Recognition of left-handed Z-DNA of short unmodified oligonucleotides under physiological ionic strength conditions

Alessandro D'Urso, Jung Kyu Choi, Murtaza Shabbir-Hussain, Fidelis N. Ngwa, Maria I. Lambousis, Roberto Purrello, Milan Balaz

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The left-handed Z-DNA form of the short unmodified alternating guanine-cytosine oligonucleotides, 5'-(dGdC)24 and 5'-(dGdC)18, was selectively detected under physiological ionic strength and pH conditions using the anionic nickel(II) porphyrin, NiTPPS. No spectroscopic signal was observed for NiTPPS with any right-handed oligonucleotides under identical conditions. The 48mer 5'-(dGdC)24 Z-form was detected at concentrations as low as 100 nM. The binding of NiTPPS to the B- and Z-oligonucleotides was studied quantitatively by UV-vis absorption and circular dichroism spectroscopies. NiTPPS was found to be a universal DNA binder, with binding affinity and geometry depending on the ionic composition of the solution, rather than on the DNA helical twist. This is the first example of a successful spectroscopic detection of the Z-DNA of short unmodified oligonucleotides under physiological pH and ionic strength conditions.

Original languageEnglish
Pages (from-to)329-332
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume397
Issue number2
DOIs
Publication statusPublished - 2010 Jun 25

Fingerprint

Z-Form DNA
Ionic strength
Oligonucleotides
Osmolar Concentration
Circular dichroism spectroscopy
Cytosine
DNA
Porphyrins
Guanine
Circular Dichroism
Nickel
Binders
Spectrum Analysis
Geometry
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

D'Urso, Alessandro ; Choi, Jung Kyu ; Shabbir-Hussain, Murtaza ; Ngwa, Fidelis N. ; Lambousis, Maria I. ; Purrello, Roberto ; Balaz, Milan. / Recognition of left-handed Z-DNA of short unmodified oligonucleotides under physiological ionic strength conditions. In: Biochemical and Biophysical Research Communications. 2010 ; Vol. 397, No. 2. pp. 329-332.
@article{8cd2e2fd5be6468ba3520e3c1d600b6f,
title = "Recognition of left-handed Z-DNA of short unmodified oligonucleotides under physiological ionic strength conditions",
abstract = "The left-handed Z-DNA form of the short unmodified alternating guanine-cytosine oligonucleotides, 5'-(dGdC)24 and 5'-(dGdC)18, was selectively detected under physiological ionic strength and pH conditions using the anionic nickel(II) porphyrin, NiTPPS. No spectroscopic signal was observed for NiTPPS with any right-handed oligonucleotides under identical conditions. The 48mer 5'-(dGdC)24 Z-form was detected at concentrations as low as 100 nM. The binding of NiTPPS to the B- and Z-oligonucleotides was studied quantitatively by UV-vis absorption and circular dichroism spectroscopies. NiTPPS was found to be a universal DNA binder, with binding affinity and geometry depending on the ionic composition of the solution, rather than on the DNA helical twist. This is the first example of a successful spectroscopic detection of the Z-DNA of short unmodified oligonucleotides under physiological pH and ionic strength conditions.",
author = "Alessandro D'Urso and Choi, {Jung Kyu} and Murtaza Shabbir-Hussain and Ngwa, {Fidelis N.} and Lambousis, {Maria I.} and Roberto Purrello and Milan Balaz",
year = "2010",
month = "6",
day = "25",
doi = "10.1016/j.bbrc.2010.05.119",
language = "English",
volume = "397",
pages = "329--332",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

Recognition of left-handed Z-DNA of short unmodified oligonucleotides under physiological ionic strength conditions. / D'Urso, Alessandro; Choi, Jung Kyu; Shabbir-Hussain, Murtaza; Ngwa, Fidelis N.; Lambousis, Maria I.; Purrello, Roberto; Balaz, Milan.

In: Biochemical and Biophysical Research Communications, Vol. 397, No. 2, 25.06.2010, p. 329-332.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Recognition of left-handed Z-DNA of short unmodified oligonucleotides under physiological ionic strength conditions

AU - D'Urso, Alessandro

AU - Choi, Jung Kyu

AU - Shabbir-Hussain, Murtaza

AU - Ngwa, Fidelis N.

AU - Lambousis, Maria I.

AU - Purrello, Roberto

AU - Balaz, Milan

PY - 2010/6/25

Y1 - 2010/6/25

N2 - The left-handed Z-DNA form of the short unmodified alternating guanine-cytosine oligonucleotides, 5'-(dGdC)24 and 5'-(dGdC)18, was selectively detected under physiological ionic strength and pH conditions using the anionic nickel(II) porphyrin, NiTPPS. No spectroscopic signal was observed for NiTPPS with any right-handed oligonucleotides under identical conditions. The 48mer 5'-(dGdC)24 Z-form was detected at concentrations as low as 100 nM. The binding of NiTPPS to the B- and Z-oligonucleotides was studied quantitatively by UV-vis absorption and circular dichroism spectroscopies. NiTPPS was found to be a universal DNA binder, with binding affinity and geometry depending on the ionic composition of the solution, rather than on the DNA helical twist. This is the first example of a successful spectroscopic detection of the Z-DNA of short unmodified oligonucleotides under physiological pH and ionic strength conditions.

AB - The left-handed Z-DNA form of the short unmodified alternating guanine-cytosine oligonucleotides, 5'-(dGdC)24 and 5'-(dGdC)18, was selectively detected under physiological ionic strength and pH conditions using the anionic nickel(II) porphyrin, NiTPPS. No spectroscopic signal was observed for NiTPPS with any right-handed oligonucleotides under identical conditions. The 48mer 5'-(dGdC)24 Z-form was detected at concentrations as low as 100 nM. The binding of NiTPPS to the B- and Z-oligonucleotides was studied quantitatively by UV-vis absorption and circular dichroism spectroscopies. NiTPPS was found to be a universal DNA binder, with binding affinity and geometry depending on the ionic composition of the solution, rather than on the DNA helical twist. This is the first example of a successful spectroscopic detection of the Z-DNA of short unmodified oligonucleotides under physiological pH and ionic strength conditions.

UR - http://www.scopus.com/inward/record.url?scp=77955504375&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955504375&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2010.05.119

DO - 10.1016/j.bbrc.2010.05.119

M3 - Article

C2 - 20510880

AN - SCOPUS:77955504375

VL - 397

SP - 329

EP - 332

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -