Reconstruction of the immunoaffinity layer of SPR biosensor by using proteolytic enzyme

Usually, SPR biosensor has been applied for the detection of a target analyte in a sample by using the highly specific antigen-antibody interaction. For the specific binding of the target analyte in a sample, suitable antibodies are usually immobilized on the sensor surface, which is called 'immunoaffinity (IA) layer'. After repeated sample detection and regeneration processes, the activity of IA layer is damaged and thrown away. In this work, a reconstruction method of IA is presented by using a proteolytic enzyme. By analysis of the amount of immobilized antibodies to the reconstructed IA layer as well as the activity of reconstructed IA layer of the reconstructed IA layers, the feasibility of reconstruction method was demonstrated.