Rapid Analysis of Bacterial Contamination in Platelets without Pre-Enrichment Using Pig Serum-Derived Antibodies

Ji Hong Bong, Jun Hee Park, Jeong Soo Sung, Chang Kyu Lee, Ga Yeon Lee, Min Jung Kang, Hyun Ok Kim, Jae Chul Pyun

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

As the shelf life of platelets collected from donated blood is very short, approximately 5 days, the determination of bacterial contamination in platelets has become necessary. In this study, rapid analysis of Gram-positive and Gram-negative bacterial contamination in platelet samples was presented without pre-enrichment using pig serum-derived antibodies against the outer membrane proteins (OMP) of Gram-negative bacteria and antibodies against lipoteichoic acid (LTA) on the surface of Gram-positive bacteria. The anti-OMP antibodies against Gram-negative bacteria were isolated using sequential incubation with (1) the modified Gram-negative bacteria ClearColi, which lacks lipopolysaccharide (LPS) on the outer membrane, and (2) the Gram-positive bacteriaBacillus subtilis to filter away nonspecifically bound proteins from ClearColi. The anti-lipoteichoic acid (LTA) antibodies against Gram-positive bacteria were isolated using sequential incubation with (1) the Gram-positive bacteriaB. subtilis and (2) the Gram-negative bacteria Escherichia coli BL21 to filter away nonspecifically bound proteins fromB. subtilis. The feasibility of using the antibodies isolated from pig serum against Gram-negative and Gram-positive bacteria was demonstrated using flow cytometry. Finally, detection of the contamination of platelets with Gram-negative and Gram-positive bacteria using the impedance immunosensor based on these isolated antibodies was successfully demonstrated.

Original languageEnglish
Pages (from-to)7779-7789
Number of pages11
JournalACS Applied Bio Materials
Volume4
Issue number11
DOIs
Publication statusPublished - 2021 Nov 15

Bibliographical note

Funding Information:
This study was supported by the National Research Foundation of Korea [grant numbers NRF-2020R1A2B5B01002187 and NRF-2020R1A5A101913111].

Publisher Copyright:
©

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Chemistry(all)
  • Biomedical Engineering
  • Biochemistry, medical

Fingerprint

Dive into the research topics of 'Rapid Analysis of Bacterial Contamination in Platelets without Pre-Enrichment Using Pig Serum-Derived Antibodies'. Together they form a unique fingerprint.

Cite this