Disseminated tumor cells in the bone marrow environment are the main cause of systemic metastasis after curative treatment for major solid tumors. However, the detailed biological processes of tumor biology in bone marrow have not been well defined in a real-time manner, because of a lack of a proper in vivo experimental model thereof. In this study, we established intravital imaging models of the bone marrow environment to enable real-time observation of cancer cells in the bone marrow. Using these novel imaging models of intact bone marrow and transplanted bone marrow of mice, respectively, via two-photon microscopy, we could first successfully track and analyze both the distribution and the phenotype of cancer cells in bone marrow of live mouse. Therefore, these novel in vivo imaging models for the bone marrow would provide a valuable tool to identify the biologic processes of cancer cells in a real-time manner in a live animal model.
Bibliographical noteFunding Information:
We thank Jae Gwan Kim of the biophotonics lab at Gwangju Institute of Science and Technology who provided technical assistance and advice on the dorsal chamber technique. We also appreciate technical support from Yonsei Advanced Imaging Center in Avison Biomedical Research Center. This study was supported by a faculty research grant from Yonsei University College of Medicine (6-2016-0132), a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (2016R1A2B4008199; Y-MH) and the Mid-Career Researcher Program through a National Research Foundation of Korea grant (No. 2016R1A2B4011115; NC).
© 2018 Lee, Park, Zou, Seo, Jun, Lee, Hyun and Cho.
All Science Journal Classification (ASJC) codes
- Immunology and Allergy