Real-time monitoring of 3D cell culture using a 3D capacitance biosensor

Sun Mi Lee, Nalae Han, Rimi Lee, In Hong Choi, Yong Beom Park, Jeon Soo Shin, Kyung Hwa Yoo

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Three-dimensional (3D) cell cultures have recently received attention because they represent a more physiologically relevant environment compared to conventional two-dimensional (2D) cell cultures. However, 2D-based imaging techniques or cell sensors are insufficient for real-time monitoring of cellular behavior in 3D cell culture. Here, we report investigations conducted with a 3D capacitance cell sensor consisting of vertically aligned pairs of electrodes. When GFP-expressing human breast cancer cells (GFP-MCF-7) encapsulated in alginate hydrogel were cultured in a 3D cell culture system, cellular activities, such as cell proliferation and apoptosis at different heights, could be monitored non-invasively and in real-time by measuring the change in capacitance with the 3D capacitance sensor. Moreover, we were able to monitor cell migration of human mesenchymal stem cells (hMSCs) with our 3D capacitance sensor.

Original languageEnglish
Pages (from-to)56-61
Number of pages6
JournalBiosensors and Bioelectronics
Volume77
DOIs
Publication statusPublished - 2016 Mar 15

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Biosensing Techniques
Cell culture
Biosensors
Capacitance
Cell Culture Techniques
Monitoring
Sensors
Hydrogel
Alginate
MCF-7 Cells
Cell proliferation
Cell death
Stem cells
Mesenchymal Stromal Cells
Hydrogels
Cell Movement
Electrodes
Cells
Cell Proliferation
Apoptosis

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Lee, Sun Mi ; Han, Nalae ; Lee, Rimi ; Choi, In Hong ; Park, Yong Beom ; Shin, Jeon Soo ; Yoo, Kyung Hwa. / Real-time monitoring of 3D cell culture using a 3D capacitance biosensor. In: Biosensors and Bioelectronics. 2016 ; Vol. 77. pp. 56-61.
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Real-time monitoring of 3D cell culture using a 3D capacitance biosensor. / Lee, Sun Mi; Han, Nalae; Lee, Rimi; Choi, In Hong; Park, Yong Beom; Shin, Jeon Soo; Yoo, Kyung Hwa.

In: Biosensors and Bioelectronics, Vol. 77, 15.03.2016, p. 56-61.

Research output: Contribution to journalArticle

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