Reactive Oxygen Species-Regulating Polymersome as an Antiviral Agent against Influenza Virus

Hyun Ouk Kim, Minjoo Yeom, Jihye Kim, Aastha Kukreja, Woonsung Na, Jihye Choi, Aram Kang, Dayeon Yun, Jong Woo Lim, Daesub Song, Seungjoo Haam

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Reactive oxygen species (ROS) produced during mitochondrial oxidative phosphorylation play an important role as signal messengers in the immune system and also regulate signal transduction. ROS production, initiated as a consequence of microbial invasion, if generated at high levels, induces activation of the MEK (mitogen-activated protein kinase kinase)/ERK (extracellular signal-regulated kinase) pathway to promote cell survival and proliferation. However, viruses hijack the host cells' pathways, causing biphasic activation of the MEK/ERK cascade. Thus, regulation of ROS leads to concomitant inhibition of virus replication. In the present study, poly(aniline-co-pyrrole) polymerized nanoregulators (PASomes) to regulate intracellular ROS levels are synthesized, exploiting their oxidizing-reducing characteristics. Poly(aniline-co-pyrrole) embedded within an amphiphilic methoxy polyethylene glycol-block-polyphenylalanine copolymer (mPEG-b-pPhe) are used. It is demonstrated that the PASomes are water soluble, biocompatible, and could control ROS levels successfully in vitro, inhibiting viral replication and cell death. Furthermore, the effects of homopolymerized nanoregulators (polypyrrole assembled with mPEG-b-pPhe or polyaniline assembled with mPEG-b-pPhe) are compared with those of the PASomes. Consequently, it is confirmed that the PASomes can regulate intracellular ROS levels successfully and suppress viral infection, thereby increasing the cell survival rate.

Original languageEnglish
Article number1700818
JournalSmall
Volume13
Issue number32
DOIs
Publication statusPublished - 2017 Aug 25

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Antiviral agents
Orthomyxoviridae
Viruses
Antiviral Agents
Reactive Oxygen Species
Oxygen
Mitogen-Activated Protein Kinase Kinases
Pyrroles
Aniline
Cell Survival
Chemical activation
Cells
Signal transduction
Immune system
Oxidative Phosphorylation
Extracellular Signal-Regulated MAP Kinases
Polypyrroles
Cell death
Virus Diseases
Virus Replication

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

Cite this

Kim, Hyun Ouk ; Yeom, Minjoo ; Kim, Jihye ; Kukreja, Aastha ; Na, Woonsung ; Choi, Jihye ; Kang, Aram ; Yun, Dayeon ; Lim, Jong Woo ; Song, Daesub ; Haam, Seungjoo. / Reactive Oxygen Species-Regulating Polymersome as an Antiviral Agent against Influenza Virus. In: Small. 2017 ; Vol. 13, No. 32.
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abstract = "Reactive oxygen species (ROS) produced during mitochondrial oxidative phosphorylation play an important role as signal messengers in the immune system and also regulate signal transduction. ROS production, initiated as a consequence of microbial invasion, if generated at high levels, induces activation of the MEK (mitogen-activated protein kinase kinase)/ERK (extracellular signal-regulated kinase) pathway to promote cell survival and proliferation. However, viruses hijack the host cells' pathways, causing biphasic activation of the MEK/ERK cascade. Thus, regulation of ROS leads to concomitant inhibition of virus replication. In the present study, poly(aniline-co-pyrrole) polymerized nanoregulators (PASomes) to regulate intracellular ROS levels are synthesized, exploiting their oxidizing-reducing characteristics. Poly(aniline-co-pyrrole) embedded within an amphiphilic methoxy polyethylene glycol-block-polyphenylalanine copolymer (mPEG-b-pPhe) are used. It is demonstrated that the PASomes are water soluble, biocompatible, and could control ROS levels successfully in vitro, inhibiting viral replication and cell death. Furthermore, the effects of homopolymerized nanoregulators (polypyrrole assembled with mPEG-b-pPhe or polyaniline assembled with mPEG-b-pPhe) are compared with those of the PASomes. Consequently, it is confirmed that the PASomes can regulate intracellular ROS levels successfully and suppress viral infection, thereby increasing the cell survival rate.",
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Kim, HO, Yeom, M, Kim, J, Kukreja, A, Na, W, Choi, J, Kang, A, Yun, D, Lim, JW, Song, D & Haam, S 2017, 'Reactive Oxygen Species-Regulating Polymersome as an Antiviral Agent against Influenza Virus', Small, vol. 13, no. 32, 1700818. https://doi.org/10.1002/smll.201700818

Reactive Oxygen Species-Regulating Polymersome as an Antiviral Agent against Influenza Virus. / Kim, Hyun Ouk; Yeom, Minjoo; Kim, Jihye; Kukreja, Aastha; Na, Woonsung; Choi, Jihye; Kang, Aram; Yun, Dayeon; Lim, Jong Woo; Song, Daesub; Haam, Seungjoo.

In: Small, Vol. 13, No. 32, 1700818, 25.08.2017.

Research output: Contribution to journalArticle

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T1 - Reactive Oxygen Species-Regulating Polymersome as an Antiviral Agent against Influenza Virus

AU - Kim, Hyun Ouk

AU - Yeom, Minjoo

AU - Kim, Jihye

AU - Kukreja, Aastha

AU - Na, Woonsung

AU - Choi, Jihye

AU - Kang, Aram

AU - Yun, Dayeon

AU - Lim, Jong Woo

AU - Song, Daesub

AU - Haam, Seungjoo

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