Abstract
Deregulation of the cell cycle by overexpression of G1 cyclins, cyclin E and cyclin D1 genes, has been demonstrated to be a prerequisite for the development of human cancer. Recently, cyclin E is proposed to be sufficient for the progression of the G1 cell cycle without cyclin D1. Here we show that the proposed model system was specifically present in human hepatocellular carcinoma (HCC) unlike other human cancers. Of 31 HCC tissues analyzed, 21 (67.7%) exhibited an overexpression of cyclin E protein. In contrast to cyclin E gene expression, cyclin D1 expression was strongly downregulated in 19 (61.2%) HCCs. Interestingly, 65% of HCC tissues with overexpression of the cyclin E gene exhibited downregulation of cyclin D1, suggesting reciprocal deregulation of these cyclins in the G1 progression of the cell cycle. Southern blot analysis proved the amplification of cyclin E gene in HCC with a high level of overexpression. The present findings suggest that the reciprocal deregulation of cyclin E lacking cyclin D1 expression might play a role in G1 progression and the development of HCC.
| Original language | English |
|---|---|
| Pages (from-to) | 57-63 |
| Number of pages | 7 |
| Journal | Cancer Letters |
| Volume | 168 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2001 Jul 10 |
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All Science Journal Classification (ASJC) codes
- Oncology
- Cancer Research
Cite this
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Reciprocal expressions of cyclin E and cyclin D1 in hepatocellular carcinoma. / Jung, Yu Jin; Lee, Kee Ho; Choi, Dong Wook; Han, Chul Ju; Jeong, Sook Hyang; Kim, Keun Cheol; Oh, Jong Won; Park, Taek Kyu; Kim, Chang Min.
In: Cancer Letters, Vol. 168, No. 1, 10.07.2001, p. 57-63.Research output: Contribution to journal › Article
TY - JOUR
T1 - Reciprocal expressions of cyclin E and cyclin D1 in hepatocellular carcinoma
AU - Jung, Yu Jin
AU - Lee, Kee Ho
AU - Choi, Dong Wook
AU - Han, Chul Ju
AU - Jeong, Sook Hyang
AU - Kim, Keun Cheol
AU - Oh, Jong Won
AU - Park, Taek Kyu
AU - Kim, Chang Min
PY - 2001/7/10
Y1 - 2001/7/10
N2 - Deregulation of the cell cycle by overexpression of G1 cyclins, cyclin E and cyclin D1 genes, has been demonstrated to be a prerequisite for the development of human cancer. Recently, cyclin E is proposed to be sufficient for the progression of the G1 cell cycle without cyclin D1. Here we show that the proposed model system was specifically present in human hepatocellular carcinoma (HCC) unlike other human cancers. Of 31 HCC tissues analyzed, 21 (67.7%) exhibited an overexpression of cyclin E protein. In contrast to cyclin E gene expression, cyclin D1 expression was strongly downregulated in 19 (61.2%) HCCs. Interestingly, 65% of HCC tissues with overexpression of the cyclin E gene exhibited downregulation of cyclin D1, suggesting reciprocal deregulation of these cyclins in the G1 progression of the cell cycle. Southern blot analysis proved the amplification of cyclin E gene in HCC with a high level of overexpression. The present findings suggest that the reciprocal deregulation of cyclin E lacking cyclin D1 expression might play a role in G1 progression and the development of HCC.
AB - Deregulation of the cell cycle by overexpression of G1 cyclins, cyclin E and cyclin D1 genes, has been demonstrated to be a prerequisite for the development of human cancer. Recently, cyclin E is proposed to be sufficient for the progression of the G1 cell cycle without cyclin D1. Here we show that the proposed model system was specifically present in human hepatocellular carcinoma (HCC) unlike other human cancers. Of 31 HCC tissues analyzed, 21 (67.7%) exhibited an overexpression of cyclin E protein. In contrast to cyclin E gene expression, cyclin D1 expression was strongly downregulated in 19 (61.2%) HCCs. Interestingly, 65% of HCC tissues with overexpression of the cyclin E gene exhibited downregulation of cyclin D1, suggesting reciprocal deregulation of these cyclins in the G1 progression of the cell cycle. Southern blot analysis proved the amplification of cyclin E gene in HCC with a high level of overexpression. The present findings suggest that the reciprocal deregulation of cyclin E lacking cyclin D1 expression might play a role in G1 progression and the development of HCC.
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UR - http://www.scopus.com/inward/citedby.url?scp=0035838593&partnerID=8YFLogxK
U2 - 10.1016/S0304-3835(01)00403-7
DO - 10.1016/S0304-3835(01)00403-7
M3 - Article
C2 - 11368878
AN - SCOPUS:0035838593
VL - 168
SP - 57
EP - 63
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 1
ER -