R-(-)-β-O-methylsynephrine, a natural product, inhibits VEGF-induced angiogenesis in vitro and in vivo

Nam Hee Kim, Ngoc Bich Pham, Ronald J. Quinn, Ho Jeong Kwon

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

R-(-)-β-O-methylsynephrine (OMe-Syn) is an active compound isolated from a plant of the Rutaceae family. We conducted cell proliferation assays on various cell lines and found that OMe-Syn more strongly inhibited the growth of human umbilical vein endothelial cells (HUVECs) than that of other normal and cancer cell lines tested. In angiogenesis assays, it inhibited vascular endothelial growth factor (VEGF)-induced invasion and tube formation of HUVECs with no toxicity. The anti-angiogenic activity of OMe-Syn was also validated in vivo using the chorioallantonic membrane (CAM) assay in growing chick embryos. Expression of the growth factors VEGF, hepatocyte growth factor, and basic fibroblast growth factor was suppressed by OMe-Syn in a dose-dependent manner. Taken together, our results indicate that this compound could be a novel basis for a small molecule targeting angiogenesis.

Original languageEnglish
Pages (from-to)20-23
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume399
Issue number1
DOIs
Publication statusPublished - 2010 Aug 1

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Biological Products
Vascular Endothelial Growth Factor A
Assays
Endothelial cells
Human Umbilical Vein Endothelial Cells
Cells
Rutaceae
Cell Line
Hepatocyte Growth Factor
Cell proliferation
Fibroblast Growth Factor 2
Chick Embryo
Toxicity
Intercellular Signaling Peptides and Proteins
Cell Proliferation
Membranes
Molecules
methylsynephrine
In Vitro Techniques
Growth

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "R-(-)-β-O-methylsynephrine (OMe-Syn) is an active compound isolated from a plant of the Rutaceae family. We conducted cell proliferation assays on various cell lines and found that OMe-Syn more strongly inhibited the growth of human umbilical vein endothelial cells (HUVECs) than that of other normal and cancer cell lines tested. In angiogenesis assays, it inhibited vascular endothelial growth factor (VEGF)-induced invasion and tube formation of HUVECs with no toxicity. The anti-angiogenic activity of OMe-Syn was also validated in vivo using the chorioallantonic membrane (CAM) assay in growing chick embryos. Expression of the growth factors VEGF, hepatocyte growth factor, and basic fibroblast growth factor was suppressed by OMe-Syn in a dose-dependent manner. Taken together, our results indicate that this compound could be a novel basis for a small molecule targeting angiogenesis.",
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R-(-)-β-O-methylsynephrine, a natural product, inhibits VEGF-induced angiogenesis in vitro and in vivo. / Kim, Nam Hee; Pham, Ngoc Bich; Quinn, Ronald J.; Kwon, Ho Jeong.

In: Biochemical and Biophysical Research Communications, Vol. 399, No. 1, 01.08.2010, p. 20-23.

Research output: Contribution to journalArticle

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