Reactive oxygen species induce antiviral innate immune response through IFN-λ regulation in human nasal epithelial cells

Hyun Jik Kim, Chang-Hoon Kim, Ji Hwan Ryu, Min Ji Kim, Chong Yoon Park, Jae Myun Lee, Michael J. Holtzman, Joo Heon Yoon

Research output: Contribution to journalArticle

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Abstract

This study sought to explore the role of the IFN-related innate immune responses (IFN-β and IFN-λ) and of reactive oxygen species (ROS) after influenza A virus (IAV) infection for antiviral innate immune activity in normal human nasal epithelial (NHNE) cells that are highly exposed to IAV. Passage-2 NHNE cells were inoculated with the IAV WSN/33 for 1, 2, and 3 days to assess the capacity of IFN and the relationship between ROS generation and IFN-λ secretion for controlling IAV infection. Viral titers and IAV mRNA levels increased after infection. In concert with viral titers, we found that the generation of IFNs, such as IFN-b, IFN-λ1, and IFN-λ2/3, was induced after IAV infection until 3 days after infection. The induction of IFN-λ gene expression and protein secretion may be predominant after IAV infection. Similarly, we observed that intracellular ROS generation increased60minutes afterIAV infection. Viral titersandmRNAlevels of IAV were significantly higher in cases with scavenging ROS, in cases with an induced IFN-λ mRNA level, or where the secreted protein concentration of IFN-λ was attenuated after the suppression of ROS generation. Bothmitochondrial and dual oxidase (Doux)2-generated ROSwere correlatedwith IAVmRNAandviral titers. Theinhibition of mitochondrial ROS generation and the knockdown of Duox2 gene expression highly increased IAV viral titers and decreased IFN-λ secretion. Our findings suggest that the production of ROS may be responsible for IFN-λ secretion to control IAV infection. Both mitochondria and Duox2 are possible sources of ROS generation, which is required to initiate an innate immune response in NHNE cells.

Original languageEnglish
Pages (from-to)855-865
Number of pages11
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume49
Issue number5
DOIs
Publication statusPublished - 2013 Nov 1

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Influenza A virus
Viruses
Nose
Innate Immunity
Antiviral Agents
Reactive Oxygen Species
Epithelial Cells
Virus Diseases
Gene expression
Infection
Gene Expression
Messenger RNA
Mitochondria
Scavenging
Viral Load
Oxidoreductases
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Cite this

Kim, Hyun Jik ; Kim, Chang-Hoon ; Ryu, Ji Hwan ; Kim, Min Ji ; Park, Chong Yoon ; Lee, Jae Myun ; Holtzman, Michael J. ; Yoon, Joo Heon. / Reactive oxygen species induce antiviral innate immune response through IFN-λ regulation in human nasal epithelial cells. In: American Journal of Respiratory Cell and Molecular Biology. 2013 ; Vol. 49, No. 5. pp. 855-865.
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abstract = "This study sought to explore the role of the IFN-related innate immune responses (IFN-β and IFN-λ) and of reactive oxygen species (ROS) after influenza A virus (IAV) infection for antiviral innate immune activity in normal human nasal epithelial (NHNE) cells that are highly exposed to IAV. Passage-2 NHNE cells were inoculated with the IAV WSN/33 for 1, 2, and 3 days to assess the capacity of IFN and the relationship between ROS generation and IFN-λ secretion for controlling IAV infection. Viral titers and IAV mRNA levels increased after infection. In concert with viral titers, we found that the generation of IFNs, such as IFN-b, IFN-λ1, and IFN-λ2/3, was induced after IAV infection until 3 days after infection. The induction of IFN-λ gene expression and protein secretion may be predominant after IAV infection. Similarly, we observed that intracellular ROS generation increased60minutes afterIAV infection. Viral titersandmRNAlevels of IAV were significantly higher in cases with scavenging ROS, in cases with an induced IFN-λ mRNA level, or where the secreted protein concentration of IFN-λ was attenuated after the suppression of ROS generation. Bothmitochondrial and dual oxidase (Doux)2-generated ROSwere correlatedwith IAVmRNAandviral titers. Theinhibition of mitochondrial ROS generation and the knockdown of Duox2 gene expression highly increased IAV viral titers and decreased IFN-λ secretion. Our findings suggest that the production of ROS may be responsible for IFN-λ secretion to control IAV infection. Both mitochondria and Duox2 are possible sources of ROS generation, which is required to initiate an innate immune response in NHNE cells.",
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Reactive oxygen species induce antiviral innate immune response through IFN-λ regulation in human nasal epithelial cells. / Kim, Hyun Jik; Kim, Chang-Hoon; Ryu, Ji Hwan; Kim, Min Ji; Park, Chong Yoon; Lee, Jae Myun; Holtzman, Michael J.; Yoon, Joo Heon.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 49, No. 5, 01.11.2013, p. 855-865.

Research output: Contribution to journalArticle

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