Real-time monitoring of adipocyte differentiation using a capacitance sensor array

Rimi Lee, Inji Jung, Miyoung Park, Hunjoo Ha, Kyung Hwa Yoo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

As obesity and its associated metabolic diseases become a worldwide epidemic, the demand for novel anti-obesity agents is increasing. We report a label-free and real-time monitoring method that uses a capacitance sensor array to screen anti-obesity agents. The results for the real-time capacitance of 3T3-L1 cells treated with 12 different chemicals extracted from natural products were consistent with the biochemical indicators of adipogenesis such as the expression of perilipin, the major protein coating the surface of lipid droplets in adipocytes. The data demonstrate that a capacitance change during adipocyte differentiation is closely associated with lipid accumulation in the cells, suggesting that adipocyte differentiation can be monitored in real time. This capacitance sensor might be used for label-free and real-time monitoring of adipocyte differentiation, and may facilitate the development of high throughput screening methods for anti-obesity drugs.

Original languageEnglish
Pages (from-to)3410-3416
Number of pages7
JournalLab on a Chip
Volume13
Issue number17
DOIs
Publication statusPublished - 2013 Sep 7

Fingerprint

Sensor arrays
Anti-Obesity Agents
Adipocytes
Capacitance
Monitoring
Lipids
Labels
High-Throughput Screening Assays
3T3-L1 Cells
Adipogenesis
Metabolic Diseases
Biological Products
Screening
Obesity
Throughput
Proteins
Coatings
Sensors

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Lee, Rimi ; Jung, Inji ; Park, Miyoung ; Ha, Hunjoo ; Yoo, Kyung Hwa. / Real-time monitoring of adipocyte differentiation using a capacitance sensor array. In: Lab on a Chip. 2013 ; Vol. 13, No. 17. pp. 3410-3416.
@article{03d50071eff947ba8b21b7ff6ed18bb9,
title = "Real-time monitoring of adipocyte differentiation using a capacitance sensor array",
abstract = "As obesity and its associated metabolic diseases become a worldwide epidemic, the demand for novel anti-obesity agents is increasing. We report a label-free and real-time monitoring method that uses a capacitance sensor array to screen anti-obesity agents. The results for the real-time capacitance of 3T3-L1 cells treated with 12 different chemicals extracted from natural products were consistent with the biochemical indicators of adipogenesis such as the expression of perilipin, the major protein coating the surface of lipid droplets in adipocytes. The data demonstrate that a capacitance change during adipocyte differentiation is closely associated with lipid accumulation in the cells, suggesting that adipocyte differentiation can be monitored in real time. This capacitance sensor might be used for label-free and real-time monitoring of adipocyte differentiation, and may facilitate the development of high throughput screening methods for anti-obesity drugs.",
author = "Rimi Lee and Inji Jung and Miyoung Park and Hunjoo Ha and Yoo, {Kyung Hwa}",
year = "2013",
month = "9",
day = "7",
doi = "10.1039/c3lc50453k",
language = "English",
volume = "13",
pages = "3410--3416",
journal = "Lab on a Chip - Miniaturisation for Chemistry and Biology",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
number = "17",

}

Real-time monitoring of adipocyte differentiation using a capacitance sensor array. / Lee, Rimi; Jung, Inji; Park, Miyoung; Ha, Hunjoo; Yoo, Kyung Hwa.

In: Lab on a Chip, Vol. 13, No. 17, 07.09.2013, p. 3410-3416.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Real-time monitoring of adipocyte differentiation using a capacitance sensor array

AU - Lee, Rimi

AU - Jung, Inji

AU - Park, Miyoung

AU - Ha, Hunjoo

AU - Yoo, Kyung Hwa

PY - 2013/9/7

Y1 - 2013/9/7

N2 - As obesity and its associated metabolic diseases become a worldwide epidemic, the demand for novel anti-obesity agents is increasing. We report a label-free and real-time monitoring method that uses a capacitance sensor array to screen anti-obesity agents. The results for the real-time capacitance of 3T3-L1 cells treated with 12 different chemicals extracted from natural products were consistent with the biochemical indicators of adipogenesis such as the expression of perilipin, the major protein coating the surface of lipid droplets in adipocytes. The data demonstrate that a capacitance change during adipocyte differentiation is closely associated with lipid accumulation in the cells, suggesting that adipocyte differentiation can be monitored in real time. This capacitance sensor might be used for label-free and real-time monitoring of adipocyte differentiation, and may facilitate the development of high throughput screening methods for anti-obesity drugs.

AB - As obesity and its associated metabolic diseases become a worldwide epidemic, the demand for novel anti-obesity agents is increasing. We report a label-free and real-time monitoring method that uses a capacitance sensor array to screen anti-obesity agents. The results for the real-time capacitance of 3T3-L1 cells treated with 12 different chemicals extracted from natural products were consistent with the biochemical indicators of adipogenesis such as the expression of perilipin, the major protein coating the surface of lipid droplets in adipocytes. The data demonstrate that a capacitance change during adipocyte differentiation is closely associated with lipid accumulation in the cells, suggesting that adipocyte differentiation can be monitored in real time. This capacitance sensor might be used for label-free and real-time monitoring of adipocyte differentiation, and may facilitate the development of high throughput screening methods for anti-obesity drugs.

UR - http://www.scopus.com/inward/record.url?scp=84881069922&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881069922&partnerID=8YFLogxK

U2 - 10.1039/c3lc50453k

DO - 10.1039/c3lc50453k

M3 - Article

VL - 13

SP - 3410

EP - 3416

JO - Lab on a Chip - Miniaturisation for Chemistry and Biology

JF - Lab on a Chip - Miniaturisation for Chemistry and Biology

SN - 1473-0197

IS - 17

ER -