Reappraisal of HBV genotypes and clinical significance in Koreans using MALDI-TOF mass spectrometry

Jung Min Lee, SangHoon Ahn, Hye Young Chang, Ji Eun Shin, doyoung kim, Myoung Ki Sim, Sun Pyo Hong, Hyun Jae Chung, Soo Ok Kim, KwangHyub Han, Chae Yoon Chon, Young Myoung Moon

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Abstract

BACKGROUND/AIMS: Recent studies have shown that the genotype of hepatitis B virus (HBV) may correlate with the disease natural history and treatment outcome. However, several of these studies used low sensitivity assays in a small number of patients, and this has precluded an accurate evaluation of Korean HBV genotypes. We analyzed Korean HBV genotypes in a large population by employing a new technology, restriction fragment mass polymorphism (RFMP) using MALDI-TOF mass spectrometry, in a sensitive and specific manner. METHODS: Between February 1995 and December 2003, a total of 475 patients with chronic HBV infection were enrolled. The assay is based on the mass measurement of oligonucleotides having genotypic variations of the S gene. Clinical features including the virologic status and disease progression were also evaluated. RESULTS: The median age of the total patients was 35.5 years. Out of 475 patients, there were 162 (34.1%) inactive carriers, 172 (36.2%) had chronic hepatitis, 77 (16.2%) had liver cirrhosis and 64 (13.5%) had hepatocellular carcinoma (HCC). There were 454 patients (95.6%) with genotype C, 4 patients (0.8%) with genotype A, 16 patients (3.4%) with the mixed A and C genotype [7 patients (1.4%) with A<C versus 9 patients (2.0%) with C>A], and 1 patient (0.2%) with B genotype. Comparing genotype A and C, genotype A patients were all inactive carriers without HCC, whereas genotype C patients included those with chronic hepatitis, liver cirrhosis and HCC. CONCLUSIONS: HBV genotype C is highly prevalent in Korea. Although it is a small percentage, genotype A also exists and it seems to take a more benign clinical course than genotype C. Further studies are necessitated to assess the relationship between HBV genotypes and the various aspects of the diseases' clinical course.

Original languageEnglish
Pages (from-to)260-270
Number of pages11
JournalThe Korean journal of hepatology
Volume10
Issue number4
Publication statusPublished - 2004 Jan 1

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Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Hepatitis B virus
Mass Spectrometry
Genotype
Hepatocellular Carcinoma
Chronic Hepatitis
Liver Cirrhosis
Chronic Hepatitis B
Virus Diseases
Korea
Natural History
Oligonucleotides
Disease Progression

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Lee, Jung Min ; Ahn, SangHoon ; Chang, Hye Young ; Shin, Ji Eun ; kim, doyoung ; Sim, Myoung Ki ; Hong, Sun Pyo ; Chung, Hyun Jae ; Kim, Soo Ok ; Han, KwangHyub ; Chon, Chae Yoon ; Moon, Young Myoung. / Reappraisal of HBV genotypes and clinical significance in Koreans using MALDI-TOF mass spectrometry. In: The Korean journal of hepatology. 2004 ; Vol. 10, No. 4. pp. 260-270.
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title = "Reappraisal of HBV genotypes and clinical significance in Koreans using MALDI-TOF mass spectrometry",
abstract = "BACKGROUND/AIMS: Recent studies have shown that the genotype of hepatitis B virus (HBV) may correlate with the disease natural history and treatment outcome. However, several of these studies used low sensitivity assays in a small number of patients, and this has precluded an accurate evaluation of Korean HBV genotypes. We analyzed Korean HBV genotypes in a large population by employing a new technology, restriction fragment mass polymorphism (RFMP) using MALDI-TOF mass spectrometry, in a sensitive and specific manner. METHODS: Between February 1995 and December 2003, a total of 475 patients with chronic HBV infection were enrolled. The assay is based on the mass measurement of oligonucleotides having genotypic variations of the S gene. Clinical features including the virologic status and disease progression were also evaluated. RESULTS: The median age of the total patients was 35.5 years. Out of 475 patients, there were 162 (34.1{\%}) inactive carriers, 172 (36.2{\%}) had chronic hepatitis, 77 (16.2{\%}) had liver cirrhosis and 64 (13.5{\%}) had hepatocellular carcinoma (HCC). There were 454 patients (95.6{\%}) with genotype C, 4 patients (0.8{\%}) with genotype A, 16 patients (3.4{\%}) with the mixed A and C genotype [7 patients (1.4{\%}) with A<C versus 9 patients (2.0{\%}) with C>A], and 1 patient (0.2{\%}) with B genotype. Comparing genotype A and C, genotype A patients were all inactive carriers without HCC, whereas genotype C patients included those with chronic hepatitis, liver cirrhosis and HCC. CONCLUSIONS: HBV genotype C is highly prevalent in Korea. Although it is a small percentage, genotype A also exists and it seems to take a more benign clinical course than genotype C. Further studies are necessitated to assess the relationship between HBV genotypes and the various aspects of the diseases' clinical course.",
author = "Lee, {Jung Min} and SangHoon Ahn and Chang, {Hye Young} and Shin, {Ji Eun} and doyoung kim and Sim, {Myoung Ki} and Hong, {Sun Pyo} and Chung, {Hyun Jae} and Kim, {Soo Ok} and KwangHyub Han and Chon, {Chae Yoon} and Moon, {Young Myoung}",
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Lee, JM, Ahn, S, Chang, HY, Shin, JE, kim, D, Sim, MK, Hong, SP, Chung, HJ, Kim, SO, Han, K, Chon, CY & Moon, YM 2004, 'Reappraisal of HBV genotypes and clinical significance in Koreans using MALDI-TOF mass spectrometry', The Korean journal of hepatology, vol. 10, no. 4, pp. 260-270.

Reappraisal of HBV genotypes and clinical significance in Koreans using MALDI-TOF mass spectrometry. / Lee, Jung Min; Ahn, SangHoon; Chang, Hye Young; Shin, Ji Eun; kim, doyoung; Sim, Myoung Ki; Hong, Sun Pyo; Chung, Hyun Jae; Kim, Soo Ok; Han, KwangHyub; Chon, Chae Yoon; Moon, Young Myoung.

In: The Korean journal of hepatology, Vol. 10, No. 4, 01.01.2004, p. 260-270.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Reappraisal of HBV genotypes and clinical significance in Koreans using MALDI-TOF mass spectrometry

AU - Lee, Jung Min

AU - Ahn, SangHoon

AU - Chang, Hye Young

AU - Shin, Ji Eun

AU - kim, doyoung

AU - Sim, Myoung Ki

AU - Hong, Sun Pyo

AU - Chung, Hyun Jae

AU - Kim, Soo Ok

AU - Han, KwangHyub

AU - Chon, Chae Yoon

AU - Moon, Young Myoung

PY - 2004/1/1

Y1 - 2004/1/1

N2 - BACKGROUND/AIMS: Recent studies have shown that the genotype of hepatitis B virus (HBV) may correlate with the disease natural history and treatment outcome. However, several of these studies used low sensitivity assays in a small number of patients, and this has precluded an accurate evaluation of Korean HBV genotypes. We analyzed Korean HBV genotypes in a large population by employing a new technology, restriction fragment mass polymorphism (RFMP) using MALDI-TOF mass spectrometry, in a sensitive and specific manner. METHODS: Between February 1995 and December 2003, a total of 475 patients with chronic HBV infection were enrolled. The assay is based on the mass measurement of oligonucleotides having genotypic variations of the S gene. Clinical features including the virologic status and disease progression were also evaluated. RESULTS: The median age of the total patients was 35.5 years. Out of 475 patients, there were 162 (34.1%) inactive carriers, 172 (36.2%) had chronic hepatitis, 77 (16.2%) had liver cirrhosis and 64 (13.5%) had hepatocellular carcinoma (HCC). There were 454 patients (95.6%) with genotype C, 4 patients (0.8%) with genotype A, 16 patients (3.4%) with the mixed A and C genotype [7 patients (1.4%) with A<C versus 9 patients (2.0%) with C>A], and 1 patient (0.2%) with B genotype. Comparing genotype A and C, genotype A patients were all inactive carriers without HCC, whereas genotype C patients included those with chronic hepatitis, liver cirrhosis and HCC. CONCLUSIONS: HBV genotype C is highly prevalent in Korea. Although it is a small percentage, genotype A also exists and it seems to take a more benign clinical course than genotype C. Further studies are necessitated to assess the relationship between HBV genotypes and the various aspects of the diseases' clinical course.

AB - BACKGROUND/AIMS: Recent studies have shown that the genotype of hepatitis B virus (HBV) may correlate with the disease natural history and treatment outcome. However, several of these studies used low sensitivity assays in a small number of patients, and this has precluded an accurate evaluation of Korean HBV genotypes. We analyzed Korean HBV genotypes in a large population by employing a new technology, restriction fragment mass polymorphism (RFMP) using MALDI-TOF mass spectrometry, in a sensitive and specific manner. METHODS: Between February 1995 and December 2003, a total of 475 patients with chronic HBV infection were enrolled. The assay is based on the mass measurement of oligonucleotides having genotypic variations of the S gene. Clinical features including the virologic status and disease progression were also evaluated. RESULTS: The median age of the total patients was 35.5 years. Out of 475 patients, there were 162 (34.1%) inactive carriers, 172 (36.2%) had chronic hepatitis, 77 (16.2%) had liver cirrhosis and 64 (13.5%) had hepatocellular carcinoma (HCC). There were 454 patients (95.6%) with genotype C, 4 patients (0.8%) with genotype A, 16 patients (3.4%) with the mixed A and C genotype [7 patients (1.4%) with A<C versus 9 patients (2.0%) with C>A], and 1 patient (0.2%) with B genotype. Comparing genotype A and C, genotype A patients were all inactive carriers without HCC, whereas genotype C patients included those with chronic hepatitis, liver cirrhosis and HCC. CONCLUSIONS: HBV genotype C is highly prevalent in Korea. Although it is a small percentage, genotype A also exists and it seems to take a more benign clinical course than genotype C. Further studies are necessitated to assess the relationship between HBV genotypes and the various aspects of the diseases' clinical course.

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