Radiation-induced alteration of pain-related signals in an animal model with bone invasion from cancer

Jinsil Seong, Hee Chul Park, Jiyoung Kim, Un Jung Kim, Bae Whan Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Although radiotherapy is highly effective in relieving bone pain from cancer invasion, the mechanism of pain relief remains unclear. To explore the mechanism of radiotherapy-induced analgesia, we have developed an animal model of bone pain resulting from cancer invasion. Using this animal model system, radiation-induced pain response and pain-related signals in the spinal cord were analyzed. The hind paw model of bone pain from cancer invasion was developed by injecting transplantable hepatocellular carcinoma, IICa-1, into the periosteal membrane of the foot dorsum in C3H/HeJ mice. Bony invasion from HCa-1 cells was confirmed by histopathological examinations. We also measured the development of pain-associated behaviors. In this model, changes in the objective level of pain response after irradiation of the tumor were analyzed. Expression of pain-related host signals in the spinal cord, such as calcitonin gene-related peptide (CGRP), substance P, and c-fos, was investigated with immunohistochemical staining. In the histopathological examinations, bone invasion from HCa-I cells was seen from day 7 and was evident at day 14 after injection. Measurable pain-associated behaviors were developed from day 7. In this model, mice treated with radiotherapy showed decreased objective levels of pain with a higher threshold to graded mechanical stimulation than did control mice from day 3 after irradiation. After irradiation of tumors, significant decreases in the expression of CGRP were shown in the spinal cord, whereas neither substance P nor c-fos showed any alteration. We developed a novel hind paw model of bone pain from cancer invasion that was confirmed by histopathological examination and measurable pain-associated behaviors. Radiotherapy decreased the objective level of pain and the underlying mechanism involved in the alteration of pain-related host signal, CGRP, in the spinal cord.

Original languageEnglish
Pages (from-to)179-186
Number of pages8
JournalAnnals of the New York Academy of Sciences
Volume1030
DOIs
Publication statusPublished - 2004 Jan 1

Fingerprint

Bone Neoplasms
Bone
Animals
Animal Models
Radiotherapy
Radiation
Pain
Calcitonin Gene-Related Peptide
Irradiation
Substance P
Tumors
Spinal Cord
Animal Model
Cancer
Invasion
Alteration
Membranes
Bone and Bones
Inbred C3H Mouse
Analgesia

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Seong, Jinsil ; Park, Hee Chul ; Kim, Jiyoung ; Kim, Un Jung ; Lee, Bae Whan. / Radiation-induced alteration of pain-related signals in an animal model with bone invasion from cancer. In: Annals of the New York Academy of Sciences. 2004 ; Vol. 1030. pp. 179-186.
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Radiation-induced alteration of pain-related signals in an animal model with bone invasion from cancer. / Seong, Jinsil; Park, Hee Chul; Kim, Jiyoung; Kim, Un Jung; Lee, Bae Whan.

In: Annals of the New York Academy of Sciences, Vol. 1030, 01.01.2004, p. 179-186.

Research output: Contribution to journalArticle

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