Real-time quantitative monitoring of specific peptide cleavage by a proteinase for cancer diagnosis

Gyudo Lee, Kilho Eom, Joseph Park, Jaemoon Yang, Seungjoo Haam, Yong Min Huh, Joo Kyung Ryu, Nam Hee Kim, Jong In Yook, Sang Woo Lee, Dae Sung Yoon, Taeyun Kwon

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Good vibrations: A bioassay using a resonant peptide-functionalized microcantilever enables the quantitative characterization of the proteolytic activity of membrane type-1 matrix metalloproteinase (MT1-MMP). In this assay, shifts in the frequency of the cantilever after specific proteolytic cleavage of the target peptides by MT1-MMP are measured (see picture).

Original languageEnglish
Pages (from-to)5837-5841
Number of pages5
JournalAngewandte Chemie - International Edition
Volume51
Issue number24
DOIs
Publication statusPublished - 2012 Jun 11

Fingerprint

Matrix Metalloproteinase 14
Peptides
Peptide Hydrolases
Monitoring
Bioassay
Assays
Membranes
Metalloproteases

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)

Cite this

Lee, Gyudo ; Eom, Kilho ; Park, Joseph ; Yang, Jaemoon ; Haam, Seungjoo ; Huh, Yong Min ; Ryu, Joo Kyung ; Kim, Nam Hee ; Yook, Jong In ; Lee, Sang Woo ; Yoon, Dae Sung ; Kwon, Taeyun. / Real-time quantitative monitoring of specific peptide cleavage by a proteinase for cancer diagnosis. In: Angewandte Chemie - International Edition. 2012 ; Vol. 51, No. 24. pp. 5837-5841.
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author = "Gyudo Lee and Kilho Eom and Joseph Park and Jaemoon Yang and Seungjoo Haam and Huh, {Yong Min} and Ryu, {Joo Kyung} and Kim, {Nam Hee} and Yook, {Jong In} and Lee, {Sang Woo} and Yoon, {Dae Sung} and Taeyun Kwon",
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Lee, G, Eom, K, Park, J, Yang, J, Haam, S, Huh, YM, Ryu, JK, Kim, NH, Yook, JI, Lee, SW, Yoon, DS & Kwon, T 2012, 'Real-time quantitative monitoring of specific peptide cleavage by a proteinase for cancer diagnosis', Angewandte Chemie - International Edition, vol. 51, no. 24, pp. 5837-5841. https://doi.org/10.1002/anie.201108830

Real-time quantitative monitoring of specific peptide cleavage by a proteinase for cancer diagnosis. / Lee, Gyudo; Eom, Kilho; Park, Joseph; Yang, Jaemoon; Haam, Seungjoo; Huh, Yong Min; Ryu, Joo Kyung; Kim, Nam Hee; Yook, Jong In; Lee, Sang Woo; Yoon, Dae Sung; Kwon, Taeyun.

In: Angewandte Chemie - International Edition, Vol. 51, No. 24, 11.06.2012, p. 5837-5841.

Research output: Contribution to journalArticle

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AU - Ryu, Joo Kyung

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AU - Kwon, Taeyun

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