Reaction monitoring of succinylation of collagen with matrix-assisted laser desorption/ionization mass spectrometry

Sung Ho Kim, Jeoung Hwa Lee, Shin Young Yun, Jong Shin Yoo, Chul Ho Jun, Kwan Yong Chung, Hwal Suh

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15 Citations (Scopus)

Abstract

Succinylated collagen was synthesized by the reaction of collagen with succinic anhydride under basic conditions for one hour. Using the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) technique, the reaction products were directly identified without multi-step separation processes. MALDI-MS monitored the reaction more accurately than the conventional method of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). From the change in molecular mass during the reaction, it is observed that about 28 succinyl groups are attached to the collagen strand. Copyright (C) 2000 John Wiley and Sons, Ltd.

Original languageEnglish
Pages (from-to)2125-2128
Number of pages4
JournalRapid Communications in Mass Spectrometry
Volume14
Issue number22
DOIs
Publication statusPublished - 2000 Nov 30

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry

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