Abstract
Raman spectroscopy is a non-invasive technique offering great potential in the biomedical field for label-free discrimination between normal and tumor cells based on their biochemical composition. First, this contribution describes Raman spectra of lymphocytes after drying, in laser tweezers, and trapped in a microfluidic environment. Second, spectral differences between lymphocytes and acute myeloid leukemia cells (OCI-AML3) are compared for these three experimental conditions. Significant similarities of difference spectra are consistent with the biological relevance of the spectral features. Third, modulated wavelength Raman spectroscopy has been applied to this model system to demonstrate background suppression. Here, the laser excitation wavelength of 785 nm was modulated with a frequency of 40 mHz by 0.6 nm. 40 spectra were accumulated with an exposure time of 5 seconds each. These data were subjected to principal component analysis to calculate modulated Raman signatures. The loading of the principal component shows characteristics of first derivatives with derivative like band shapes. The derivative of this loading corresponds to a pseudo-second derivative spectrum and enables to determine band positions.
| Original language | English |
|---|---|
| Title of host publication | Biomedical Vibrational Spectroscopy V |
| Subtitle of host publication | Advances in Research and Industry |
| DOIs | |
| Publication status | Published - 2012 Jun 11 |
| Event | Biomedical Vibrational Spectroscopy V: Advances in Research and Industry - San Francisco, CA, United States Duration: 2012 Jan 21 → 2012 Jan 22 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 8219 |
| ISSN (Print) | 1605-7422 |
Conference
| Conference | Biomedical Vibrational Spectroscopy V: Advances in Research and Industry |
|---|---|
| Country | United States |
| City | San Francisco, CA |
| Period | 12/1/21 → 12/1/22 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging
Cite this
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Raman spectra of single cells with autofluorescence suppression by modulated wavelength excitation. / Krafft, Christoph; Dochow, Sebastian; Bergner, Norbert; Clement, Joachim H.; Praveen, Bavishna B.; Mazilu, Michael; Marchington, Rob; Dholakia, Kishan; Popp, Jürgen.
Biomedical Vibrational Spectroscopy V: Advances in Research and Industry. 2012. 82190F (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8219).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Raman spectra of single cells with autofluorescence suppression by modulated wavelength excitation
AU - Krafft, Christoph
AU - Dochow, Sebastian
AU - Bergner, Norbert
AU - Clement, Joachim H.
AU - Praveen, Bavishna B.
AU - Mazilu, Michael
AU - Marchington, Rob
AU - Dholakia, Kishan
AU - Popp, Jürgen
PY - 2012/6/11
Y1 - 2012/6/11
N2 - Raman spectroscopy is a non-invasive technique offering great potential in the biomedical field for label-free discrimination between normal and tumor cells based on their biochemical composition. First, this contribution describes Raman spectra of lymphocytes after drying, in laser tweezers, and trapped in a microfluidic environment. Second, spectral differences between lymphocytes and acute myeloid leukemia cells (OCI-AML3) are compared for these three experimental conditions. Significant similarities of difference spectra are consistent with the biological relevance of the spectral features. Third, modulated wavelength Raman spectroscopy has been applied to this model system to demonstrate background suppression. Here, the laser excitation wavelength of 785 nm was modulated with a frequency of 40 mHz by 0.6 nm. 40 spectra were accumulated with an exposure time of 5 seconds each. These data were subjected to principal component analysis to calculate modulated Raman signatures. The loading of the principal component shows characteristics of first derivatives with derivative like band shapes. The derivative of this loading corresponds to a pseudo-second derivative spectrum and enables to determine band positions.
AB - Raman spectroscopy is a non-invasive technique offering great potential in the biomedical field for label-free discrimination between normal and tumor cells based on their biochemical composition. First, this contribution describes Raman spectra of lymphocytes after drying, in laser tweezers, and trapped in a microfluidic environment. Second, spectral differences between lymphocytes and acute myeloid leukemia cells (OCI-AML3) are compared for these three experimental conditions. Significant similarities of difference spectra are consistent with the biological relevance of the spectral features. Third, modulated wavelength Raman spectroscopy has been applied to this model system to demonstrate background suppression. Here, the laser excitation wavelength of 785 nm was modulated with a frequency of 40 mHz by 0.6 nm. 40 spectra were accumulated with an exposure time of 5 seconds each. These data were subjected to principal component analysis to calculate modulated Raman signatures. The loading of the principal component shows characteristics of first derivatives with derivative like band shapes. The derivative of this loading corresponds to a pseudo-second derivative spectrum and enables to determine band positions.
UR - http://www.scopus.com/inward/record.url?scp=84861872158&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84861872158&partnerID=8YFLogxK
U2 - 10.1117/12.908564
DO - 10.1117/12.908564
M3 - Conference contribution
AN - SCOPUS:84861872158
SN - 9780819488626
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Biomedical Vibrational Spectroscopy V
ER -