Abstract
Ultrahigh performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLCESI-MS/MS) provides a high-speed method to screen a large number of samples for small molecules with specific properties. In this study, UPLC-ESI-MS/MS with multiple reaction monitoring (MRM) was employed to screen urinary phospholipid (PL) content for biomarkers of prostate cancer. From lists of urinary PLs structurally identified using nanoflow LC-ESI-MS/MS, 52 PL species were selected for quantitative analysis in urine samples between 22 cancer-free urologic patients as controls and 45 prostate cancer patients. Statistical treatment of data by receiver operating characteristic (ROC) analysis yielded 14 PL species that differed significantly in relative concentrations (area under curve (AUC) > 0.8) between the two groups. Among PLs present at higher levels in prostate cancer urine, phosphatidylcholines (PCs) and phosphatidylinositols (PIs) constituted the major head group PLs (3 PCs and 7 PIs). For technical reasons, PL species of low abundance may be underrepresented in data from UPLC-ESI-MS/MS performed in MRM mode. However, the proposed method enables the rapid screening of large numbers of plasma or urine samples in the search for biomarkers of human disease.
| Original language | English |
|---|---|
| Pages (from-to) | 1133-1138 |
| Number of pages | 6 |
| Journal | Bulletin of the Korean Chemical Society |
| Volume | 35 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2014 Apr 20 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
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Rapid screening of phospholipid biomarker candidates from prostate cancer urine samples by multiple reaction monitoring of UPLC-ESI-MS/MS and statistical approaches. / Lim, Sangsoo; Bang, Dae Young; Rha, Koon Ho; Moon, Myeong Hee.
In: Bulletin of the Korean Chemical Society, Vol. 35, No. 4, 20.04.2014, p. 1133-1138.Research output: Contribution to journal › Article
TY - JOUR
T1 - Rapid screening of phospholipid biomarker candidates from prostate cancer urine samples by multiple reaction monitoring of UPLC-ESI-MS/MS and statistical approaches
AU - Lim, Sangsoo
AU - Bang, Dae Young
AU - Rha, Koon Ho
AU - Moon, Myeong Hee
PY - 2014/4/20
Y1 - 2014/4/20
N2 - Ultrahigh performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLCESI-MS/MS) provides a high-speed method to screen a large number of samples for small molecules with specific properties. In this study, UPLC-ESI-MS/MS with multiple reaction monitoring (MRM) was employed to screen urinary phospholipid (PL) content for biomarkers of prostate cancer. From lists of urinary PLs structurally identified using nanoflow LC-ESI-MS/MS, 52 PL species were selected for quantitative analysis in urine samples between 22 cancer-free urologic patients as controls and 45 prostate cancer patients. Statistical treatment of data by receiver operating characteristic (ROC) analysis yielded 14 PL species that differed significantly in relative concentrations (area under curve (AUC) > 0.8) between the two groups. Among PLs present at higher levels in prostate cancer urine, phosphatidylcholines (PCs) and phosphatidylinositols (PIs) constituted the major head group PLs (3 PCs and 7 PIs). For technical reasons, PL species of low abundance may be underrepresented in data from UPLC-ESI-MS/MS performed in MRM mode. However, the proposed method enables the rapid screening of large numbers of plasma or urine samples in the search for biomarkers of human disease.
AB - Ultrahigh performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLCESI-MS/MS) provides a high-speed method to screen a large number of samples for small molecules with specific properties. In this study, UPLC-ESI-MS/MS with multiple reaction monitoring (MRM) was employed to screen urinary phospholipid (PL) content for biomarkers of prostate cancer. From lists of urinary PLs structurally identified using nanoflow LC-ESI-MS/MS, 52 PL species were selected for quantitative analysis in urine samples between 22 cancer-free urologic patients as controls and 45 prostate cancer patients. Statistical treatment of data by receiver operating characteristic (ROC) analysis yielded 14 PL species that differed significantly in relative concentrations (area under curve (AUC) > 0.8) between the two groups. Among PLs present at higher levels in prostate cancer urine, phosphatidylcholines (PCs) and phosphatidylinositols (PIs) constituted the major head group PLs (3 PCs and 7 PIs). For technical reasons, PL species of low abundance may be underrepresented in data from UPLC-ESI-MS/MS performed in MRM mode. However, the proposed method enables the rapid screening of large numbers of plasma or urine samples in the search for biomarkers of human disease.
UR - http://www.scopus.com/inward/record.url?scp=84898648250&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84898648250&partnerID=8YFLogxK
U2 - 10.5012/bkcs.2014.35.4.1133
DO - 10.5012/bkcs.2014.35.4.1133
M3 - Article
AN - SCOPUS:84898648250
VL - 35
SP - 1133
EP - 1138
JO - Bulletin of the Korean Chemical Society
JF - Bulletin of the Korean Chemical Society
SN - 0253-2964
IS - 4
ER -