Rapid imaging of mammalian brain slices with a compact light sheet fluorescent microscope

Zhengyi Yang, Peter Haslehurst, Suzanne Scott, Nigel Emptage, Kishan Dholakia

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Light sheet fluorescent microscopy is able to provide high acquisition speed and high contrast images, as well as the low photo-bleaching and photo-damage brought to the sample. Here we describe a compact setup design optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We report this prototype instrument is capable of rapid imaging wide area of the dendritic or axonal arbor of a dye-filled neuron in hippocampal slice. We also show several applications of this compact light sheet fluorescent microscope, to demonstrate that our approach offers a powerful functionality to the neuroscience community that is not achievable with traditional imaging methods.

Original languageEnglish
Title of host publicationNeural Imaging and Sensing
EditorsJun Ding, Qingming Luo
ISBN (Electronic)9781510605435
Publication statusPublished - 2017
EventNeural Imaging and Sensing - San Francisco, United States
Duration: 2017 Jan 302017 Jan 31

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceNeural Imaging and Sensing
Country/TerritoryUnited States
CitySan Francisco

Bibliographical note

Publisher Copyright:
© 2017 SPIE.

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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