Rapid analysis of matrix metalloproteinase-3 activity by gelatin arrays using a spectral surface plasmon resonance biosensor

Se Hui Jung, Deok Hoon Kong, Jun Hyoung Park, Seung-Taek Lee, Jinho Hyun, Young Myeong Kim, Kwon Soo Ha

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We developed a novel assay system using an array-based spectral surface plasmon resonance (SPR) biosensor for a high-throughput analysis of matrix metalloproteinase (MMP)-3 activity. Gelatin arrays were fabricated by immobilizing gelatin, a MMP-3 substrate, on amine-modified gold arrays. MMP-3 activity was determined by monitoring the shift of SPR wavelength caused by gelatin proteolysis. The gelatinolytic activity of MMP-3, which caused a decrease of the SPR wavelength, was verified by SPR spectroscopy, atomic force microscopy, and fluorescence-based protein arrays. MMP-3 activity increased by three non-ionic detergents in a dose-dependent manner, and Brij-35 was most effective. The array-based SPR biosensor was successfully applied to the rapid analysis of dose-dependent MMP-3 activity and its inhibition with tissue inhibitors of metalloproteinase 1 and GM6001, MMP inhibitors. Therefore, this new assay system using a spectral SPR biosensor is simple, label-free, and high-throughput, and is likely to have a strong potential for inhibitor screening.

Original languageEnglish
Pages (from-to)1050-1057
Number of pages8
JournalAnalyst
Volume135
Issue number5
DOIs
Publication statusPublished - 2010 May 3

Fingerprint

Matrix Metalloproteinase 3
Surface Plasmon Resonance
Surface plasmon resonance
Biosensing Techniques
Gelatin
Biosensors
matrix
inhibitor
Assays
Throughput
Proteolysis
Wavelength
Protein Array Analysis
assay
Tissue Inhibitor of Metalloproteinase-1
Matrix Metalloproteinase Inhibitors
wavelength
Atomic Force Microscopy
atomic force microscopy
Gold

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Jung, Se Hui ; Kong, Deok Hoon ; Park, Jun Hyoung ; Lee, Seung-Taek ; Hyun, Jinho ; Kim, Young Myeong ; Ha, Kwon Soo. / Rapid analysis of matrix metalloproteinase-3 activity by gelatin arrays using a spectral surface plasmon resonance biosensor. In: Analyst. 2010 ; Vol. 135, No. 5. pp. 1050-1057.
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Rapid analysis of matrix metalloproteinase-3 activity by gelatin arrays using a spectral surface plasmon resonance biosensor. / Jung, Se Hui; Kong, Deok Hoon; Park, Jun Hyoung; Lee, Seung-Taek; Hyun, Jinho; Kim, Young Myeong; Ha, Kwon Soo.

In: Analyst, Vol. 135, No. 5, 03.05.2010, p. 1050-1057.

Research output: Contribution to journalArticle

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