REBA HPV-ID® for efficient genotyping of human papillomavirus in clinical samples from Korean patients

Sunghyun Kim, Dongsup Lee, Sangjung Park, Tae Ue Kim, Bo Young Jeon, Kwang Hwa Park, Hyeyoung Lee

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9 Citations (Scopus)

Abstract

This study was conducted to evaluate the overall performance of a reverse blot hybridization-based assay, REBA HPV-ID® (Molecules and Diagnostics, Wonju, Korea) for genotyping human papillomaviruses (HPV). HPV Genotyping on 356 specimens examined cytologically was performed using the REBA HPV-ID®, and its results were compared with those obtained using the MyHPV DNA Chip® (Mygene, Seoul, Korea), DNA chip-based HPV genotyping assay. The results from this study showed that the positivity rate of the REBA HPV-ID® for abnormal cytological samples was higher (80.9%) than that of the MyHPV DNA chip (69.8%). In addition, the REBA HPV-ID® positivity rate with normal cytological samples was higher (64.4%) than that obtained using DNA chips (34.4%). Subsequently, sequence analysis was performed with specimens that generated conflicting test results. Sequence analysis confirmed that the specimens which were positive by REBA HPV-ID® did indeed contain HPV sequences. The results of this study suggest that the REBA HPV-ID® is a sensitive test for genotyping HPV of clinical specimens.

Original languageEnglish
Pages (from-to)1248-1253
Number of pages6
JournalJournal of Medical Virology
Volume84
Issue number8
DOIs
Publication statusPublished - 2012 Aug 1

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All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

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