Although the role of autophagy in tumorigenesis remains controversial, recent reports support the notion that inhibition of autophagy promotes tumor formation. Damage-regulated autophagy regulator (DRAM) has been identified as an effector molecule that is critical for p53-mediated apoptosis, and we investigated whether there might be other DRAM-like molecules linking autophagy and apoptosis. In this study, we cloned a novel DRAM-homologous protein, DRAM2, and showed that the expression of DRAM2 is down-regulated in ovarian tumors. DRAM2 is mainly localized in the lysosome, and co-localizes with DRAM. While expression of DRAM or DRAM2 individually did not induce cell death, co-expression of DRAM2 with DRAM significantly induced cell death, while the silencing of endogenous DRAM2 attenuated cell death, suggesting that DRAM2 is involved in cell death. Thus, we propose that reduced expression of DRAM2 may contribute to enhanced cell survival in tumor cells.
|Number of pages||5|
|Journal||Biochemical and Biophysical Research Communications|
|Publication status||Published - 2009 Dec 25|
Bibliographical noteFunding Information:
We would particularly like to thank Kevin M. Ryan for providing the DRAM expression plasmid. This study was supported by a Science Research Center (Molecular Therapy Research Center) grant from the Korea Science and Engineering Foundation , and by a Korea Basic Science Institute grant (K-MeP). The nucleotide and deduced amino acid sequences of DRAM2 were submitted and registered in GenBank (Accession No. EF710624.1 ).
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology