Recovery of cholesterol from the β-cyclodextrin-cholesterol complex using immobilized cyclomaltodextrinase of alkalophilic Bacillus sp. KJ 133

H. J. Kwon, H. J. Jung, H. S. Kwak

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Abstract

A new combined method including the enzymatic hydrolysis of β-cyclodextrin (β-CD) and solvent extraction of cholesterol from the hydrolyzed mixture was developed to recover cholesterol from a β-CD-cholesterol complex prepared from dairy products, such as cream, milk, and cheese. Cyclomaltodextrinase (cyclomaltodextrin dextrin hydrolase, EC 3.2.1.54, CDase) prepared from alkalophilic Bacillus sp. KJ 133 hydrolyzed the β-CD of the β-CD-cholesterol complex, and then, free cholesterol was efficiently extracted from the hydrolyzed mixture by a nonpolar solvent such as ethyl acetate. To increase the stability of free CDase, immobilized CDase was developed using sodium alginate as a carrier. The immobilized CDase showed a high recovery yield of cholesterol in a time-dependent manner compared to the free CDase. A gas chromatography analysis showed that more than 70% of cholesterol was recovered from the β-CD-cholesterol complex of cream by the immobilized CDase, whereas only 3% and 29% of cholesterol were recovered when the solvent extraction and free CDase treatment were used, respectively. The cholesterol recovered can be used as a raw material for steroid synthesis. Furthermore, this method can be an efficient way to recover cholesterol or other organic compounds that are bound in a β-CD-cholesterol or -organic compound complex.

Original languageEnglish
Pages (from-to)712-715
Number of pages4
JournalJournal of Microbiology and Biotechnology
Volume11
Issue number4
Publication statusPublished - 2001 Jan 1

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cyclomaltodextrinase
Cyclodextrins
Bacillus
Cholesterol
Hydrolases

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

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title = "Recovery of cholesterol from the β-cyclodextrin-cholesterol complex using immobilized cyclomaltodextrinase of alkalophilic Bacillus sp. KJ 133",
abstract = "A new combined method including the enzymatic hydrolysis of β-cyclodextrin (β-CD) and solvent extraction of cholesterol from the hydrolyzed mixture was developed to recover cholesterol from a β-CD-cholesterol complex prepared from dairy products, such as cream, milk, and cheese. Cyclomaltodextrinase (cyclomaltodextrin dextrin hydrolase, EC 3.2.1.54, CDase) prepared from alkalophilic Bacillus sp. KJ 133 hydrolyzed the β-CD of the β-CD-cholesterol complex, and then, free cholesterol was efficiently extracted from the hydrolyzed mixture by a nonpolar solvent such as ethyl acetate. To increase the stability of free CDase, immobilized CDase was developed using sodium alginate as a carrier. The immobilized CDase showed a high recovery yield of cholesterol in a time-dependent manner compared to the free CDase. A gas chromatography analysis showed that more than 70{\%} of cholesterol was recovered from the β-CD-cholesterol complex of cream by the immobilized CDase, whereas only 3{\%} and 29{\%} of cholesterol were recovered when the solvent extraction and free CDase treatment were used, respectively. The cholesterol recovered can be used as a raw material for steroid synthesis. Furthermore, this method can be an efficient way to recover cholesterol or other organic compounds that are bound in a β-CD-cholesterol or -organic compound complex.",
author = "Kwon, {H. J.} and Jung, {H. J.} and Kwak, {H. S.}",
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T1 - Recovery of cholesterol from the β-cyclodextrin-cholesterol complex using immobilized cyclomaltodextrinase of alkalophilic Bacillus sp. KJ 133

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AU - Jung, H. J.

AU - Kwak, H. S.

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N2 - A new combined method including the enzymatic hydrolysis of β-cyclodextrin (β-CD) and solvent extraction of cholesterol from the hydrolyzed mixture was developed to recover cholesterol from a β-CD-cholesterol complex prepared from dairy products, such as cream, milk, and cheese. Cyclomaltodextrinase (cyclomaltodextrin dextrin hydrolase, EC 3.2.1.54, CDase) prepared from alkalophilic Bacillus sp. KJ 133 hydrolyzed the β-CD of the β-CD-cholesterol complex, and then, free cholesterol was efficiently extracted from the hydrolyzed mixture by a nonpolar solvent such as ethyl acetate. To increase the stability of free CDase, immobilized CDase was developed using sodium alginate as a carrier. The immobilized CDase showed a high recovery yield of cholesterol in a time-dependent manner compared to the free CDase. A gas chromatography analysis showed that more than 70% of cholesterol was recovered from the β-CD-cholesterol complex of cream by the immobilized CDase, whereas only 3% and 29% of cholesterol were recovered when the solvent extraction and free CDase treatment were used, respectively. The cholesterol recovered can be used as a raw material for steroid synthesis. Furthermore, this method can be an efficient way to recover cholesterol or other organic compounds that are bound in a β-CD-cholesterol or -organic compound complex.

AB - A new combined method including the enzymatic hydrolysis of β-cyclodextrin (β-CD) and solvent extraction of cholesterol from the hydrolyzed mixture was developed to recover cholesterol from a β-CD-cholesterol complex prepared from dairy products, such as cream, milk, and cheese. Cyclomaltodextrinase (cyclomaltodextrin dextrin hydrolase, EC 3.2.1.54, CDase) prepared from alkalophilic Bacillus sp. KJ 133 hydrolyzed the β-CD of the β-CD-cholesterol complex, and then, free cholesterol was efficiently extracted from the hydrolyzed mixture by a nonpolar solvent such as ethyl acetate. To increase the stability of free CDase, immobilized CDase was developed using sodium alginate as a carrier. The immobilized CDase showed a high recovery yield of cholesterol in a time-dependent manner compared to the free CDase. A gas chromatography analysis showed that more than 70% of cholesterol was recovered from the β-CD-cholesterol complex of cream by the immobilized CDase, whereas only 3% and 29% of cholesterol were recovered when the solvent extraction and free CDase treatment were used, respectively. The cholesterol recovered can be used as a raw material for steroid synthesis. Furthermore, this method can be an efficient way to recover cholesterol or other organic compounds that are bound in a β-CD-cholesterol or -organic compound complex.

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