REBA HPV-ID® for efficient genotyping of human papillomavirus in clinical samples from Korean patients

Sunghyun Kim, Dongsup Lee, Sangjung Park, Tae Ue Kim, Bo Young Jeon, Kwang Hwa Park, Hyeyoung Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

This study was conducted to evaluate the overall performance of a reverse blot hybridization-based assay, REBA HPV-ID® (Molecules and Diagnostics, Wonju, Korea) for genotyping human papillomaviruses (HPV). HPV Genotyping on 356 specimens examined cytologically was performed using the REBA HPV-ID®, and its results were compared with those obtained using the MyHPV DNA Chip® (Mygene, Seoul, Korea), DNA chip-based HPV genotyping assay. The results from this study showed that the positivity rate of the REBA HPV-ID® for abnormal cytological samples was higher (80.9%) than that of the MyHPV DNA chip (69.8%). In addition, the REBA HPV-ID® positivity rate with normal cytological samples was higher (64.4%) than that obtained using DNA chips (34.4%). Subsequently, sequence analysis was performed with specimens that generated conflicting test results. Sequence analysis confirmed that the specimens which were positive by REBA HPV-ID® did indeed contain HPV sequences. The results of this study suggest that the REBA HPV-ID® is a sensitive test for genotyping HPV of clinical specimens.

Original languageEnglish
Pages (from-to)1248-1253
Number of pages6
JournalJournal of Medical Virology
Volume84
Issue number8
DOIs
Publication statusPublished - 2012 Aug 1

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Oligonucleotide Array Sequence Analysis
Korea
Sequence Analysis

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

Cite this

Kim, Sunghyun ; Lee, Dongsup ; Park, Sangjung ; Kim, Tae Ue ; Jeon, Bo Young ; Park, Kwang Hwa ; Lee, Hyeyoung. / REBA HPV-ID® for efficient genotyping of human papillomavirus in clinical samples from Korean patients. In: Journal of Medical Virology. 2012 ; Vol. 84, No. 8. pp. 1248-1253.
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REBA HPV-ID® for efficient genotyping of human papillomavirus in clinical samples from Korean patients. / Kim, Sunghyun; Lee, Dongsup; Park, Sangjung; Kim, Tae Ue; Jeon, Bo Young; Park, Kwang Hwa; Lee, Hyeyoung.

In: Journal of Medical Virology, Vol. 84, No. 8, 01.08.2012, p. 1248-1253.

Research output: Contribution to journalArticle

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