Reduced expression of 1-cys peroxiredoxin in oxidative stress-induced cataracts

Jhang Ho Pak, Tae Im Kim, Myoung Joon Kim, Jae Yong Kim, Hyun Jeung Choi, Seon Ah Kim, Hungwon Tchah

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

1-cys peroxiredoxin (1-cysPrx), a member of the peroxiredoxin family with a single conserved cysteine residue, reduces a broad spectrum of hydroperoxides. This study was undertaken to examine changes in 1-cysPrx expression in human cataract samples, human lens epithelial (HLE B3) cell line, and rat organ-cultured lenses in response to oxidative insult induced by H 2O2 or transforming growth factor-beta1 (TGF-β1). Expression of 1-cysPrx mRNA and protein in HLE B3 cells increased in response to 2-8 ng ml-1 TGF-β1 and 50-75 μm H2O2 and then decreased below the control level at high doses (10 ng ml-1 TGF-β1 and 100-150 μm H2O2), as determined by Northern blot and immunoblot analysis. This reduction coincided with the decrease of cell viability. Immunoreactive 1-cysPrx protein was measured in capsulorrhexis specimens obtained from patients with anterior subcapsular cataract (ASC), nuclear sclerosis (NS), cortical spokes (CS), posterior subcapsular cataract (PSC), or white mature cataract (WC) at the time of cataract surgery. Significant reduction of 1-cysPrx protein was observed in ASC, PSC, and WC samples, but there was no statistical difference in CS and NS samples relative to normal control. Also, rat lens explants were cultured with 10 ng ml-1 TGF-β1 for ∼5 days or 500 μm H 2O2 for ∼2 days. Subsequently, expression of 1-cysPrx mRNA and protein in the lens capsules was evaluated. Rat lens explants treated with TGF-β1 or H2O2 developed a cataract similar to human ASC or WC, respectively, which resulted in a markedly decreased expression of 1-cysPrx mRNA and protein. Collectively, these findings show that expression patterns of 1-cysPrx gene in the lens are changed in response to oxidative stress, a major factor in the etiology of cataract.

Original languageEnglish
Pages (from-to)899-906
Number of pages8
JournalExperimental Eye Research
Volume82
Issue number5
DOIs
Publication statusPublished - 2006 May 1

Fingerprint

Peroxiredoxins
Cataract
Oxidative Stress
Transforming Growth Factor beta1
Lenses
Sclerosis
Messenger RNA
Proteins
Capsulorhexis
Crystallins
Northern Blotting
Hydrogen Peroxide
Capsules
Cysteine
Cell Survival

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Pak, Jhang Ho ; Kim, Tae Im ; Joon Kim, Myoung ; Yong Kim, Jae ; Choi, Hyun Jeung ; Kim, Seon Ah ; Tchah, Hungwon. / Reduced expression of 1-cys peroxiredoxin in oxidative stress-induced cataracts. In: Experimental Eye Research. 2006 ; Vol. 82, No. 5. pp. 899-906.
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title = "Reduced expression of 1-cys peroxiredoxin in oxidative stress-induced cataracts",
abstract = "1-cys peroxiredoxin (1-cysPrx), a member of the peroxiredoxin family with a single conserved cysteine residue, reduces a broad spectrum of hydroperoxides. This study was undertaken to examine changes in 1-cysPrx expression in human cataract samples, human lens epithelial (HLE B3) cell line, and rat organ-cultured lenses in response to oxidative insult induced by H 2O2 or transforming growth factor-beta1 (TGF-β1). Expression of 1-cysPrx mRNA and protein in HLE B3 cells increased in response to 2-8 ng ml-1 TGF-β1 and 50-75 μm H2O2 and then decreased below the control level at high doses (10 ng ml-1 TGF-β1 and 100-150 μm H2O2), as determined by Northern blot and immunoblot analysis. This reduction coincided with the decrease of cell viability. Immunoreactive 1-cysPrx protein was measured in capsulorrhexis specimens obtained from patients with anterior subcapsular cataract (ASC), nuclear sclerosis (NS), cortical spokes (CS), posterior subcapsular cataract (PSC), or white mature cataract (WC) at the time of cataract surgery. Significant reduction of 1-cysPrx protein was observed in ASC, PSC, and WC samples, but there was no statistical difference in CS and NS samples relative to normal control. Also, rat lens explants were cultured with 10 ng ml-1 TGF-β1 for ∼5 days or 500 μm H 2O2 for ∼2 days. Subsequently, expression of 1-cysPrx mRNA and protein in the lens capsules was evaluated. Rat lens explants treated with TGF-β1 or H2O2 developed a cataract similar to human ASC or WC, respectively, which resulted in a markedly decreased expression of 1-cysPrx mRNA and protein. Collectively, these findings show that expression patterns of 1-cysPrx gene in the lens are changed in response to oxidative stress, a major factor in the etiology of cataract.",
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Pak, JH, Kim, TI, Joon Kim, M, Yong Kim, J, Choi, HJ, Kim, SA & Tchah, H 2006, 'Reduced expression of 1-cys peroxiredoxin in oxidative stress-induced cataracts', Experimental Eye Research, vol. 82, no. 5, pp. 899-906. https://doi.org/10.1016/j.exer.2005.10.017

Reduced expression of 1-cys peroxiredoxin in oxidative stress-induced cataracts. / Pak, Jhang Ho; Kim, Tae Im; Joon Kim, Myoung; Yong Kim, Jae; Choi, Hyun Jeung; Kim, Seon Ah; Tchah, Hungwon.

In: Experimental Eye Research, Vol. 82, No. 5, 01.05.2006, p. 899-906.

Research output: Contribution to journalArticle

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AU - Kim, Seon Ah

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