Abstract
Three-dimensional (3D) cell cultures have recently received attention because they represent a more physiologically relevant environment compared to conventional two-dimensional (2D) cell cultures. However, 2D-based imaging techniques or cell sensors are insufficient for real-time monitoring of cellular behavior in 3D cell culture. Here, we report investigations conducted with a 3D capacitance cell sensor consisting of vertically aligned pairs of electrodes. When GFP-expressing human breast cancer cells (GFP-MCF-7) encapsulated in alginate hydrogel were cultured in a 3D cell culture system, cellular activities, such as cell proliferation and apoptosis at different heights, could be monitored non-invasively and in real-time by measuring the change in capacitance with the 3D capacitance sensor. Moreover, we were able to monitor cell migration of human mesenchymal stem cells (hMSCs) with our 3D capacitance sensor.
| Original language | English |
|---|---|
| Pages (from-to) | 56-61 |
| Number of pages | 6 |
| Journal | Biosensors and Bioelectronics |
| Volume | 77 |
| DOIs | |
| Publication status | Published - 2016 Mar 15 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Biophysics
- Biomedical Engineering
- Electrochemistry
Cite this
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Real-time monitoring of 3D cell culture using a 3D capacitance biosensor. / Lee, Sun Mi; Han, Nalae; Lee, Rimi; Choi, In Hong; Park, Yong Beom; Shin, Jeon Soo; Yoo, Kyung Hwa.
In: Biosensors and Bioelectronics, Vol. 77, 15.03.2016, p. 56-61.Research output: Contribution to journal › Article
TY - JOUR
T1 - Real-time monitoring of 3D cell culture using a 3D capacitance biosensor
AU - Lee, Sun Mi
AU - Han, Nalae
AU - Lee, Rimi
AU - Choi, In Hong
AU - Park, Yong Beom
AU - Shin, Jeon Soo
AU - Yoo, Kyung Hwa
PY - 2016/3/15
Y1 - 2016/3/15
N2 - Three-dimensional (3D) cell cultures have recently received attention because they represent a more physiologically relevant environment compared to conventional two-dimensional (2D) cell cultures. However, 2D-based imaging techniques or cell sensors are insufficient for real-time monitoring of cellular behavior in 3D cell culture. Here, we report investigations conducted with a 3D capacitance cell sensor consisting of vertically aligned pairs of electrodes. When GFP-expressing human breast cancer cells (GFP-MCF-7) encapsulated in alginate hydrogel were cultured in a 3D cell culture system, cellular activities, such as cell proliferation and apoptosis at different heights, could be monitored non-invasively and in real-time by measuring the change in capacitance with the 3D capacitance sensor. Moreover, we were able to monitor cell migration of human mesenchymal stem cells (hMSCs) with our 3D capacitance sensor.
AB - Three-dimensional (3D) cell cultures have recently received attention because they represent a more physiologically relevant environment compared to conventional two-dimensional (2D) cell cultures. However, 2D-based imaging techniques or cell sensors are insufficient for real-time monitoring of cellular behavior in 3D cell culture. Here, we report investigations conducted with a 3D capacitance cell sensor consisting of vertically aligned pairs of electrodes. When GFP-expressing human breast cancer cells (GFP-MCF-7) encapsulated in alginate hydrogel were cultured in a 3D cell culture system, cellular activities, such as cell proliferation and apoptosis at different heights, could be monitored non-invasively and in real-time by measuring the change in capacitance with the 3D capacitance sensor. Moreover, we were able to monitor cell migration of human mesenchymal stem cells (hMSCs) with our 3D capacitance sensor.
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UR - http://www.scopus.com/inward/citedby.url?scp=84941985409&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2015.09.005
DO - 10.1016/j.bios.2015.09.005
M3 - Article
C2 - 26386332
AN - SCOPUS:84941985409
VL - 77
SP - 56
EP - 61
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
ER -