Raman detection of localized transferrin-coated gold nanoparticles inside a single cell

Jin Ho Park, Jen Park, Uuriintuya Dembereldorj, Keunchang Cho, Kangtaek Lee, Sung Ik Yang, So Yeong Lee, Sang Woo Joo

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We investigated the cellular uptake behavior of non-fluorescent metal nanoparticles (NPs) by use of surface-enhanced Raman scattering (SERS) combined with dark-field microscopy (DFM). The uptake of Au NPs inside a single cell could also be identified by DFM first and then confirmed by z-depth-dependent SERS at micrometer resolution. Guided by DFM for the location of Au NPs, an intracellular distribution assay was possible using Raman dyes with unique vibrational marker bands in order to identify the three-dimensional location inside the single cell by obtaining specific spectral features. Au NPs modified by 4-mercaptobenzoic acid (MBA) bearing its -COOH surface functional group were used to conjugate transferrin (Tf) protein using the 1-ethyl-3-[3- dimethylaminopropyl]carbodiimide hydrochloride (EDC) reaction. The protein conjugation reaction on Au surfaces was examined by means of color change, absorption spectroscopy, and SERS. Our results demonstrate that DFM techniques combined with SERS may have great potential for monitoring biological processes with protein conjugation at the single-cell level. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)1635-1643
Number of pages9
JournalAnalytical and Bioanalytical Chemistry
Volume401
Issue number5
DOIs
Publication statusPublished - 2011 Sep 1

Fingerprint

Raman Spectrum Analysis
Transferrin
Gold
Nanoparticles
Microscopy
Raman scattering
Microscopic examination
Ethyldimethylaminopropyl Carbodiimide
Metal Nanoparticles
Biological Phenomena
Proteins
Bearings (structural)
Spectrum Analysis
Coloring Agents
Color
Metal nanoparticles
Absorption spectroscopy
Functional groups
Assays
Monitoring

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

Cite this

Park, Jin Ho ; Park, Jen ; Dembereldorj, Uuriintuya ; Cho, Keunchang ; Lee, Kangtaek ; Yang, Sung Ik ; Lee, So Yeong ; Joo, Sang Woo. / Raman detection of localized transferrin-coated gold nanoparticles inside a single cell. In: Analytical and Bioanalytical Chemistry. 2011 ; Vol. 401, No. 5. pp. 1635-1643.
@article{405c20c22f9d462ea2124e6abc20a756,
title = "Raman detection of localized transferrin-coated gold nanoparticles inside a single cell",
abstract = "We investigated the cellular uptake behavior of non-fluorescent metal nanoparticles (NPs) by use of surface-enhanced Raman scattering (SERS) combined with dark-field microscopy (DFM). The uptake of Au NPs inside a single cell could also be identified by DFM first and then confirmed by z-depth-dependent SERS at micrometer resolution. Guided by DFM for the location of Au NPs, an intracellular distribution assay was possible using Raman dyes with unique vibrational marker bands in order to identify the three-dimensional location inside the single cell by obtaining specific spectral features. Au NPs modified by 4-mercaptobenzoic acid (MBA) bearing its -COOH surface functional group were used to conjugate transferrin (Tf) protein using the 1-ethyl-3-[3- dimethylaminopropyl]carbodiimide hydrochloride (EDC) reaction. The protein conjugation reaction on Au surfaces was examined by means of color change, absorption spectroscopy, and SERS. Our results demonstrate that DFM techniques combined with SERS may have great potential for monitoring biological processes with protein conjugation at the single-cell level. [Figure not available: see fulltext.]",
author = "Park, {Jin Ho} and Jen Park and Uuriintuya Dembereldorj and Keunchang Cho and Kangtaek Lee and Yang, {Sung Ik} and Lee, {So Yeong} and Joo, {Sang Woo}",
year = "2011",
month = "9",
day = "1",
doi = "10.1007/s00216-011-5215-9",
language = "English",
volume = "401",
pages = "1635--1643",
journal = "Fresenius Zeitschrift fur Analytische Chemie",
issn = "0016-1152",
publisher = "Springer Verlag",
number = "5",

}

Park, JH, Park, J, Dembereldorj, U, Cho, K, Lee, K, Yang, SI, Lee, SY & Joo, SW 2011, 'Raman detection of localized transferrin-coated gold nanoparticles inside a single cell', Analytical and Bioanalytical Chemistry, vol. 401, no. 5, pp. 1635-1643. https://doi.org/10.1007/s00216-011-5215-9

Raman detection of localized transferrin-coated gold nanoparticles inside a single cell. / Park, Jin Ho; Park, Jen; Dembereldorj, Uuriintuya; Cho, Keunchang; Lee, Kangtaek; Yang, Sung Ik; Lee, So Yeong; Joo, Sang Woo.

In: Analytical and Bioanalytical Chemistry, Vol. 401, No. 5, 01.09.2011, p. 1635-1643.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Raman detection of localized transferrin-coated gold nanoparticles inside a single cell

AU - Park, Jin Ho

AU - Park, Jen

AU - Dembereldorj, Uuriintuya

AU - Cho, Keunchang

AU - Lee, Kangtaek

AU - Yang, Sung Ik

AU - Lee, So Yeong

AU - Joo, Sang Woo

PY - 2011/9/1

Y1 - 2011/9/1

N2 - We investigated the cellular uptake behavior of non-fluorescent metal nanoparticles (NPs) by use of surface-enhanced Raman scattering (SERS) combined with dark-field microscopy (DFM). The uptake of Au NPs inside a single cell could also be identified by DFM first and then confirmed by z-depth-dependent SERS at micrometer resolution. Guided by DFM for the location of Au NPs, an intracellular distribution assay was possible using Raman dyes with unique vibrational marker bands in order to identify the three-dimensional location inside the single cell by obtaining specific spectral features. Au NPs modified by 4-mercaptobenzoic acid (MBA) bearing its -COOH surface functional group were used to conjugate transferrin (Tf) protein using the 1-ethyl-3-[3- dimethylaminopropyl]carbodiimide hydrochloride (EDC) reaction. The protein conjugation reaction on Au surfaces was examined by means of color change, absorption spectroscopy, and SERS. Our results demonstrate that DFM techniques combined with SERS may have great potential for monitoring biological processes with protein conjugation at the single-cell level. [Figure not available: see fulltext.]

AB - We investigated the cellular uptake behavior of non-fluorescent metal nanoparticles (NPs) by use of surface-enhanced Raman scattering (SERS) combined with dark-field microscopy (DFM). The uptake of Au NPs inside a single cell could also be identified by DFM first and then confirmed by z-depth-dependent SERS at micrometer resolution. Guided by DFM for the location of Au NPs, an intracellular distribution assay was possible using Raman dyes with unique vibrational marker bands in order to identify the three-dimensional location inside the single cell by obtaining specific spectral features. Au NPs modified by 4-mercaptobenzoic acid (MBA) bearing its -COOH surface functional group were used to conjugate transferrin (Tf) protein using the 1-ethyl-3-[3- dimethylaminopropyl]carbodiimide hydrochloride (EDC) reaction. The protein conjugation reaction on Au surfaces was examined by means of color change, absorption spectroscopy, and SERS. Our results demonstrate that DFM techniques combined with SERS may have great potential for monitoring biological processes with protein conjugation at the single-cell level. [Figure not available: see fulltext.]

UR - http://www.scopus.com/inward/record.url?scp=80052560145&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052560145&partnerID=8YFLogxK

U2 - 10.1007/s00216-011-5215-9

DO - 10.1007/s00216-011-5215-9

M3 - Article

C2 - 21744236

AN - SCOPUS:80052560145

VL - 401

SP - 1635

EP - 1643

JO - Fresenius Zeitschrift fur Analytische Chemie

JF - Fresenius Zeitschrift fur Analytische Chemie

SN - 0016-1152

IS - 5

ER -