Reduction of adipose tissue formation by the controlled release of BMP-2 using a hydroxyapatite-coated collagen carrier system for sinus-augmentation/extraction-socket grafting

Jung Seok Lee, Tae Wan Kim, Soyon Park, Byung Soo Kim, Gun Il Im, Kyoo Sung Cho, Chang Sung Kim

Research output: Contribution to journalArticle

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Abstract

The effects of hydroxyapatite (HA)-coating onto collagen carriers for application of recombinant human bone morphogenetic protein 2 (rhBMP-2) on cell differentiation in vitro, and on in vivo healing patterns after sinus-augmentation and alveolar socket-grafting were evaluated. In vitro induction of osteogenic/adipogenic differentiation was compared between the culture media with rhBMP-2 solution and with the released rhBMP-2 from the control collagen and from the HA-coated collagen. Demineralized bovine bone and collagen/HA-coated collagen were grafted with/without rhBMP-2 in sinus-augmentation and tooth-extraction-socket models. Adipogenic induction by rhBMP-2 released from HA-coated collagen was significantly reduced compared to collagen. In the sinus-augmentation model, sites that received rhBMP-2 exhibited large amounts of vascular tissue formation at two weeks and increased adipose tissue formation at eight weeks; this could be significantly reduced by using HA-coated collagen as a carrier for rhBMP-2. In extraction-socket grafting, dimensional reduction of alveolar ridge was significantly decreased at sites received rhBMP-2 compared to control sites, but adipose tissue was increased within the regenerated socket area. In conclusion, HA-coated collagen carrier for Escherichia coli-derived rhBMP-2 (ErhBMP-2) may reduce in vitro induction of adipogenic differentiation and in vivo adipose bone marrow tissue formation in bone tissue engineering by ErhBMP-2.

Original languageEnglish
Pages (from-to)7634-7649
Number of pages16
JournalMaterials
Volume8
Issue number11
DOIs
Publication statusPublished - 2015 Jan 1

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Durapatite
Hydroxyapatite
Collagen
Bone
Tissue
Proteins
recombinant human bone morphogenetic protein-2
Tissue engineering
Escherichia coli
Culture Media
Coatings

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

Lee, Jung Seok ; Kim, Tae Wan ; Park, Soyon ; Kim, Byung Soo ; Im, Gun Il ; Cho, Kyoo Sung ; Kim, Chang Sung. / Reduction of adipose tissue formation by the controlled release of BMP-2 using a hydroxyapatite-coated collagen carrier system for sinus-augmentation/extraction-socket grafting. In: Materials. 2015 ; Vol. 8, No. 11. pp. 7634-7649.
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abstract = "The effects of hydroxyapatite (HA)-coating onto collagen carriers for application of recombinant human bone morphogenetic protein 2 (rhBMP-2) on cell differentiation in vitro, and on in vivo healing patterns after sinus-augmentation and alveolar socket-grafting were evaluated. In vitro induction of osteogenic/adipogenic differentiation was compared between the culture media with rhBMP-2 solution and with the released rhBMP-2 from the control collagen and from the HA-coated collagen. Demineralized bovine bone and collagen/HA-coated collagen were grafted with/without rhBMP-2 in sinus-augmentation and tooth-extraction-socket models. Adipogenic induction by rhBMP-2 released from HA-coated collagen was significantly reduced compared to collagen. In the sinus-augmentation model, sites that received rhBMP-2 exhibited large amounts of vascular tissue formation at two weeks and increased adipose tissue formation at eight weeks; this could be significantly reduced by using HA-coated collagen as a carrier for rhBMP-2. In extraction-socket grafting, dimensional reduction of alveolar ridge was significantly decreased at sites received rhBMP-2 compared to control sites, but adipose tissue was increased within the regenerated socket area. In conclusion, HA-coated collagen carrier for Escherichia coli-derived rhBMP-2 (ErhBMP-2) may reduce in vitro induction of adipogenic differentiation and in vivo adipose bone marrow tissue formation in bone tissue engineering by ErhBMP-2.",
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Reduction of adipose tissue formation by the controlled release of BMP-2 using a hydroxyapatite-coated collagen carrier system for sinus-augmentation/extraction-socket grafting. / Lee, Jung Seok; Kim, Tae Wan; Park, Soyon; Kim, Byung Soo; Im, Gun Il; Cho, Kyoo Sung; Kim, Chang Sung.

In: Materials, Vol. 8, No. 11, 01.01.2015, p. 7634-7649.

Research output: Contribution to journalArticle

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