RAGE ligands induce apoptotic cell death of pancreatic β-cells via oxidative stress

Byung Wan Lee, Hee Young Chae, Soo Jin Kwon, So Youn Park, Jahei Ihm, Sung Hee Ihm

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Activation of the receptor for advanced glycation endproducts (RAGE) by its ligands leads to cellular damage contributing to diabetic complications. It is not clearly known whether RAGE ligands influence pancreatic β-cells. In this study, we investigated the expression of RAGE in islet cells and the effect of RAGE ligands, S100b and HMG-1, on islet cells. RAGE was expressed in INS-1 cells and isolated rat and human islets at mRNA and protein levels. RAGE and its ligand, S100b, were detected on islet cells in 28-week-old diabetic OLETF rats. Both S100b and HMG-1 induced apoptotic cell death of INS-1 and islet cells. This INS-1 cell apoptosis was accompanied by increased intracellular oxidative stress and inhibited by antioxidants or a NADPH oxidase inhibitor. Our results showing S100b/RAGE expression on islets of diabetic rat model and RAGE ligands-induced islet cell apoptosis via NADPH oxidase-mediated ROS generation suggest that RAGE ligands-RAGE interaction may contribute not only to the development of diabetic complications but also to the progressive β-cell loss in type 2 diabetes by inducing oxidative stress.

Original languageEnglish
Pages (from-to)813-818
Number of pages6
JournalInternational journal of molecular medicine
Volume26
Issue number6
DOIs
Publication statusPublished - 2010 Dec

All Science Journal Classification (ASJC) codes

  • Genetics

Fingerprint Dive into the research topics of 'RAGE ligands induce apoptotic cell death of pancreatic β-cells via oxidative stress'. Together they form a unique fingerprint.

Cite this