kyoung Jin Oh, Baek Soo Han, Won Kon Kim, Youngkeun Ahn, Jeong Hee Moon, Jaewhan Song, Kwang Hee Bae, Do Han Kim, Eun Woo Lee, Sang Chul Lee

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5 Citations (Scopus)

Abstract

Ischaemic heart disease (IHD) is the leading cause of death worldwide. Although myocardial cell death plays a significant role in myocardial infarction (MI), its underlying mechanism remains to be elucidated. To understand the progression of MI and identify potential therapeutic targets, we performed tandem mass tag (TMT)-based quantitative proteomic analysis using an MI mouse model. Gene ontology (GO) analysis and gene set enrichment analysis (GSEA) revealed that the glutathione metabolic pathway and reactive oxygen species (ROS) pathway were significantly downregulated during MI. In particular, glutathione peroxidase 4 (GPX4), which protects cells from ferroptosis (an iron-dependent programme of regulated necrosis), was downregulated in the early and middle stages of MI. RNA-seq and qRT-PCR analyses suggested that GPX4 downregulation occurred at the transcriptional level. Depletion or inhibition of GPX4 using specific siRNA or the chemical inhibitor RSL3, respectively, resulted in the accumulation of lipid peroxide, leading to cell death by ferroptosis in H9c2 cardiomyoblasts. Although neonatal rat ventricular myocytes (NRVMs) were less sensitive to GPX4 inhibition than H9c2 cells, NRVMs rapidly underwent ferroptosis in response to GPX4 inhibition under cysteine deprivation. Our study suggests that downregulation of GPX4 during MI contributes to ferroptotic cell death in cardiomyocytes upon metabolic stress such as cysteine deprivation.

Original languageEnglish
Article number835
JournalCell Death and Disease
Volume10
Issue number11
DOIs
Publication statusPublished - 2019 Nov 1

All Science Journal Classification (ASJC) codes

  • Immunology
  • Cellular and Molecular Neuroscience
  • Cell Biology
  • Cancer Research

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    Park, T. J., Park, J. H., Lee, G. S., Lee, J. Y., Shin, J. H., Kim, M. W., Kim, Y. S., Kim, J. Y., Oh, K. J., Han, B. S., Kim, W. K., Ahn, Y., Moon, J. H., Song, J., Bae, K. H., Kim, D. H., Lee, E. W., & Lee, S. C. (2019). Quantitative proteomic analyses reveal that GPX4 downregulation during myocardial infarction contributes to ferroptosis in cardiomyocytes. Cell Death and Disease, 10(11), [835]. https://doi.org/10.1038/s41419-019-2061-8