Rapid induction of senescence in human cervical carcinoma cells

Edward C. Goodwin, Eva Yang, Chan Jae Lee, Han Woong Lee, Daniel DiMaio, Eun Seong Hwang

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144 Citations (Scopus)

Abstract

Expression of the bovine papillomavirus E2 regulatory protein in human cervical carcinoma cell lines repressed expression of the resident human papillomavirus E6 and E7 oncogenes and within a few days caused essentially all of the cells to synchronously display numerous phenotypic markers characteristic of cells undergoing replicative senescence. This process was accompanied by marked but in some cases transient alterations in the expression of cell cycle regulatory proteins and by decreased telomerase activity. We propose that the human papillomavirus E6 and E7 proteins actively prevent senescence from occurring in cervical carcinoma cells, and that once viral oncogene expression is extinguished, the senescence program is rapidly executed. Activation of endogenous senescence pathways in cancer cells may represent an alternative approach to treat human cancers.

Original languageEnglish
Pages (from-to)10978-10983
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number20
DOIs
Publication statusPublished - 2000 Sep 26

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