RAGE ligands induce apoptotic cell death of pancreatic β-cells via oxidative stress

Byung Wan Lee, Hee Young Chae, Soo Jin Kwon, So Youn Park, Jahei Ihm, Sung Hee Ihm

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Activation of the receptor for advanced glycation endproducts (RAGE) by its ligands leads to cellular damage contributing to diabetic complications. It is not clearly known whether RAGE ligands influence pancreatic β-cells. In this study, we investigated the expression of RAGE in islet cells and the effect of RAGE ligands, S100b and HMG-1, on islet cells. RAGE was expressed in INS-1 cells and isolated rat and human islets at mRNA and protein levels. RAGE and its ligand, S100b, were detected on islet cells in 28-week-old diabetic OLETF rats. Both S100b and HMG-1 induced apoptotic cell death of INS-1 and islet cells. This INS-1 cell apoptosis was accompanied by increased intracellular oxidative stress and inhibited by antioxidants or a NADPH oxidase inhibitor. Our results showing S100b/RAGE expression on islets of diabetic rat model and RAGE ligands-induced islet cell apoptosis via NADPH oxidase-mediated ROS generation suggest that RAGE ligands-RAGE interaction may contribute not only to the development of diabetic complications but also to the progressive β-cell loss in type 2 diabetes by inducing oxidative stress.

Original languageEnglish
Pages (from-to)813-818
Number of pages6
JournalInternational journal of molecular medicine
Volume26
Issue number6
DOIs
Publication statusPublished - 2010 Dec 1

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Oxidative Stress
Cell Death
Ligands
Islets of Langerhans
NADPH Oxidase
Diabetes Complications
Inbred OLETF Rats
Advanced Glycosylation End Product-Specific Receptor
Apoptosis
Type 2 Diabetes Mellitus
Antioxidants
Messenger RNA
Proteins

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Lee, Byung Wan ; Chae, Hee Young ; Kwon, Soo Jin ; Park, So Youn ; Ihm, Jahei ; Ihm, Sung Hee. / RAGE ligands induce apoptotic cell death of pancreatic β-cells via oxidative stress. In: International journal of molecular medicine. 2010 ; Vol. 26, No. 6. pp. 813-818.
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RAGE ligands induce apoptotic cell death of pancreatic β-cells via oxidative stress. / Lee, Byung Wan; Chae, Hee Young; Kwon, Soo Jin; Park, So Youn; Ihm, Jahei; Ihm, Sung Hee.

In: International journal of molecular medicine, Vol. 26, No. 6, 01.12.2010, p. 813-818.

Research output: Contribution to journalArticle

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