Rebamipide-induced downregulation of phospholipase D inhibits inflammation and proliferation in gastric cancer cells

Dong Woo Kang, Gyesik Min, Do Yoon Park, Ki Whan Hong, Do Sik Min

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Rebamipide a gastroprotective drug, is clinically used for the treatment of gastric ulcers and gastritis, but its actions on gastric cancer are not clearly understood. Phospholipase D (PLD) is overexpressed in various types of cancer tissues and has been implicated as a critical factor in inflammation and carcinogenesis. However, whether rebamipide is involved in the regulation of PLD in gastric cancer cells is not known. In this study, we showed that rebamipide significantly suppressed the expression of both PLD1 and PLD2 at a transcriptional level in AGS and MKN-1 gastric cancer cells. Downregulation of PLD expression by rebamipide inhibited its enzymatic activity. In addition, rebamipide inhibited the transactivation of nuclear factor kappa B (NFκB), which increased PLD1 expression. Rebamipide or PLD knockdown significantly suppressed the expression of genes involved in inflammation and proliferation and inhibited the proliferation of gastric cancer cells. In conclusion, rebamipide-induced downregulation of PLD may contribute to the inhibition of inflammation and proliferation in gastric cancer.

Original languageEnglish
Pages (from-to)554-564
Number of pages11
JournalExperimental and Molecular Medicine
Volume42
Issue number8
DOIs
Publication statusPublished - 2010 Jan 1

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Phospholipase D
Stomach Neoplasms
Down-Regulation
Cells
Inflammation
NF-kappa B
Gastritis
Stomach Ulcer
rebamipide
Transcriptional Activation
Carcinogenesis
Genes
Tissue
Gene Expression
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

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Rebamipide-induced downregulation of phospholipase D inhibits inflammation and proliferation in gastric cancer cells. / Kang, Dong Woo; Min, Gyesik; Park, Do Yoon; Hong, Ki Whan; Min, Do Sik.

In: Experimental and Molecular Medicine, Vol. 42, No. 8, 01.01.2010, p. 554-564.

Research output: Contribution to journalArticle

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