Real-time label-free quantitative monitoring of biomolecules without surface binding by floating-gate complementary metal-oxide semiconductor sensor array integrated with readout circuitry

Seong Jin Kim, Kyutae Yoo, Jeoyoung Shim, Wonseok Chung, Christopher Ko, Maesoon Im, Lee Sup Kim, Euisik Yoon

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

We report a label-free field-effect sensing array integrated with complementary metal-oxide semiconductor (CMOS) readout circuitry to detect the surface potential determined by the negative charge in DNA molecules. For real-time DNA quantification, we have demonstrated the measurements of DNA molecules without immobilizing them on the sensing surface which is composed of an array of floating-gate CMOS transistors. This nonimmobilizing technique allows the continuous monitoring of the amount of charged molecules by injecting DNA solutions sequentially. We have carried out the real-time quantitative measurement of 19 bp oligonucleotides and analyzed its sensitivity as a function of pH in buffer solutions.

Original languageEnglish
Article number203903
JournalApplied Physics Letters
Volume91
Issue number20
DOIs
Publication statusPublished - 2007

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)

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