Az osteopontin csökkent kifejezodésének újabb bizonyítékai mola hydatidosában

Translated title of the contribution: Recent evidences for lower expression of osteopontin in hydatidiform mole

József Bátorfi, György Végh, Kim Jae-Hoon, Samuel C. Mok, Ross S. Berkowitz, József Doszpod, Vilmos Fülöp, Imre Cseh

Research output: Contribution to journalArticle

Abstract

Osteopontin (OPN) is a protein of the extracellular matrix, which plays a role in the tumorgenesis of several types of malignant tumors. In a collaborative study of Hungarian National Health Center in Budapest and the Harvard Medical School in Boston, USA, our workgroup has previously reported a comparison of OPN expression between normal human placenta and molar tissues. Using Laser Caption Microdissection, m-RNA extraction, real-time PCR and gel-electrophoresis methods, we have found a significant down-regulation of OPN m-RNA in hydatidiform mole. In this study we analyzed the OPN protein appearance in mole and normal trophoblast in order to confirm our previous findings. Immunohistochemical examinations were performed on 13 complete moles, 2 partial moles and 9 normal placentas from comparable gestational ages. Our results verified the definite down-regulation of OPN protein in mole. Although further examinations are required to identify the exact place of OPN in the biochemical background of molar pregnancies, our findings suggest that the lower expression of OPN may play an important role in the pathogenesis of gestational trophoblastic diseases.

Original languageHungarian
Pages (from-to)351-355
Number of pages5
JournalMagyar Noorvosok Lapja
Volume66
Issue number6
Publication statusPublished - 2003 Dec 9

Fingerprint

Hydatidiform Mole
Osteopontin
Placenta
Down-Regulation
RNA
Gestational Trophoblastic Disease
Microdissection
Extracellular Matrix Proteins
Trophoblasts
Medical Schools
Gestational Age
Electrophoresis
Real-Time Polymerase Chain Reaction
Proteins
Lasers
Gels
Health

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

Bátorfi, J., Végh, G., Jae-Hoon, K., Mok, S. C., Berkowitz, R. S., Doszpod, J., ... Cseh, I. (2003). Az osteopontin csökkent kifejezodésének újabb bizonyítékai mola hydatidosában. Magyar Noorvosok Lapja, 66(6), 351-355.
Bátorfi, József ; Végh, György ; Jae-Hoon, Kim ; Mok, Samuel C. ; Berkowitz, Ross S. ; Doszpod, József ; Fülöp, Vilmos ; Cseh, Imre. / Az osteopontin csökkent kifejezodésének újabb bizonyítékai mola hydatidosában. In: Magyar Noorvosok Lapja. 2003 ; Vol. 66, No. 6. pp. 351-355.
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abstract = "Osteopontin (OPN) is a protein of the extracellular matrix, which plays a role in the tumorgenesis of several types of malignant tumors. In a collaborative study of Hungarian National Health Center in Budapest and the Harvard Medical School in Boston, USA, our workgroup has previously reported a comparison of OPN expression between normal human placenta and molar tissues. Using Laser Caption Microdissection, m-RNA extraction, real-time PCR and gel-electrophoresis methods, we have found a significant down-regulation of OPN m-RNA in hydatidiform mole. In this study we analyzed the OPN protein appearance in mole and normal trophoblast in order to confirm our previous findings. Immunohistochemical examinations were performed on 13 complete moles, 2 partial moles and 9 normal placentas from comparable gestational ages. Our results verified the definite down-regulation of OPN protein in mole. Although further examinations are required to identify the exact place of OPN in the biochemical background of molar pregnancies, our findings suggest that the lower expression of OPN may play an important role in the pathogenesis of gestational trophoblastic diseases.",
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Bátorfi, J, Végh, G, Jae-Hoon, K, Mok, SC, Berkowitz, RS, Doszpod, J, Fülöp, V & Cseh, I 2003, 'Az osteopontin csökkent kifejezodésének újabb bizonyítékai mola hydatidosában', Magyar Noorvosok Lapja, vol. 66, no. 6, pp. 351-355.

Az osteopontin csökkent kifejezodésének újabb bizonyítékai mola hydatidosában. / Bátorfi, József; Végh, György; Jae-Hoon, Kim; Mok, Samuel C.; Berkowitz, Ross S.; Doszpod, József; Fülöp, Vilmos; Cseh, Imre.

In: Magyar Noorvosok Lapja, Vol. 66, No. 6, 09.12.2003, p. 351-355.

Research output: Contribution to journalArticle

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T1 - Az osteopontin csökkent kifejezodésének újabb bizonyítékai mola hydatidosában

AU - Bátorfi, József

AU - Végh, György

AU - Jae-Hoon, Kim

AU - Mok, Samuel C.

AU - Berkowitz, Ross S.

AU - Doszpod, József

AU - Fülöp, Vilmos

AU - Cseh, Imre

PY - 2003/12/9

Y1 - 2003/12/9

N2 - Osteopontin (OPN) is a protein of the extracellular matrix, which plays a role in the tumorgenesis of several types of malignant tumors. In a collaborative study of Hungarian National Health Center in Budapest and the Harvard Medical School in Boston, USA, our workgroup has previously reported a comparison of OPN expression between normal human placenta and molar tissues. Using Laser Caption Microdissection, m-RNA extraction, real-time PCR and gel-electrophoresis methods, we have found a significant down-regulation of OPN m-RNA in hydatidiform mole. In this study we analyzed the OPN protein appearance in mole and normal trophoblast in order to confirm our previous findings. Immunohistochemical examinations were performed on 13 complete moles, 2 partial moles and 9 normal placentas from comparable gestational ages. Our results verified the definite down-regulation of OPN protein in mole. Although further examinations are required to identify the exact place of OPN in the biochemical background of molar pregnancies, our findings suggest that the lower expression of OPN may play an important role in the pathogenesis of gestational trophoblastic diseases.

AB - Osteopontin (OPN) is a protein of the extracellular matrix, which plays a role in the tumorgenesis of several types of malignant tumors. In a collaborative study of Hungarian National Health Center in Budapest and the Harvard Medical School in Boston, USA, our workgroup has previously reported a comparison of OPN expression between normal human placenta and molar tissues. Using Laser Caption Microdissection, m-RNA extraction, real-time PCR and gel-electrophoresis methods, we have found a significant down-regulation of OPN m-RNA in hydatidiform mole. In this study we analyzed the OPN protein appearance in mole and normal trophoblast in order to confirm our previous findings. Immunohistochemical examinations were performed on 13 complete moles, 2 partial moles and 9 normal placentas from comparable gestational ages. Our results verified the definite down-regulation of OPN protein in mole. Although further examinations are required to identify the exact place of OPN in the biochemical background of molar pregnancies, our findings suggest that the lower expression of OPN may play an important role in the pathogenesis of gestational trophoblastic diseases.

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Bátorfi J, Végh G, Jae-Hoon K, Mok SC, Berkowitz RS, Doszpod J et al. Az osteopontin csökkent kifejezodésének újabb bizonyítékai mola hydatidosában. Magyar Noorvosok Lapja. 2003 Dec 9;66(6):351-355.