Rapid and sensitive detection of bacteria pathogen based on microfluidic enrichement with label-free nanobiosensor

Tae Yoon Lee, Yong Shin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A highly sensitive nanobiosensor combining a Concanavalina A (Con A) functionalized microfluidic chip is reported for rapid detection of bacteria pathogen based on isothermal amplification. The microfluidic chip including asymmetric herringbone structures was employed to enrich microorganisms of interests and extract DNAs. An optical nanobiosensor-based isothermal nucleic acid amplification technique was used to carry out the simultaneous amplification and detection of target nucleic acid in a label-free and real-time manner. Salmonella enterica serotype Typhimurium can be detected at the concentration as low as 10 colony-forming units (CFU) in 1ml PBS buffer and 50 CFU in 10 ml urine sample. The developed nanobiosensing platform has potential to provide a low-cost and sensitive tool for rapid bacterial detection in various samples.

Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages1577-1578
Number of pages2
ISBN (Electronic)9780692941836
Publication statusPublished - 2020
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: 2017 Oct 222017 Oct 26

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
CountryUnited States
CitySavannah
Period17/10/2217/10/26

Bibliographical note

Funding Information:
This work was supported by a research fund of Chungnam National University and the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the the Ministry of Education under grant number 2016R1D1A1B03934790.

Publisher Copyright:
© 17CBMS-0001.

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Fingerprint Dive into the research topics of 'Rapid and sensitive detection of bacteria pathogen based on microfluidic enrichement with label-free nanobiosensor'. Together they form a unique fingerprint.

Cite this