Abstract
Background: Sepsis is one of the main causes of mortality and morbidity. The rapid detection of pathogens in blood of septic patients is essential for adequate antimicrobial therapy and better prognosis. This study aimed to accelerate the detection and discrimination of Gram-positive (GP) and Gram-negative (GN) bacteria and Candida species in blood culture samples by molecular methods.Methods: The Real-GP®, -GN®, and -CAN® real-time PCR kit (M&D, Wonju, Republic of Korea) assays use the TaqMan probes for detecting pan-GP, pan-GN, and pan-Candida species, respectively. The diagnostic performances of the real-time PCR kits were evaluated with 115 clinical isolates, 256 positive and 200 negative blood culture bottle samples, and the data were compared to results obtained from conventional blood culture.Results: Eighty-seven reference strains and 115 clinical isolates were correctly identified with specific probes corresponding to GP-bacteria, GN-bacteria and Candida, respectively. The overall sensitivity and specificity of the real-time PCR kit with blood culture samples were 99.6% and 89.5%, respectively.Conclusions: The Real-GP®, -GN®, and -CAN® real-time PCR kits could be useful tools for the rapid and accurate screening of bloodstream infections (BSIs).
| Original language | English |
|---|---|
| Article number | 3 |
| Journal | Annals of Clinical Microbiology and Antimicrobials |
| Volume | 13 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2014 Jan 7 |
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All Science Journal Classification (ASJC) codes
- Microbiology (medical)
- Infectious Diseases
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Real-time PCR taqman assay for rapid screening of bloodstream infection. / Wang, Hye young; Kim, Sunghyun; Kim, Hyunjung; Kim, Jungho; Kim, Yeun; Park, Soon Deok; Jin, Hyunwoo; Choi, Yeonim; Uh, Young; Lee, Hyeyoung.
In: Annals of Clinical Microbiology and Antimicrobials, Vol. 13, No. 1, 3, 07.01.2014.Research output: Contribution to journal › Article
TY - JOUR
T1 - Real-time PCR taqman assay for rapid screening of bloodstream infection
AU - Wang, Hye young
AU - Kim, Sunghyun
AU - Kim, Hyunjung
AU - Kim, Jungho
AU - Kim, Yeun
AU - Park, Soon Deok
AU - Jin, Hyunwoo
AU - Choi, Yeonim
AU - Uh, Young
AU - Lee, Hyeyoung
PY - 2014/1/7
Y1 - 2014/1/7
N2 - Background: Sepsis is one of the main causes of mortality and morbidity. The rapid detection of pathogens in blood of septic patients is essential for adequate antimicrobial therapy and better prognosis. This study aimed to accelerate the detection and discrimination of Gram-positive (GP) and Gram-negative (GN) bacteria and Candida species in blood culture samples by molecular methods.Methods: The Real-GP®, -GN®, and -CAN® real-time PCR kit (M&D, Wonju, Republic of Korea) assays use the TaqMan probes for detecting pan-GP, pan-GN, and pan-Candida species, respectively. The diagnostic performances of the real-time PCR kits were evaluated with 115 clinical isolates, 256 positive and 200 negative blood culture bottle samples, and the data were compared to results obtained from conventional blood culture.Results: Eighty-seven reference strains and 115 clinical isolates were correctly identified with specific probes corresponding to GP-bacteria, GN-bacteria and Candida, respectively. The overall sensitivity and specificity of the real-time PCR kit with blood culture samples were 99.6% and 89.5%, respectively.Conclusions: The Real-GP®, -GN®, and -CAN® real-time PCR kits could be useful tools for the rapid and accurate screening of bloodstream infections (BSIs).
AB - Background: Sepsis is one of the main causes of mortality and morbidity. The rapid detection of pathogens in blood of septic patients is essential for adequate antimicrobial therapy and better prognosis. This study aimed to accelerate the detection and discrimination of Gram-positive (GP) and Gram-negative (GN) bacteria and Candida species in blood culture samples by molecular methods.Methods: The Real-GP®, -GN®, and -CAN® real-time PCR kit (M&D, Wonju, Republic of Korea) assays use the TaqMan probes for detecting pan-GP, pan-GN, and pan-Candida species, respectively. The diagnostic performances of the real-time PCR kits were evaluated with 115 clinical isolates, 256 positive and 200 negative blood culture bottle samples, and the data were compared to results obtained from conventional blood culture.Results: Eighty-seven reference strains and 115 clinical isolates were correctly identified with specific probes corresponding to GP-bacteria, GN-bacteria and Candida, respectively. The overall sensitivity and specificity of the real-time PCR kit with blood culture samples were 99.6% and 89.5%, respectively.Conclusions: The Real-GP®, -GN®, and -CAN® real-time PCR kits could be useful tools for the rapid and accurate screening of bloodstream infections (BSIs).
UR - http://www.scopus.com/inward/record.url?scp=84892142728&partnerID=8YFLogxK
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U2 - 10.1186/1476-0711-13-3
DO - 10.1186/1476-0711-13-3
M3 - Article
C2 - 24393579
AN - SCOPUS:84892142728
VL - 13
JO - Annals of Clinical Microbiology and Antimicrobials
JF - Annals of Clinical Microbiology and Antimicrobials
SN - 1476-0711
IS - 1
M1 - 3
ER -