Rapid imaging of mammalian brain slices with a compact light sheet fluorescent microscope

Zhengyi Yang; Peter Haslehurst; Suzanne Scott; Nigel Emptage; Kishan Dholakia

doi:10.1117/12.2251227

Rapid imaging of mammalian brain slices with a compact light sheet fluorescent microscope

Zhengyi Yang, Peter Haslehurst, Suzanne Scott, Nigel Emptage, Kishan Dholakia

Department of Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Light sheet fluorescent microscopy is able to provide high acquisition speed and high contrast images, as well as the low photo-bleaching and photo-damage brought to the sample. Here we describe a compact setup design optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We report this prototype instrument is capable of rapid imaging wide area of the dendritic or axonal arbor of a dye-filled neuron in hippocampal slice. We also show several applications of this compact light sheet fluorescent microscope, to demonstrate that our approach offers a powerful functionality to the neuroscience community that is not achievable with traditional imaging methods.

Original language	English
Title of host publication	Neural Imaging and Sensing
Editors	Jun Ding, Qingming Luo
Publisher	SPIE
ISBN (Electronic)	9781510605435
DOIs	https://doi.org/10.1117/12.2251227
Publication status	Published - 2017
Event	Neural Imaging and Sensing - San Francisco, United States Duration: 2017 Jan 30 → 2017 Jan 31

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10051
ISSN (Print)	1605-7422

Conference

Conference	Neural Imaging and Sensing
Country/Territory	United States
City	San Francisco
Period	17/1/30 → 17/1/31

Bibliographical note

Publisher Copyright:
© 2017 SPIE.

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

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10.1117/12.2251227

Cite this

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title = "Rapid imaging of mammalian brain slices with a compact light sheet fluorescent microscope",

abstract = "Light sheet fluorescent microscopy is able to provide high acquisition speed and high contrast images, as well as the low photo-bleaching and photo-damage brought to the sample. Here we describe a compact setup design optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We report this prototype instrument is capable of rapid imaging wide area of the dendritic or axonal arbor of a dye-filled neuron in hippocampal slice. We also show several applications of this compact light sheet fluorescent microscope, to demonstrate that our approach offers a powerful functionality to the neuroscience community that is not achievable with traditional imaging methods.",

author = "Zhengyi Yang and Peter Haslehurst and Suzanne Scott and Nigel Emptage and Kishan Dholakia",

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year = "2017",

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language = "English",

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Yang, Z, Haslehurst, P, Scott, S, Emptage, N & Dholakia, K 2017, Rapid imaging of mammalian brain slices with a compact light sheet fluorescent microscope. in J Ding & Q Luo (eds), Neural Imaging and Sensing., 100510T, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10051, SPIE, Neural Imaging and Sensing, San Francisco, United States, 17/1/30. https://doi.org/10.1117/12.2251227

Rapid imaging of mammalian brain slices with a compact light sheet fluorescent microscope. / Yang, Zhengyi; Haslehurst, Peter; Scott, Suzanne et al.

Neural Imaging and Sensing. ed. / Jun Ding; Qingming Luo. SPIE, 2017. 100510T (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10051).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AU - Dholakia, Kishan

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AB - Light sheet fluorescent microscopy is able to provide high acquisition speed and high contrast images, as well as the low photo-bleaching and photo-damage brought to the sample. Here we describe a compact setup design optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We report this prototype instrument is capable of rapid imaging wide area of the dendritic or axonal arbor of a dye-filled neuron in hippocampal slice. We also show several applications of this compact light sheet fluorescent microscope, to demonstrate that our approach offers a powerful functionality to the neuroscience community that is not achievable with traditional imaging methods.

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PB - SPIE

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Y2 - 30 January 2017 through 31 January 2017

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Rapid imaging of mammalian brain slices with a compact light sheet fluorescent microscope

Abstract

Publication series

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Bibliographical note

All Science Journal Classification (ASJC) codes

Access to Document

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