Raman-activated cell counting for profiling carbon dioxide fixing microorganisms

Mengqiu Li, Praveen C. Ashok, Kishan Dholakia, Wei E. Huang

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Raman microspectroscopy is a label-free and nondestructive technique to measure the intrinsic chemical profile of single cells. The naturally weak Raman signals hampered the application of Raman spectroscopy for high-throughput measurements. Nearly all photosynthetic microorganisms contain carotenoids that are active molecules for resonance Raman at a 532 nm excitation wavelength. Hence, the acquisition time for a single photosynthetic microorganism can be as short as 1 ms. The carotenoid bands in Raman spectra of photosynthetic microorganisms utilizing 13CO 2 shifted when compared to the spectra of cells utilizing 12CO 2. Here, a mixture of 12C- and 13C-cyanobacterial cells were counted using a microfluidic-device-based Raman-activated cell counting procedure to prove the concept that Raman spectroscopy can be used as a high-throughput method to profile a cell population.

Original languageEnglish
Pages (from-to)6560-6563
Number of pages4
JournalJournal of Physical Chemistry A
Volume116
Issue number25
DOIs
Publication statusPublished - 2012 Jun 28

All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry

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