Rdp1, a novel zinc finger protein, regulates the dna damage response of rhp51+ from Schizosaccharomyces pombe

Y. S. Shim, Y. K. Jang, M. S. Lim, J. S. Lee, R. H. Seong, S. H. Hong, S. D. Park

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The Schizosaccharomyces pombe DNA repair gene rhp51+ encodes a RecA-like protein with the DNA-dependent ATPase activity required for homologous recombination. The level of the rhp51+ transcript is increased by a variety of DNA-damaging agents. Its promoter has two cis-acting DNA damage-responsive elements (DREs) responsible for DNA damage inducibility. Here we report identification of Rdp1, which regulates rhp51+ expression through the DRE of rhp51+. The protein contains a zinc finger and a polyalanine tract similar to ones previously implicated in DNA binding and transactivation or repression, respectively. In vitro footprinting and competitive binding assays indicate that the core consensus sequences (NGG/TTG/A) of DRE are crucial for the binding of Rdp1. Mutations of both DRE1 and DRE2 affected the damage-induced expression of rhp51+, indicating that both DREs are required for transcriptional activation. In addition, mutations in the DREs significantly reduced survival rates after exposure to DNA-damaging agents, demonstrating that the damage response of rhp51+ enhances the cellular repair capacity. Surprisingly, haploid cells containing a complete rdp1 deletion could not be recovered, indicating that rdp1+ is essential for cell viability and implying the existence of other target genes. Furthermore, the DNA damage-dependent expression of rhp51+ was significantly reduced in checkpoint mutants, raising the possibility that Rdp1 may mediate damage checkpoint-dependent transcription of rhp51+.

Original languageEnglish
Pages (from-to)8958-8968
Number of pages11
JournalMolecular and Cellular Biology
Volume20
Issue number23
DOIs
Publication statusPublished - 2000 Dec 1

Fingerprint

Schizosaccharomyces
Zinc Fingers
DNA Damage
Transcriptional Activation
DNA
Rec A Recombinases
Mutation
Proteins
Competitive Binding
Haploidy
Homologous Recombination
Consensus Sequence
DNA Repair
Genes
Adenosine Triphosphatases
Cell Survival

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Shim, Y. S. ; Jang, Y. K. ; Lim, M. S. ; Lee, J. S. ; Seong, R. H. ; Hong, S. H. ; Park, S. D. / Rdp1, a novel zinc finger protein, regulates the dna damage response of rhp51+ from Schizosaccharomyces pombe. In: Molecular and Cellular Biology. 2000 ; Vol. 20, No. 23. pp. 8958-8968.
@article{f95b47bd43f4424cbf169f0cf50a2265,
title = "Rdp1, a novel zinc finger protein, regulates the dna damage response of rhp51+ from Schizosaccharomyces pombe",
abstract = "The Schizosaccharomyces pombe DNA repair gene rhp51+ encodes a RecA-like protein with the DNA-dependent ATPase activity required for homologous recombination. The level of the rhp51+ transcript is increased by a variety of DNA-damaging agents. Its promoter has two cis-acting DNA damage-responsive elements (DREs) responsible for DNA damage inducibility. Here we report identification of Rdp1, which regulates rhp51+ expression through the DRE of rhp51+. The protein contains a zinc finger and a polyalanine tract similar to ones previously implicated in DNA binding and transactivation or repression, respectively. In vitro footprinting and competitive binding assays indicate that the core consensus sequences (NGG/TTG/A) of DRE are crucial for the binding of Rdp1. Mutations of both DRE1 and DRE2 affected the damage-induced expression of rhp51+, indicating that both DREs are required for transcriptional activation. In addition, mutations in the DREs significantly reduced survival rates after exposure to DNA-damaging agents, demonstrating that the damage response of rhp51+ enhances the cellular repair capacity. Surprisingly, haploid cells containing a complete rdp1 deletion could not be recovered, indicating that rdp1+ is essential for cell viability and implying the existence of other target genes. Furthermore, the DNA damage-dependent expression of rhp51+ was significantly reduced in checkpoint mutants, raising the possibility that Rdp1 may mediate damage checkpoint-dependent transcription of rhp51+.",
author = "Shim, {Y. S.} and Jang, {Y. K.} and Lim, {M. S.} and Lee, {J. S.} and Seong, {R. H.} and Hong, {S. H.} and Park, {S. D.}",
year = "2000",
month = "12",
day = "1",
doi = "10.1128/MCB.20.23.8958-8968.2000",
language = "English",
volume = "20",
pages = "8958--8968",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "23",

}

Rdp1, a novel zinc finger protein, regulates the dna damage response of rhp51+ from Schizosaccharomyces pombe. / Shim, Y. S.; Jang, Y. K.; Lim, M. S.; Lee, J. S.; Seong, R. H.; Hong, S. H.; Park, S. D.

In: Molecular and Cellular Biology, Vol. 20, No. 23, 01.12.2000, p. 8958-8968.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Rdp1, a novel zinc finger protein, regulates the dna damage response of rhp51+ from Schizosaccharomyces pombe

AU - Shim, Y. S.

AU - Jang, Y. K.

AU - Lim, M. S.

AU - Lee, J. S.

AU - Seong, R. H.

AU - Hong, S. H.

AU - Park, S. D.

PY - 2000/12/1

Y1 - 2000/12/1

N2 - The Schizosaccharomyces pombe DNA repair gene rhp51+ encodes a RecA-like protein with the DNA-dependent ATPase activity required for homologous recombination. The level of the rhp51+ transcript is increased by a variety of DNA-damaging agents. Its promoter has two cis-acting DNA damage-responsive elements (DREs) responsible for DNA damage inducibility. Here we report identification of Rdp1, which regulates rhp51+ expression through the DRE of rhp51+. The protein contains a zinc finger and a polyalanine tract similar to ones previously implicated in DNA binding and transactivation or repression, respectively. In vitro footprinting and competitive binding assays indicate that the core consensus sequences (NGG/TTG/A) of DRE are crucial for the binding of Rdp1. Mutations of both DRE1 and DRE2 affected the damage-induced expression of rhp51+, indicating that both DREs are required for transcriptional activation. In addition, mutations in the DREs significantly reduced survival rates after exposure to DNA-damaging agents, demonstrating that the damage response of rhp51+ enhances the cellular repair capacity. Surprisingly, haploid cells containing a complete rdp1 deletion could not be recovered, indicating that rdp1+ is essential for cell viability and implying the existence of other target genes. Furthermore, the DNA damage-dependent expression of rhp51+ was significantly reduced in checkpoint mutants, raising the possibility that Rdp1 may mediate damage checkpoint-dependent transcription of rhp51+.

AB - The Schizosaccharomyces pombe DNA repair gene rhp51+ encodes a RecA-like protein with the DNA-dependent ATPase activity required for homologous recombination. The level of the rhp51+ transcript is increased by a variety of DNA-damaging agents. Its promoter has two cis-acting DNA damage-responsive elements (DREs) responsible for DNA damage inducibility. Here we report identification of Rdp1, which regulates rhp51+ expression through the DRE of rhp51+. The protein contains a zinc finger and a polyalanine tract similar to ones previously implicated in DNA binding and transactivation or repression, respectively. In vitro footprinting and competitive binding assays indicate that the core consensus sequences (NGG/TTG/A) of DRE are crucial for the binding of Rdp1. Mutations of both DRE1 and DRE2 affected the damage-induced expression of rhp51+, indicating that both DREs are required for transcriptional activation. In addition, mutations in the DREs significantly reduced survival rates after exposure to DNA-damaging agents, demonstrating that the damage response of rhp51+ enhances the cellular repair capacity. Surprisingly, haploid cells containing a complete rdp1 deletion could not be recovered, indicating that rdp1+ is essential for cell viability and implying the existence of other target genes. Furthermore, the DNA damage-dependent expression of rhp51+ was significantly reduced in checkpoint mutants, raising the possibility that Rdp1 may mediate damage checkpoint-dependent transcription of rhp51+.

UR - http://www.scopus.com/inward/record.url?scp=0034462120&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034462120&partnerID=8YFLogxK

U2 - 10.1128/MCB.20.23.8958-8968.2000

DO - 10.1128/MCB.20.23.8958-8968.2000

M3 - Article

C2 - 11073995

AN - SCOPUS:0034462120

VL - 20

SP - 8958

EP - 8968

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 23

ER -