Raman spectra of single cells with autofluorescence suppression by modulated wavelength excitation

Christoph Krafft, Sebastian Dochow, Norbert Bergner, Joachim H. Clement, Bavishna B. Praveen, Michael Mazilu, Rob Marchington, Kishan Dholakia, Jürgen Popp

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Raman spectroscopy is a non-invasive technique offering great potential in the biomedical field for label-free discrimination between normal and tumor cells based on their biochemical composition. First, this contribution describes Raman spectra of lymphocytes after drying, in laser tweezers, and trapped in a microfluidic environment. Second, spectral differences between lymphocytes and acute myeloid leukemia cells (OCI-AML3) are compared for these three experimental conditions. Significant similarities of difference spectra are consistent with the biological relevance of the spectral features. Third, modulated wavelength Raman spectroscopy has been applied to this model system to demonstrate background suppression. Here, the laser excitation wavelength of 785 nm was modulated with a frequency of 40 mHz by 0.6 nm. 40 spectra were accumulated with an exposure time of 5 seconds each. These data were subjected to principal component analysis to calculate modulated Raman signatures. The loading of the principal component shows characteristics of first derivatives with derivative like band shapes. The derivative of this loading corresponds to a pseudo-second derivative spectrum and enables to determine band positions.

Original languageEnglish
Title of host publicationBiomedical Vibrational Spectroscopy V
Subtitle of host publicationAdvances in Research and Industry
DOIs
Publication statusPublished - 2012 Jun 11
EventBiomedical Vibrational Spectroscopy V: Advances in Research and Industry - San Francisco, CA, United States
Duration: 2012 Jan 212012 Jan 22

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8219
ISSN (Print)1605-7422

Conference

ConferenceBiomedical Vibrational Spectroscopy V: Advances in Research and Industry
CountryUnited States
CitySan Francisco, CA
Period12/1/2112/1/22

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All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Krafft, C., Dochow, S., Bergner, N., Clement, J. H., Praveen, B. B., Mazilu, M., Marchington, R., Dholakia, K., & Popp, J. (2012). Raman spectra of single cells with autofluorescence suppression by modulated wavelength excitation. In Biomedical Vibrational Spectroscopy V: Advances in Research and Industry [82190F] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8219). https://doi.org/10.1117/12.908564