Reactivity of German cockroach allergen, bla g 2, peptide fragments to IgE antibodies in patients' sera

Haeseok Lee, Kyoung Yong Jeong, Kwang Hyun Shin, Myung Hee Yi, Darambazar Gantulaga, Chein Soo Hong, Tai Soon Yong

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Abstract

Bla g 2 is a cockroach allergen of great importance. This study was conducted to identify IgE-binding epitope(s) of Bla g 2 using the recombinant protein technique. Approximately 50% of tested sera showed IgE reactivity to Pichia-expressed Bla g 2 (PrBla g 2) and E. coli-expressed Bla g 2 (ErBla g 2). Only 5.3% of serum samples showed stronger reactivity to PrBla g 2 than ErBla g 2, indicating that serum was reactive to conformational or carbohydrate epitopes. The full-length and 5 peptide fragments of Bla g 2 were produced in E. coli. All fragments showed IgE-binding activity to the cockroach-allergy patients' sera. Specifically, peptide fragments of amino acid residue 1-75 and 146-225 appeared to be important for IgE-binding. The information about the IgE-binding epitope of Bla g 2 can aid in the diagnosis and treatment for cockroach allergies.

Original languageEnglish
Pages (from-to)243-246
Number of pages4
JournalKorean Journal of Parasitology
Volume46
Issue number4
DOIs
Publication statusPublished - 2008 Dec 1

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All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

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