Real-time detection of food-borne bacterial adenosine triphosphate (ATP) using dielectrophoretic force and a bioluminescence sensor

Hui Sung Moon, Hee Taek Im, Ahmi Choi, Hyo Il Jung

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Real-time detection and quantification of food-borne bacteria draws increasing interest for evaluation of food quality and safety. Since living cells invariably contain adenosine triphosphate (ATP), the detection of bacterial ATP presents a fascinating method to determine its presence in food. Care must be taken however, to remove food-derived extracellular ATP, which will interfere with detection by ATP-luminescence. We developed a microfluidic and dielectrophoretic (DEP) device for intracellular ATP detection, which captures microorganisms by DEP force and washes extracellular ATP away. The yield of capture by DEP force at a 10 μL{bullet operator}min-1 flow rate used in experiments was 87.7%. At constant ATP level the electrical sensor responded in proportion to the bacterial concentration. With a constant bacterial concentration and varying ATP, the signal did not change. These results show that the device can remove the extracellular ATP contribution from food to be sampled.

Original languageEnglish
Pages (from-to)283-288
Number of pages6
JournalMicrochimica Acta
Volume170
Issue number3
DOIs
Publication statusPublished - 2010 May 21

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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