Light sheet fluorescent microscopy is able to provide high acquisition speed and high contrast images, as well as the low photo-bleaching and photo-damage brought to the sample. Here we describe a compact setup design optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We report this prototype instrument is capable of rapid imaging wide area of the dendritic or axonal arbor of a dye-filled neuron in hippocampal slice. We also show several applications of this compact light sheet fluorescent microscope, to demonstrate that our approach offers a powerful functionality to the neuroscience community that is not achievable with traditional imaging methods.
|Title of host publication||Neural Imaging and Sensing|
|Editors||Jun Ding, Qingming Luo|
|Publication status||Published - 2017|
|Event||Neural Imaging and Sensing - San Francisco, United States|
Duration: 2017 Jan 30 → 2017 Jan 31
|Name||Progress in Biomedical Optics and Imaging - Proceedings of SPIE|
|Conference||Neural Imaging and Sensing|
|Period||17/1/30 → 17/1/31|
Bibliographical notePublisher Copyright:
© 2017 SPIE.
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging