Recognition of left-handed Z-DNA of short unmodified oligonucleotides under physiological ionic strength conditions

Alessandro D'Urso, Jung Kyu Choi, Murtaza Shabbir-Hussain, Fidelis N. Ngwa, Maria I. Lambousis, Roberto Purrello, Milan Balaz

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The left-handed Z-DNA form of the short unmodified alternating guanine-cytosine oligonucleotides, 5'-(dGdC)24 and 5'-(dGdC)18, was selectively detected under physiological ionic strength and pH conditions using the anionic nickel(II) porphyrin, NiTPPS. No spectroscopic signal was observed for NiTPPS with any right-handed oligonucleotides under identical conditions. The 48mer 5'-(dGdC)24 Z-form was detected at concentrations as low as 100 nM. The binding of NiTPPS to the B- and Z-oligonucleotides was studied quantitatively by UV-vis absorption and circular dichroism spectroscopies. NiTPPS was found to be a universal DNA binder, with binding affinity and geometry depending on the ionic composition of the solution, rather than on the DNA helical twist. This is the first example of a successful spectroscopic detection of the Z-DNA of short unmodified oligonucleotides under physiological pH and ionic strength conditions.

Original languageEnglish
Pages (from-to)329-332
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume397
Issue number2
DOIs
Publication statusPublished - 2010 Jun 25

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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