Quantitative proteomic analysis of human embryonic stem cell differentiation by 8-Plex iTRAQ labelling

Mahdieh Jadaliha, Hyoung Joo Lee, Mohammad Pakzad, Ali Fathi, Seul Ki Jeong, Sang Yun Cho, Hossein Baharvand, Young Ki Paik, Ghasem Hosseini Salekdeh

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Analysis of gene expression to define molecular mechanisms and pathways involved in human embryonic stem cells (hESCs) proliferation and differentiations has allowed for further deciphering of the self-renewal and pluripotency characteristics of hESC. Proteins associated with hESCs were discovered through isobaric tags for relative and absolute quantification (iTRAQ). Undifferentiated hESCs and hESCs in different stages of spontaneous differentiation by embryoid body (EB) formation were analyzed. Using the iTRAQ approach, we identified 156 differentially expressed proteins involved in cell proliferation, apoptosis, transcription, translation, mRNA processing, and protein synthesis. Proteins involved in nucleic acid binding, protein synthesis, and integrin signaling were downregulated during differentiation, whereas cytoskeleton proteins were upregulated. The present findings added insight to our understanding of the mechanisms involved in hESC proliferation and differentiation.

Original languageEnglish
Article numbere38532
JournalPloS one
Volume7
Issue number6
DOIs
Publication statusPublished - 2012 Jun 18

Fingerprint

embryonic stem cells
Stem cells
cell differentiation
Proteomics
proteomics
Labeling
Cell Differentiation
Cell proliferation
cell proliferation
Cell Proliferation
Protein Biosynthesis
Proteins
proteins
protein synthesis
Embryoid Bodies
integrins
Transcription
cytoskeleton
Cytoskeleton
Gene expression

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Jadaliha, M., Lee, H. J., Pakzad, M., Fathi, A., Jeong, S. K., Cho, S. Y., ... Salekdeh, G. H. (2012). Quantitative proteomic analysis of human embryonic stem cell differentiation by 8-Plex iTRAQ labelling. PloS one, 7(6), [e38532]. https://doi.org/10.1371/journal.pone.0038532
Jadaliha, Mahdieh ; Lee, Hyoung Joo ; Pakzad, Mohammad ; Fathi, Ali ; Jeong, Seul Ki ; Cho, Sang Yun ; Baharvand, Hossein ; Paik, Young Ki ; Salekdeh, Ghasem Hosseini. / Quantitative proteomic analysis of human embryonic stem cell differentiation by 8-Plex iTRAQ labelling. In: PloS one. 2012 ; Vol. 7, No. 6.
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Jadaliha, M, Lee, HJ, Pakzad, M, Fathi, A, Jeong, SK, Cho, SY, Baharvand, H, Paik, YK & Salekdeh, GH 2012, 'Quantitative proteomic analysis of human embryonic stem cell differentiation by 8-Plex iTRAQ labelling', PloS one, vol. 7, no. 6, e38532. https://doi.org/10.1371/journal.pone.0038532

Quantitative proteomic analysis of human embryonic stem cell differentiation by 8-Plex iTRAQ labelling. / Jadaliha, Mahdieh; Lee, Hyoung Joo; Pakzad, Mohammad; Fathi, Ali; Jeong, Seul Ki; Cho, Sang Yun; Baharvand, Hossein; Paik, Young Ki; Salekdeh, Ghasem Hosseini.

In: PloS one, Vol. 7, No. 6, e38532, 18.06.2012.

Research output: Contribution to journalArticle

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AU - Cho, Sang Yun

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