Quercetin protects obesity-induced hypothalamic inflammation by reducing microglia-mediated inflammatory responses via HO-1 induction

Jihyeon Yang, Chu Sook Kim, Thai Hien Tu, Min Seon Kim, Tsuyoshi Goto, Teruo Kawada, Myung Sook Choi, Taesun Park, Mi Kyung Sung, Jong Won Yun, Suck Young Choe, Jee Hye Lee, Yeonsoo Joe, Hye Seon Choi, Sung Hoon Back, Hun Taeg Chung, Rina Yu

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Obesity-induced hypothalamic inflammation is characterized by activation of microglia, which are resident macrophages of the central nervous system, and is implicated in the derangement of energy homeostasis, metabolic complications, and neurodegenerative diseases. Quercetin, a naturally occurring flavonoid, is known to protect against oxidative stress and inflammation-related metabolic complications. Here, we demonstrate that quercetin reduces obesity-induced hypothalamic inflammation by inhibiting microglia-mediated inflammatory responses, and the beneficial action of quercetin is associated with heme oxygenase (HO-1) induction. Quercetin markedly reduced the production of inflammatory mediators (monocyte chemoattractant protein (MCP)-1, interleukin (IL-6), IL-1β, nitric oxide) by microglia stimulated with saturated fatty acid palmitate and/or lipid-laden microglia-conditioned medium. Quercetin also upregulated the expression of HO-1 in palmitate-treated lipid-laden microglia, and the actions of quercetin against microglia activation accompanied by IκBα degradation were abolished by a HO-1 inhibitor. Moreover, quercetin supplementation reduced the levels of inflammatory cytokines and microglia activation markers in the hypothalamus of high fat diet (HFD)-fed obese mice, which was accompanied by upregulation of HO-1. These findings indicate that quercetin suppresses microglia-mediated inflammatory responses via the induction of HO-1, and hence protects against obesity-induced hypothalamic inflammation.

Original languageEnglish
Article number650
JournalNutrients
Volume9
Issue number7
DOIs
Publication statusPublished - 2017 Jul

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Quercetin
neuroglia
Microglia
quercetin
obesity
Obesity
inflammation
Inflammation
Palmitates
palmitates
heme oxygenase (biliverdin-producing)
Interleukin-6
Lipids
Obese Mice
Heme Oxygenase-1
Chemokine CCL2
complications (disease)
Metabolic Diseases
interleukin-1
neurodegenerative diseases

All Science Journal Classification (ASJC) codes

  • Food Science
  • Nutrition and Dietetics

Cite this

Yang, Jihyeon ; Kim, Chu Sook ; Tu, Thai Hien ; Kim, Min Seon ; Goto, Tsuyoshi ; Kawada, Teruo ; Choi, Myung Sook ; Park, Taesun ; Sung, Mi Kyung ; Yun, Jong Won ; Choe, Suck Young ; Lee, Jee Hye ; Joe, Yeonsoo ; Choi, Hye Seon ; Back, Sung Hoon ; Chung, Hun Taeg ; Yu, Rina. / Quercetin protects obesity-induced hypothalamic inflammation by reducing microglia-mediated inflammatory responses via HO-1 induction. In: Nutrients. 2017 ; Vol. 9, No. 7.
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title = "Quercetin protects obesity-induced hypothalamic inflammation by reducing microglia-mediated inflammatory responses via HO-1 induction",
abstract = "Obesity-induced hypothalamic inflammation is characterized by activation of microglia, which are resident macrophages of the central nervous system, and is implicated in the derangement of energy homeostasis, metabolic complications, and neurodegenerative diseases. Quercetin, a naturally occurring flavonoid, is known to protect against oxidative stress and inflammation-related metabolic complications. Here, we demonstrate that quercetin reduces obesity-induced hypothalamic inflammation by inhibiting microglia-mediated inflammatory responses, and the beneficial action of quercetin is associated with heme oxygenase (HO-1) induction. Quercetin markedly reduced the production of inflammatory mediators (monocyte chemoattractant protein (MCP)-1, interleukin (IL-6), IL-1β, nitric oxide) by microglia stimulated with saturated fatty acid palmitate and/or lipid-laden microglia-conditioned medium. Quercetin also upregulated the expression of HO-1 in palmitate-treated lipid-laden microglia, and the actions of quercetin against microglia activation accompanied by IκBα degradation were abolished by a HO-1 inhibitor. Moreover, quercetin supplementation reduced the levels of inflammatory cytokines and microglia activation markers in the hypothalamus of high fat diet (HFD)-fed obese mice, which was accompanied by upregulation of HO-1. These findings indicate that quercetin suppresses microglia-mediated inflammatory responses via the induction of HO-1, and hence protects against obesity-induced hypothalamic inflammation.",
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Yang, J, Kim, CS, Tu, TH, Kim, MS, Goto, T, Kawada, T, Choi, MS, Park, T, Sung, MK, Yun, JW, Choe, SY, Lee, JH, Joe, Y, Choi, HS, Back, SH, Chung, HT & Yu, R 2017, 'Quercetin protects obesity-induced hypothalamic inflammation by reducing microglia-mediated inflammatory responses via HO-1 induction', Nutrients, vol. 9, no. 7, 650. https://doi.org/10.3390/nu9070650

Quercetin protects obesity-induced hypothalamic inflammation by reducing microglia-mediated inflammatory responses via HO-1 induction. / Yang, Jihyeon; Kim, Chu Sook; Tu, Thai Hien; Kim, Min Seon; Goto, Tsuyoshi; Kawada, Teruo; Choi, Myung Sook; Park, Taesun; Sung, Mi Kyung; Yun, Jong Won; Choe, Suck Young; Lee, Jee Hye; Joe, Yeonsoo; Choi, Hye Seon; Back, Sung Hoon; Chung, Hun Taeg; Yu, Rina.

In: Nutrients, Vol. 9, No. 7, 650, 07.2017.

Research output: Contribution to journalArticle

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T1 - Quercetin protects obesity-induced hypothalamic inflammation by reducing microglia-mediated inflammatory responses via HO-1 induction

AU - Yang, Jihyeon

AU - Kim, Chu Sook

AU - Tu, Thai Hien

AU - Kim, Min Seon

AU - Goto, Tsuyoshi

AU - Kawada, Teruo

AU - Choi, Myung Sook

AU - Park, Taesun

AU - Sung, Mi Kyung

AU - Yun, Jong Won

AU - Choe, Suck Young

AU - Lee, Jee Hye

AU - Joe, Yeonsoo

AU - Choi, Hye Seon

AU - Back, Sung Hoon

AU - Chung, Hun Taeg

AU - Yu, Rina

PY - 2017/7

Y1 - 2017/7

N2 - Obesity-induced hypothalamic inflammation is characterized by activation of microglia, which are resident macrophages of the central nervous system, and is implicated in the derangement of energy homeostasis, metabolic complications, and neurodegenerative diseases. Quercetin, a naturally occurring flavonoid, is known to protect against oxidative stress and inflammation-related metabolic complications. Here, we demonstrate that quercetin reduces obesity-induced hypothalamic inflammation by inhibiting microglia-mediated inflammatory responses, and the beneficial action of quercetin is associated with heme oxygenase (HO-1) induction. Quercetin markedly reduced the production of inflammatory mediators (monocyte chemoattractant protein (MCP)-1, interleukin (IL-6), IL-1β, nitric oxide) by microglia stimulated with saturated fatty acid palmitate and/or lipid-laden microglia-conditioned medium. Quercetin also upregulated the expression of HO-1 in palmitate-treated lipid-laden microglia, and the actions of quercetin against microglia activation accompanied by IκBα degradation were abolished by a HO-1 inhibitor. Moreover, quercetin supplementation reduced the levels of inflammatory cytokines and microglia activation markers in the hypothalamus of high fat diet (HFD)-fed obese mice, which was accompanied by upregulation of HO-1. These findings indicate that quercetin suppresses microglia-mediated inflammatory responses via the induction of HO-1, and hence protects against obesity-induced hypothalamic inflammation.

AB - Obesity-induced hypothalamic inflammation is characterized by activation of microglia, which are resident macrophages of the central nervous system, and is implicated in the derangement of energy homeostasis, metabolic complications, and neurodegenerative diseases. Quercetin, a naturally occurring flavonoid, is known to protect against oxidative stress and inflammation-related metabolic complications. Here, we demonstrate that quercetin reduces obesity-induced hypothalamic inflammation by inhibiting microglia-mediated inflammatory responses, and the beneficial action of quercetin is associated with heme oxygenase (HO-1) induction. Quercetin markedly reduced the production of inflammatory mediators (monocyte chemoattractant protein (MCP)-1, interleukin (IL-6), IL-1β, nitric oxide) by microglia stimulated with saturated fatty acid palmitate and/or lipid-laden microglia-conditioned medium. Quercetin also upregulated the expression of HO-1 in palmitate-treated lipid-laden microglia, and the actions of quercetin against microglia activation accompanied by IκBα degradation were abolished by a HO-1 inhibitor. Moreover, quercetin supplementation reduced the levels of inflammatory cytokines and microglia activation markers in the hypothalamus of high fat diet (HFD)-fed obese mice, which was accompanied by upregulation of HO-1. These findings indicate that quercetin suppresses microglia-mediated inflammatory responses via the induction of HO-1, and hence protects against obesity-induced hypothalamic inflammation.

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