Real-time PCR taqman assay for rapid screening of bloodstream infection

Hye young Wang, Sunghyun Kim, Hyunjung Kim, Jungho Kim, Yeun Kim, Soon Deok Park, Hyunwoo Jin, Yeonim Choi, Young Uh, Hyeyoung Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Sepsis is one of the main causes of mortality and morbidity. The rapid detection of pathogens in blood of septic patients is essential for adequate antimicrobial therapy and better prognosis. This study aimed to accelerate the detection and discrimination of Gram-positive (GP) and Gram-negative (GN) bacteria and Candida species in blood culture samples by molecular methods.Methods: The Real-GP®, -GN®, and -CAN® real-time PCR kit (M&D, Wonju, Republic of Korea) assays use the TaqMan probes for detecting pan-GP, pan-GN, and pan-Candida species, respectively. The diagnostic performances of the real-time PCR kits were evaluated with 115 clinical isolates, 256 positive and 200 negative blood culture bottle samples, and the data were compared to results obtained from conventional blood culture.Results: Eighty-seven reference strains and 115 clinical isolates were correctly identified with specific probes corresponding to GP-bacteria, GN-bacteria and Candida, respectively. The overall sensitivity and specificity of the real-time PCR kit with blood culture samples were 99.6% and 89.5%, respectively.Conclusions: The Real-GP®, -GN®, and -CAN® real-time PCR kits could be useful tools for the rapid and accurate screening of bloodstream infections (BSIs).

Original languageEnglish
Article number3
JournalAnnals of Clinical Microbiology and Antimicrobials
Volume13
Issue number1
DOIs
Publication statusPublished - 2014 Jan 7

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Real-Time Polymerase Chain Reaction
Candida
Infection
Gram-Negative Bacteria
Republic of Korea
Gram-Positive Bacteria
Sepsis
Morbidity
Sensitivity and Specificity
Mortality
Blood Culture
Therapeutics

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Wang, Hye young ; Kim, Sunghyun ; Kim, Hyunjung ; Kim, Jungho ; Kim, Yeun ; Park, Soon Deok ; Jin, Hyunwoo ; Choi, Yeonim ; Uh, Young ; Lee, Hyeyoung. / Real-time PCR taqman assay for rapid screening of bloodstream infection. In: Annals of Clinical Microbiology and Antimicrobials. 2014 ; Vol. 13, No. 1.
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Real-time PCR taqman assay for rapid screening of bloodstream infection. / Wang, Hye young; Kim, Sunghyun; Kim, Hyunjung; Kim, Jungho; Kim, Yeun; Park, Soon Deok; Jin, Hyunwoo; Choi, Yeonim; Uh, Young; Lee, Hyeyoung.

In: Annals of Clinical Microbiology and Antimicrobials, Vol. 13, No. 1, 3, 07.01.2014.

Research output: Contribution to journalArticle

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AU - Wang, Hye young

AU - Kim, Sunghyun

AU - Kim, Hyunjung

AU - Kim, Jungho

AU - Kim, Yeun

AU - Park, Soon Deok

AU - Jin, Hyunwoo

AU - Choi, Yeonim

AU - Uh, Young

AU - Lee, Hyeyoung

PY - 2014/1/7

Y1 - 2014/1/7

N2 - Background: Sepsis is one of the main causes of mortality and morbidity. The rapid detection of pathogens in blood of septic patients is essential for adequate antimicrobial therapy and better prognosis. This study aimed to accelerate the detection and discrimination of Gram-positive (GP) and Gram-negative (GN) bacteria and Candida species in blood culture samples by molecular methods.Methods: The Real-GP®, -GN®, and -CAN® real-time PCR kit (M&D, Wonju, Republic of Korea) assays use the TaqMan probes for detecting pan-GP, pan-GN, and pan-Candida species, respectively. The diagnostic performances of the real-time PCR kits were evaluated with 115 clinical isolates, 256 positive and 200 negative blood culture bottle samples, and the data were compared to results obtained from conventional blood culture.Results: Eighty-seven reference strains and 115 clinical isolates were correctly identified with specific probes corresponding to GP-bacteria, GN-bacteria and Candida, respectively. The overall sensitivity and specificity of the real-time PCR kit with blood culture samples were 99.6% and 89.5%, respectively.Conclusions: The Real-GP®, -GN®, and -CAN® real-time PCR kits could be useful tools for the rapid and accurate screening of bloodstream infections (BSIs).

AB - Background: Sepsis is one of the main causes of mortality and morbidity. The rapid detection of pathogens in blood of septic patients is essential for adequate antimicrobial therapy and better prognosis. This study aimed to accelerate the detection and discrimination of Gram-positive (GP) and Gram-negative (GN) bacteria and Candida species in blood culture samples by molecular methods.Methods: The Real-GP®, -GN®, and -CAN® real-time PCR kit (M&D, Wonju, Republic of Korea) assays use the TaqMan probes for detecting pan-GP, pan-GN, and pan-Candida species, respectively. The diagnostic performances of the real-time PCR kits were evaluated with 115 clinical isolates, 256 positive and 200 negative blood culture bottle samples, and the data were compared to results obtained from conventional blood culture.Results: Eighty-seven reference strains and 115 clinical isolates were correctly identified with specific probes corresponding to GP-bacteria, GN-bacteria and Candida, respectively. The overall sensitivity and specificity of the real-time PCR kit with blood culture samples were 99.6% and 89.5%, respectively.Conclusions: The Real-GP®, -GN®, and -CAN® real-time PCR kits could be useful tools for the rapid and accurate screening of bloodstream infections (BSIs).

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