Redox cycling-based immunoassay for detection of carcinogenic embryonic antigen

Ga Yeon Lee, Jun Hee Park, Young Wook Chang, Sungbo Cho, Min Jung Kang, Jae Chul Pyun

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Redox cycling based on an interdigitated electrode (IDE) was used as a highly sensitive immunoassay for carcinogenic embryonic antigen (CEA) through the quantification of 3,3′,5,5′-tetramethylbenzidine (TMB). For the redox cycling process, one pair of interdigitated finger electrodes was used as the first working electrode (generator) for cyclic voltammetry of TMB, and another pair of interdigitated finger electrodes was used as the second working electrode (collector) for sequential application of potentials for reduction and oxidation of TMB. The reduction (and oxidation) products of TMB at the collector were supplied to the generator, and following sequential oxidization (and reduction) at the generator, again supplied to the collector. Such redox recycling processes between the generator and collector allowed signal amplification. In this work, the influences of the following factors on the redox cycling of TMB were analyzed: (1) the redox potential at the collector, (2) the gap between the interdigitated finger electrodes, and (3) the scan rate of the generator. The redox potential and electrode gap influences were simulated with COMSOL software and compared with empirical results. At the optimum redox potentials and electrode gap, redox cycling was estimated to be five-fold more sensitive for the quantification of TMB than conventional cyclic voltammetry using one pair of interdigitated finger electrodes as the working electrode. Finally, redox cycling was applied to a commercial immunoassay for CEA, and the sensitivity of redox cycling was three-fold higher than that of conventional cyclic voltammetry using a single set of interdigitated finger electrodes as the working electrode.

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalAnalytica Chimica Acta
Volume971
DOIs
Publication statusPublished - 2017 Jun 8

Fingerprint

immunoassay
antigen
Immunoassay
Oxidation-Reduction
Electrodes
electrode
Antigens
Fingers
redox potential
Cyclic voltammetry
detection
fold
oxidation
Oxidation
Recycling
Amplification
amplification
Software
recycling

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

Cite this

Lee, Ga Yeon ; Park, Jun Hee ; Chang, Young Wook ; Cho, Sungbo ; Kang, Min Jung ; Pyun, Jae Chul. / Redox cycling-based immunoassay for detection of carcinogenic embryonic antigen. In: Analytica Chimica Acta. 2017 ; Vol. 971. pp. 33-39.
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Redox cycling-based immunoassay for detection of carcinogenic embryonic antigen. / Lee, Ga Yeon; Park, Jun Hee; Chang, Young Wook; Cho, Sungbo; Kang, Min Jung; Pyun, Jae Chul.

In: Analytica Chimica Acta, Vol. 971, 08.06.2017, p. 33-39.

Research output: Contribution to journalArticle

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