Abstract
Due to the lack of an effective prophylactic intervention and diagnosis, human liver fluke Clonorchis sinensis continues to afflict a large human population, causing a chronic inflammatory bile duct disease. With an aim to identify target antigens for sensitive serodiagnosis, adenylate kinase 3 of C. sinensis (CsAK3) was successfully expressed in soluble form in Escherichia coli by fusion to an RNA-interacting domain derived from human Lys-tRNA synthetase and purified by Ni2+-affinity chromatography. Anti-CsAK3 serum was raised by immunization of mice, and Western blotting confirmed that CsAK3 was expressed in adult-stage C. sinensis. Histochemical analysis showed that CsAK3 was localized to the subtegumental tissue of C. sinensis and was excreted into the bile duct of the host. When tested against sera from various parasite-infected patients by enzyme-linked immunosorbent assay, the recombinant CsAK3 elicited a specific response to C. sinensis-infected sera. The results suggest that CsAK3, either alone or in combination with other antigens, could be used for improving the clinical diagnosis of clonorchiasis.
| Original language | English |
|---|---|
| Pages (from-to) | 1531-1539 |
| Number of pages | 9 |
| Journal | Parasitology |
| Volume | 145 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2018 Oct 1 |
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All Science Journal Classification (ASJC) codes
- Parasitology
- Animal Science and Zoology
- Infectious Diseases
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Recombinant adenylate kinase 3 from liver fluke Clonorchis sinensis for histochemical analysis and serodiagnosis of clonorchiasis. / Kwon, Soon Bin; Kim, Paul; Woo, Hae Sun; Kim, Tae Yun; Kim, Ju Yeong; Lee, Hye Min; Jang, Yun Soo; Kim, Eun Min; Yong, Taisoon; Seong, Baik Lin.
In: Parasitology, Vol. 145, No. 12, 01.10.2018, p. 1531-1539.Research output: Contribution to journal › Article
TY - JOUR
T1 - Recombinant adenylate kinase 3 from liver fluke Clonorchis sinensis for histochemical analysis and serodiagnosis of clonorchiasis
AU - Kwon, Soon Bin
AU - Kim, Paul
AU - Woo, Hae Sun
AU - Kim, Tae Yun
AU - Kim, Ju Yeong
AU - Lee, Hye Min
AU - Jang, Yun Soo
AU - Kim, Eun Min
AU - Yong, Taisoon
AU - Seong, Baik Lin
PY - 2018/10/1
Y1 - 2018/10/1
N2 - Due to the lack of an effective prophylactic intervention and diagnosis, human liver fluke Clonorchis sinensis continues to afflict a large human population, causing a chronic inflammatory bile duct disease. With an aim to identify target antigens for sensitive serodiagnosis, adenylate kinase 3 of C. sinensis (CsAK3) was successfully expressed in soluble form in Escherichia coli by fusion to an RNA-interacting domain derived from human Lys-tRNA synthetase and purified by Ni2+-affinity chromatography. Anti-CsAK3 serum was raised by immunization of mice, and Western blotting confirmed that CsAK3 was expressed in adult-stage C. sinensis. Histochemical analysis showed that CsAK3 was localized to the subtegumental tissue of C. sinensis and was excreted into the bile duct of the host. When tested against sera from various parasite-infected patients by enzyme-linked immunosorbent assay, the recombinant CsAK3 elicited a specific response to C. sinensis-infected sera. The results suggest that CsAK3, either alone or in combination with other antigens, could be used for improving the clinical diagnosis of clonorchiasis.
AB - Due to the lack of an effective prophylactic intervention and diagnosis, human liver fluke Clonorchis sinensis continues to afflict a large human population, causing a chronic inflammatory bile duct disease. With an aim to identify target antigens for sensitive serodiagnosis, adenylate kinase 3 of C. sinensis (CsAK3) was successfully expressed in soluble form in Escherichia coli by fusion to an RNA-interacting domain derived from human Lys-tRNA synthetase and purified by Ni2+-affinity chromatography. Anti-CsAK3 serum was raised by immunization of mice, and Western blotting confirmed that CsAK3 was expressed in adult-stage C. sinensis. Histochemical analysis showed that CsAK3 was localized to the subtegumental tissue of C. sinensis and was excreted into the bile duct of the host. When tested against sera from various parasite-infected patients by enzyme-linked immunosorbent assay, the recombinant CsAK3 elicited a specific response to C. sinensis-infected sera. The results suggest that CsAK3, either alone or in combination with other antigens, could be used for improving the clinical diagnosis of clonorchiasis.
UR - http://www.scopus.com/inward/record.url?scp=85044452062&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85044452062&partnerID=8YFLogxK
U2 - 10.1017/S0031182018000434
DO - 10.1017/S0031182018000434
M3 - Article
C2 - 29580315
AN - SCOPUS:85044452062
VL - 145
SP - 1531
EP - 1539
JO - Parasitology
JF - Parasitology
SN - 0031-1820
IS - 12
ER -