Abstract
A new combined method including the enzymatic hydrolysis of β-cyclodextrin (β-CD) and solvent extraction of cholesterol from the hydrolyzed mixture was developed to recover cholesterol from a β-CD-cholesterol complex prepared from dairy products, such as cream, milk, and cheese. Cyclomaltodextrinase (cyclomaltodextrin dextrin hydrolase, EC 3.2.1.54, CDase) prepared from alkalophilic Bacillus sp. KJ 133 hydrolyzed the β-CD of the β-CD-cholesterol complex, and then, free cholesterol was efficiently extracted from the hydrolyzed mixture by a nonpolar solvent such as ethyl acetate. To increase the stability of free CDase, immobilized CDase was developed using sodium alginate as a carrier. The immobilized CDase showed a high recovery yield of cholesterol in a time-dependent manner compared to the free CDase. A gas chromatography analysis showed that more than 70% of cholesterol was recovered from the β-CD-cholesterol complex of cream by the immobilized CDase, whereas only 3% and 29% of cholesterol were recovered when the solvent extraction and free CDase treatment were used, respectively. The cholesterol recovered can be used as a raw material for steroid synthesis. Furthermore, this method can be an efficient way to recover cholesterol or other organic compounds that are bound in a β-CD-cholesterol or -organic compound complex.
| Original language | English |
|---|---|
| Pages (from-to) | 712-715 |
| Number of pages | 4 |
| Journal | Journal of Microbiology and Biotechnology |
| Volume | 11 |
| Issue number | 4 |
| Publication status | Published - 2001 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Applied Microbiology and Biotechnology
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Recovery of cholesterol from the β-cyclodextrin-cholesterol complex using immobilized cyclomaltodextrinase of alkalophilic Bacillus sp. KJ 133. / Kwon, H. J.; Jung, H. J.; Kwak, H. S.
In: Journal of Microbiology and Biotechnology, Vol. 11, No. 4, 01.01.2001, p. 712-715.Research output: Contribution to journal › Article
TY - JOUR
T1 - Recovery of cholesterol from the β-cyclodextrin-cholesterol complex using immobilized cyclomaltodextrinase of alkalophilic Bacillus sp. KJ 133
AU - Kwon, H. J.
AU - Jung, H. J.
AU - Kwak, H. S.
PY - 2001/1/1
Y1 - 2001/1/1
N2 - A new combined method including the enzymatic hydrolysis of β-cyclodextrin (β-CD) and solvent extraction of cholesterol from the hydrolyzed mixture was developed to recover cholesterol from a β-CD-cholesterol complex prepared from dairy products, such as cream, milk, and cheese. Cyclomaltodextrinase (cyclomaltodextrin dextrin hydrolase, EC 3.2.1.54, CDase) prepared from alkalophilic Bacillus sp. KJ 133 hydrolyzed the β-CD of the β-CD-cholesterol complex, and then, free cholesterol was efficiently extracted from the hydrolyzed mixture by a nonpolar solvent such as ethyl acetate. To increase the stability of free CDase, immobilized CDase was developed using sodium alginate as a carrier. The immobilized CDase showed a high recovery yield of cholesterol in a time-dependent manner compared to the free CDase. A gas chromatography analysis showed that more than 70% of cholesterol was recovered from the β-CD-cholesterol complex of cream by the immobilized CDase, whereas only 3% and 29% of cholesterol were recovered when the solvent extraction and free CDase treatment were used, respectively. The cholesterol recovered can be used as a raw material for steroid synthesis. Furthermore, this method can be an efficient way to recover cholesterol or other organic compounds that are bound in a β-CD-cholesterol or -organic compound complex.
AB - A new combined method including the enzymatic hydrolysis of β-cyclodextrin (β-CD) and solvent extraction of cholesterol from the hydrolyzed mixture was developed to recover cholesterol from a β-CD-cholesterol complex prepared from dairy products, such as cream, milk, and cheese. Cyclomaltodextrinase (cyclomaltodextrin dextrin hydrolase, EC 3.2.1.54, CDase) prepared from alkalophilic Bacillus sp. KJ 133 hydrolyzed the β-CD of the β-CD-cholesterol complex, and then, free cholesterol was efficiently extracted from the hydrolyzed mixture by a nonpolar solvent such as ethyl acetate. To increase the stability of free CDase, immobilized CDase was developed using sodium alginate as a carrier. The immobilized CDase showed a high recovery yield of cholesterol in a time-dependent manner compared to the free CDase. A gas chromatography analysis showed that more than 70% of cholesterol was recovered from the β-CD-cholesterol complex of cream by the immobilized CDase, whereas only 3% and 29% of cholesterol were recovered when the solvent extraction and free CDase treatment were used, respectively. The cholesterol recovered can be used as a raw material for steroid synthesis. Furthermore, this method can be an efficient way to recover cholesterol or other organic compounds that are bound in a β-CD-cholesterol or -organic compound complex.
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M3 - Article
VL - 11
SP - 712
EP - 715
JO - Journal of Microbiology and Biotechnology
JF - Journal of Microbiology and Biotechnology
SN - 1017-7825
IS - 4
ER -