Reciprocal interactions of Fgf10/Fgfr2b modulate the mouse tongue epithelial differentiation

Wern Joo Sohn, Hye In Jung, Min A. Choi, Jin Hyun Han, Gi Jeong Gwon, Hitoshi Yamamoto, Sanggyu Lee, Zae Young Ryoo, Eui Kyun Park, Hong In Shin, Hansung Jung, Jae Young Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The molecular mechanisms for epithelial differentiation have been studied by observing skin development in embryogenesis, but the early signaling modulations involved in tongue epithelial differentiation are not completely understood. Based on the gene expression patterns of the Fgf signaling molecules and previous results from Fgf10 and Fgfr2b knockout mice, it was hypothesized that there would be fundamental signaling interactions through the epithelial Fgfr2b and its mesenchymal ligand Fgf10 to regulate tongue epithelium differentiation. To elucidate these reciprocal interactions in tongue epithelial differentiation, this study employed an in vitro tongue organ culture system with antisense-oligodeoxynucleotides (AS-ODNs) and recombinant protein-soaked bead implantation for the loss-of-function and gain-of-function studies. Functional analysis of Fgf signaling revealed precise reciprocal interactions, which showed that mesenchymal Fgf10 rather than Fgf7 modulates tongue epithelial differentiation via Fgfr2b in a temporal- and spatial-specific manner.

Original languageEnglish
Pages (from-to)265-273
Number of pages9
JournalCell and Tissue Research
Volume345
Issue number2
DOIs
Publication statusPublished - 2011 Aug 1

Fingerprint

Tongue
Oligodeoxyribonucleotides
Organ Culture Techniques
Recombinant Proteins
Knockout Mice
Embryonic Development
Epithelium
Ligands
Gene Expression
Skin

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Cite this

Sohn, W. J., Jung, H. I., Choi, M. A., Han, J. H., Gwon, G. J., Yamamoto, H., ... Kim, J. Y. (2011). Reciprocal interactions of Fgf10/Fgfr2b modulate the mouse tongue epithelial differentiation. Cell and Tissue Research, 345(2), 265-273. https://doi.org/10.1007/s00441-011-1204-8
Sohn, Wern Joo ; Jung, Hye In ; Choi, Min A. ; Han, Jin Hyun ; Gwon, Gi Jeong ; Yamamoto, Hitoshi ; Lee, Sanggyu ; Ryoo, Zae Young ; Park, Eui Kyun ; Shin, Hong In ; Jung, Hansung ; Kim, Jae Young. / Reciprocal interactions of Fgf10/Fgfr2b modulate the mouse tongue epithelial differentiation. In: Cell and Tissue Research. 2011 ; Vol. 345, No. 2. pp. 265-273.
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Sohn, WJ, Jung, HI, Choi, MA, Han, JH, Gwon, GJ, Yamamoto, H, Lee, S, Ryoo, ZY, Park, EK, Shin, HI, Jung, H & Kim, JY 2011, 'Reciprocal interactions of Fgf10/Fgfr2b modulate the mouse tongue epithelial differentiation', Cell and Tissue Research, vol. 345, no. 2, pp. 265-273. https://doi.org/10.1007/s00441-011-1204-8

Reciprocal interactions of Fgf10/Fgfr2b modulate the mouse tongue epithelial differentiation. / Sohn, Wern Joo; Jung, Hye In; Choi, Min A.; Han, Jin Hyun; Gwon, Gi Jeong; Yamamoto, Hitoshi; Lee, Sanggyu; Ryoo, Zae Young; Park, Eui Kyun; Shin, Hong In; Jung, Hansung; Kim, Jae Young.

In: Cell and Tissue Research, Vol. 345, No. 2, 01.08.2011, p. 265-273.

Research output: Contribution to journalArticle

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