Regeneration Approach to Enhance the Antimicrobial and Antioxidant Activities of Chitosan for Biomedical Applications

Pradeep Kumar Panda, Kambiz Sadeghi, Kitae Park, Jongchul Seo

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1 Citation (Scopus)


Owing to its biodegradability, non-toxicity, and biocompatibility, chitosan (Cs) is a ubiquitous biopolymer. However, applications of Cs are limited owing to the existence of strong inter- and intra-molecular hydrogen bonds within its network. To address this issue, we regenerated medium-molecular-weight Cs to enhance the physico-chemical and functional properties using a cationic approach. Accordingly, alkaline modification was employed to introduce an additional positive charge to the amine functional groups of Cs and moderately disintegrate the inter- and intra-hydrogen bonds. The chemical structure of Cs and regenerated chitosan (RCs) was confirmed through Fourier transform infrared and 1H-NMR spectroscopy. RCs showed higher zeta potential value compared to Cs. Additionally, using X-ray diffraction, RCs exhibited low crystallinity, which can be attributed to the repulsive force caused by the positive surface charge and the destruction of hydrogen bonds. The RCs exhibited stronger antioxidant activity than Cs. Furthermore, the minimum inhibition concentrations (MICs) of RCs against Escherichia coli and Staphylococcus aureus were reduced by almost four times compared with those of Cs. The superior functional properties of RCs can be attributed to the formation of a polycationic structure after alkaline modification. Thus, RCs can be introduced as potent agents for various biomedical purposes.

Original languageEnglish
Article number132
Issue number1
Publication statusPublished - 2023 Jan

Bibliographical note

Funding Information:
This study was supported by the Technology Development Program funded the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (grant number 2020R1A2B5B010017970). This work also supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through (High value-added Food Technology Development Program), funded by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (grant number 321048041HD030).

Publisher Copyright:
© 2022 by the authors.

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Polymers and Plastics


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