Recording of elapsed time and temporal information about biological events using Cas9

Jihye Park; Jung Min Lim; Inkyung Jung; Seok Jae Heo; Jinman Park; Yoojin Chang; Hui Kwon Kim; Dongmin Jung; Ji Hea Yu; Seonwoo Min; Sungroh Yoon; Sung Rae Cho; Taeyoung Park; Hyongbum Henry Kim

doi:10.1016/j.cell.2021.01.014

Recording of elapsed time and temporal information about biological events using Cas9

Jihye Park, Jung Min Lim, Inkyung Jung, Seok Jae Heo, Jinman Park, Yoojin Chang, Hui Kwon Kim, Dongmin Jung, Ji Hea Yu, Seonwoo Min, Sungroh Yoon, Sung Rae Cho, Taeyoung Park, Hyongbum Henry Kim

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Indel generation by Cas9 and guide RNA can be used to record time and temporal information about biological events.

Original language	English
Pages (from-to)	1047-1063.e23
Journal	Cell
Volume	184
Issue number	4
DOIs	https://doi.org/10.1016/j.cell.2021.01.014
Publication status	Published - 2021 Feb 18

Bibliographical note

Funding Information:
We are grateful to Bukweon Kim (Department of Computational Science and Engineering, Yonsei University) and Sungchul Lee (Department of Mathematics, Yonsei University) for their assistance with computational analyses. We thank Minyoung Lee for proofreading. This work was supported by the Samsung Research Funding & Incubation Center of Samsung Electronics under project number SRFC-MA1701-06. Jihye Park performed experiments with critical help from J.M.L. and Y.C. Jihye Park, J.M.L, Jinman Park, I.J. S.-J.H. D.J. T.P. and H.H.K. analyzed data. H.K.K. helped data analyses and provided technical comments on experiments. I.J. and S.-J.H. performed statistical analyses and computational simulations. S.M. and S.Y. conducted Tree SHAP analyses. H.H.K. led data analysis and supervised the project. T.P. and I.J. provided important comments on statistical analysis. J.H.Y. and S.-R.C. performed western blotting experiments. H.H.K. conceived and designed the study. H.H.K. wrote the manuscript with input from all authors. Jihye Park. J.M.L, Jinman Park. S.-J.H, I.J. and H.H.K. wrote the Methods section of the manuscript. Yonsei University has filed a patent (PCT/KR2019/015372) based on this work in which Jihye Park and H.H.K. are the co-inventors.

Funding Information:
We are grateful to Bukweon Kim (Department of Computational Science and Engineering, Yonsei University) and Sungchul Lee (Department of Mathematics, Yonsei University) for their assistance with computational analyses. We thank Minyoung Lee for proofreading. This work was supported by the Samsung Research Funding & Incubation Center of Samsung Electronics under project number SRFC-MA1701-06 .

Publisher Copyright:
© 2021 Elsevier Inc.

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/j.cell.2021.01.014

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@article{ffadb5084edb4ff5a80b623a1bb18f93,

title = "Recording of elapsed time and temporal information about biological events using Cas9",

abstract = "Indel generation by Cas9 and guide RNA can be used to record time and temporal information about biological events.",

author = "Jihye Park and Lim, {Jung Min} and Inkyung Jung and Heo, {Seok Jae} and Jinman Park and Yoojin Chang and Kim, {Hui Kwon} and Dongmin Jung and Yu, {Ji Hea} and Seonwoo Min and Sungroh Yoon and Cho, {Sung Rae} and Taeyoung Park and Kim, {Hyongbum Henry}",

note = "Funding Information: We are grateful to Bukweon Kim (Department of Computational Science and Engineering, Yonsei University) and Sungchul Lee (Department of Mathematics, Yonsei University) for their assistance with computational analyses. We thank Minyoung Lee for proofreading. This work was supported by the Samsung Research Funding & Incubation Center of Samsung Electronics under project number SRFC-MA1701-06. Jihye Park performed experiments with critical help from J.M.L. and Y.C. Jihye Park, J.M.L, Jinman Park, I.J. S.-J.H. D.J. T.P. and H.H.K. analyzed data. H.K.K. helped data analyses and provided technical comments on experiments. I.J. and S.-J.H. performed statistical analyses and computational simulations. S.M. and S.Y. conducted Tree SHAP analyses. H.H.K. led data analysis and supervised the project. T.P. and I.J. provided important comments on statistical analysis. J.H.Y. and S.-R.C. performed western blotting experiments. H.H.K. conceived and designed the study. H.H.K. wrote the manuscript with input from all authors. Jihye Park. J.M.L, Jinman Park. S.-J.H, I.J. and H.H.K. wrote the Methods section of the manuscript. Yonsei University has filed a patent (PCT/KR2019/015372) based on this work in which Jihye Park and H.H.K. are the co-inventors. Funding Information: We are grateful to Bukweon Kim (Department of Computational Science and Engineering, Yonsei University) and Sungchul Lee (Department of Mathematics, Yonsei University) for their assistance with computational analyses. We thank Minyoung Lee for proofreading. This work was supported by the Samsung Research Funding & Incubation Center of Samsung Electronics under project number SRFC-MA1701-06 . Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",

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AU - Lim, Jung Min

AU - Jung, Inkyung

AU - Heo, Seok Jae

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AU - Chang, Yoojin

AU - Kim, Hui Kwon

AU - Jung, Dongmin

AU - Yu, Ji Hea

AU - Min, Seonwoo

AU - Yoon, Sungroh

AU - Cho, Sung Rae

AU - Park, Taeyoung

AU - Kim, Hyongbum Henry

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Recording of elapsed time and temporal information about biological events using Cas9

Abstract

Bibliographical note

All Science Journal Classification (ASJC) codes

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