Reduced imipenem susceptibility in Klebsiella pneumoniae clinical isolates with plasmid-mediated CMY-2 and DHA-1 β-lactamases co-mediated by porin loss

Kyungwon Lee, DongEun Yong, Yeong Seon Choi, Jong Hwa Yum, June Myung Kim, Neil Woodford, David M. Livermore, Yunsop Chong

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Abstract

We investigated the resistance mechanisms and clonality among 42 imipenem-non-susceptible Klebsiella pneumoniae isolated at a tertiary care hospital in Korea. Two isolates had blaVIM-2 alleles, whereas blaCMY-2- and blaDHA-1-like alleles were detected in 24 and 16 isolates, respectively, with these enzymes confirmed by sequencing for representative isolates. Transfer of blaCMY-2 and blaDHA-1 was achieved by conjugation. Addition of 300 mg/L 3-aminophenylboronic acid (APB) reduced the minimum inhibitory concentration for 90% of the organisms (MIC90) of imipenem and meropenem eight- and four-fold, respectively, for the blaCMY-2- and blaDHA-1-positive isolates, confirming the role of these enzymes in resistance. SDS-PAGE of outer membrane proteins for representative isolates showed lack or greatly diminished expression of OmpK35 and OmpK36 porins. Pulsed-field gel electrophoresis of XbaI-restricted genomic DNA revealed two closely related clusters among 23 blaCMY-2-positive isolates, whereas those with blaDHA-1 were more heterogeneous. In conclusion, reduced imipenem susceptibility among K. pneumoniae at this Korean hospital was largely co-mediated by production of plasmid-mediated AmpC β-lactamases along with lack or greatly diminished expression of OmpK35 and OmpK36 porins.

Original languageEnglish
Pages (from-to)201-206
Number of pages6
JournalInternational Journal of Antimicrobial Agents
Volume29
Issue number2
DOIs
Publication statusPublished - 2007 Feb 1

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Porins
Imipenem
Klebsiella pneumoniae
Plasmids
meropenem
Alleles
Pulsed Field Gel Electrophoresis
Microbial Sensitivity Tests
Enzymes
Tertiary Healthcare
Korea
Tertiary Care Centers
Polyacrylamide Gel Electrophoresis
Membrane Proteins
DNA

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

Cite this

Lee, Kyungwon ; Yong, DongEun ; Choi, Yeong Seon ; Yum, Jong Hwa ; Kim, June Myung ; Woodford, Neil ; Livermore, David M. ; Chong, Yunsop. / Reduced imipenem susceptibility in Klebsiella pneumoniae clinical isolates with plasmid-mediated CMY-2 and DHA-1 β-lactamases co-mediated by porin loss. In: International Journal of Antimicrobial Agents. 2007 ; Vol. 29, No. 2. pp. 201-206.
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abstract = "We investigated the resistance mechanisms and clonality among 42 imipenem-non-susceptible Klebsiella pneumoniae isolated at a tertiary care hospital in Korea. Two isolates had blaVIM-2 alleles, whereas blaCMY-2- and blaDHA-1-like alleles were detected in 24 and 16 isolates, respectively, with these enzymes confirmed by sequencing for representative isolates. Transfer of blaCMY-2 and blaDHA-1 was achieved by conjugation. Addition of 300 mg/L 3-aminophenylboronic acid (APB) reduced the minimum inhibitory concentration for 90{\%} of the organisms (MIC90) of imipenem and meropenem eight- and four-fold, respectively, for the blaCMY-2- and blaDHA-1-positive isolates, confirming the role of these enzymes in resistance. SDS-PAGE of outer membrane proteins for representative isolates showed lack or greatly diminished expression of OmpK35 and OmpK36 porins. Pulsed-field gel electrophoresis of XbaI-restricted genomic DNA revealed two closely related clusters among 23 blaCMY-2-positive isolates, whereas those with blaDHA-1 were more heterogeneous. In conclusion, reduced imipenem susceptibility among K. pneumoniae at this Korean hospital was largely co-mediated by production of plasmid-mediated AmpC β-lactamases along with lack or greatly diminished expression of OmpK35 and OmpK36 porins.",
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Reduced imipenem susceptibility in Klebsiella pneumoniae clinical isolates with plasmid-mediated CMY-2 and DHA-1 β-lactamases co-mediated by porin loss. / Lee, Kyungwon; Yong, DongEun; Choi, Yeong Seon; Yum, Jong Hwa; Kim, June Myung; Woodford, Neil; Livermore, David M.; Chong, Yunsop.

In: International Journal of Antimicrobial Agents, Vol. 29, No. 2, 01.02.2007, p. 201-206.

Research output: Contribution to journalArticle

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AU - Choi, Yeong Seon

AU - Yum, Jong Hwa

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AU - Woodford, Neil

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AU - Chong, Yunsop

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AB - We investigated the resistance mechanisms and clonality among 42 imipenem-non-susceptible Klebsiella pneumoniae isolated at a tertiary care hospital in Korea. Two isolates had blaVIM-2 alleles, whereas blaCMY-2- and blaDHA-1-like alleles were detected in 24 and 16 isolates, respectively, with these enzymes confirmed by sequencing for representative isolates. Transfer of blaCMY-2 and blaDHA-1 was achieved by conjugation. Addition of 300 mg/L 3-aminophenylboronic acid (APB) reduced the minimum inhibitory concentration for 90% of the organisms (MIC90) of imipenem and meropenem eight- and four-fold, respectively, for the blaCMY-2- and blaDHA-1-positive isolates, confirming the role of these enzymes in resistance. SDS-PAGE of outer membrane proteins for representative isolates showed lack or greatly diminished expression of OmpK35 and OmpK36 porins. Pulsed-field gel electrophoresis of XbaI-restricted genomic DNA revealed two closely related clusters among 23 blaCMY-2-positive isolates, whereas those with blaDHA-1 were more heterogeneous. In conclusion, reduced imipenem susceptibility among K. pneumoniae at this Korean hospital was largely co-mediated by production of plasmid-mediated AmpC β-lactamases along with lack or greatly diminished expression of OmpK35 and OmpK36 porins.

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