Recombinant adenylate kinase 3 from liver fluke Clonorchis sinensis for histochemical analysis and serodiagnosis of clonorchiasis

Soon Bin Kwon, Paul Kim, Hae Sun Woo, Tae Yun Kim, Ju Yeong Kim, Hye Min Lee, Yun Soo Jang, Eun Min Kim, Taisoon Yong, Baik Lin Seong

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Due to the lack of an effective prophylactic intervention and diagnosis, human liver fluke Clonorchis sinensis continues to afflict a large human population, causing a chronic inflammatory bile duct disease. With an aim to identify target antigens for sensitive serodiagnosis, adenylate kinase 3 of C. sinensis (CsAK3) was successfully expressed in soluble form in Escherichia coli by fusion to an RNA-interacting domain derived from human Lys-tRNA synthetase and purified by Ni2+-affinity chromatography. Anti-CsAK3 serum was raised by immunization of mice, and Western blotting confirmed that CsAK3 was expressed in adult-stage C. sinensis. Histochemical analysis showed that CsAK3 was localized to the subtegumental tissue of C. sinensis and was excreted into the bile duct of the host. When tested against sera from various parasite-infected patients by enzyme-linked immunosorbent assay, the recombinant CsAK3 elicited a specific response to C. sinensis-infected sera. The results suggest that CsAK3, either alone or in combination with other antigens, could be used for improving the clinical diagnosis of clonorchiasis.

Original languageEnglish
Pages (from-to)1531-1539
Number of pages9
JournalParasitology
Volume145
Issue number12
DOIs
Publication statusPublished - 2018 Oct 1

Fingerprint

Clonorchis sinensis
Clonorchiasis
adenylate kinase
Adenylate Kinase
liver flukes
Fasciola hepatica
serodiagnosis
Serologic Tests
blood serum
biliary tract diseases
Bile Duct Diseases
Serum
antigens
Antigens
Amino Acyl-tRNA Synthetases
bile ducts
affinity chromatography
Bile Ducts
Affinity Chromatography
ligases

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Animal Science and Zoology
  • Infectious Diseases

Cite this

Kwon, Soon Bin ; Kim, Paul ; Woo, Hae Sun ; Kim, Tae Yun ; Kim, Ju Yeong ; Lee, Hye Min ; Jang, Yun Soo ; Kim, Eun Min ; Yong, Taisoon ; Seong, Baik Lin. / Recombinant adenylate kinase 3 from liver fluke Clonorchis sinensis for histochemical analysis and serodiagnosis of clonorchiasis. In: Parasitology. 2018 ; Vol. 145, No. 12. pp. 1531-1539.
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Recombinant adenylate kinase 3 from liver fluke Clonorchis sinensis for histochemical analysis and serodiagnosis of clonorchiasis. / Kwon, Soon Bin; Kim, Paul; Woo, Hae Sun; Kim, Tae Yun; Kim, Ju Yeong; Lee, Hye Min; Jang, Yun Soo; Kim, Eun Min; Yong, Taisoon; Seong, Baik Lin.

In: Parasitology, Vol. 145, No. 12, 01.10.2018, p. 1531-1539.

Research output: Contribution to journalArticle

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