Quantitative proteomic analysis of human embryonic stem cell differentiation by 8-Plex iTRAQ labelling

Mahdieh Jadaliha, Hyoung Joo Lee, Mohammad Pakzad, Ali Fathi, Seul Ki Jeong, Sang Yun Cho, Hossein Baharvand, Young Ki Paik, Ghasem Hosseini Salekdeh

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19 Citations (Scopus)

Abstract

Analysis of gene expression to define molecular mechanisms and pathways involved in human embryonic stem cells (hESCs) proliferation and differentiations has allowed for further deciphering of the self-renewal and pluripotency characteristics of hESC. Proteins associated with hESCs were discovered through isobaric tags for relative and absolute quantification (iTRAQ). Undifferentiated hESCs and hESCs in different stages of spontaneous differentiation by embryoid body (EB) formation were analyzed. Using the iTRAQ approach, we identified 156 differentially expressed proteins involved in cell proliferation, apoptosis, transcription, translation, mRNA processing, and protein synthesis. Proteins involved in nucleic acid binding, protein synthesis, and integrin signaling were downregulated during differentiation, whereas cytoskeleton proteins were upregulated. The present findings added insight to our understanding of the mechanisms involved in hESC proliferation and differentiation.

Original languageEnglish
Article numbere38532
JournalPloS one
Volume7
Issue number6
DOIs
Publication statusPublished - 2012 Jun 18

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Jadaliha, M., Lee, H. J., Pakzad, M., Fathi, A., Jeong, S. K., Cho, S. Y., Baharvand, H., Paik, Y. K., & Salekdeh, G. H. (2012). Quantitative proteomic analysis of human embryonic stem cell differentiation by 8-Plex iTRAQ labelling. PloS one, 7(6), [e38532]. https://doi.org/10.1371/journal.pone.0038532