Region-specific changes in the immunoreactivity of SIRT1 expression in the central nervous system of SOD1 G93A transgenic mice as an in vivo model of amyotrophic lateral sclerosis

Jae Chul Lee, Ji Hee Shin, Byeongwoo Park, Gwon Sik Kim, Jin Cheon Kim, Kyung Sun Kang, Choong Ik Cha

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

SIRT1, which is a mammalian homolog of yeast nicotinamide adenine dinucleotide-dependent deacetylase silent information regulator 2 (SIR2), is the best-characterized SIRT family member. SIRT1 regulates longevity in several model organisms and is involved in several processes in mammalian cells, including cell survival, differentiation, and metabolism. In the present study, we used SOD1 G93A mutant transgenic mice as an in vivo model of amyotrophic lateral sclerosis (ALS) and performed immunohistochemical studies, RT-PCR and western blotting analysis in order to investigate the changes of SIRT1 immunoreactivity in the central nervous system of these mice. An increased expression of SIRT1 was obvious in the cerebral cortex, hippocampal formation, thalamus and spinal cord of symptomatic SOD1 G93A transgenic mice. In the cerebral cortex, SIRT1 immunoreactivity was significantly increased in pyramidal cells of SOD1 G93A transgenic mice. In the hippocampal formation of these mice, SIRT1 immunoreactivity was increased in the pyramidal cells of the CA1-3 areas and in the granule cells of the dentate gyrus. In addition, SIRT1 immunoreactivity was increased in the spinal cord and thalamus of symptomatic SOD1 G93A transgenic mice. This study, which showed increased SIRT1 in different brain regions of SOD1 G93A transgenic mice, may provide clues to the understanding of selective neuronal loss in ALS. These findings suggest a role for SIRT1 in the motor functions in ALS but the mechanisms and functional implications of increased SIRT1 require elucidation.

Original languageEnglish
Pages (from-to)20-28
Number of pages9
JournalBrain Research
Volume1433
DOIs
Publication statusPublished - 2012 Jan 18

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Amyotrophic Lateral Sclerosis
Transgenic Mice
Central Nervous System
Thalamus
Cerebral Cortex
Hippocampus
Spinal Cord
Neurologic Mutant Mice
Hippocampal CA1 Region
Pyramidal Cells
Dentate Gyrus
NAD
Cell Differentiation
Cell Survival
Yeasts
Western Blotting
Polymerase Chain Reaction
Brain

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

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title = "Region-specific changes in the immunoreactivity of SIRT1 expression in the central nervous system of SOD1 G93A transgenic mice as an in vivo model of amyotrophic lateral sclerosis",
abstract = "SIRT1, which is a mammalian homolog of yeast nicotinamide adenine dinucleotide-dependent deacetylase silent information regulator 2 (SIR2), is the best-characterized SIRT family member. SIRT1 regulates longevity in several model organisms and is involved in several processes in mammalian cells, including cell survival, differentiation, and metabolism. In the present study, we used SOD1 G93A mutant transgenic mice as an in vivo model of amyotrophic lateral sclerosis (ALS) and performed immunohistochemical studies, RT-PCR and western blotting analysis in order to investigate the changes of SIRT1 immunoreactivity in the central nervous system of these mice. An increased expression of SIRT1 was obvious in the cerebral cortex, hippocampal formation, thalamus and spinal cord of symptomatic SOD1 G93A transgenic mice. In the cerebral cortex, SIRT1 immunoreactivity was significantly increased in pyramidal cells of SOD1 G93A transgenic mice. In the hippocampal formation of these mice, SIRT1 immunoreactivity was increased in the pyramidal cells of the CA1-3 areas and in the granule cells of the dentate gyrus. In addition, SIRT1 immunoreactivity was increased in the spinal cord and thalamus of symptomatic SOD1 G93A transgenic mice. This study, which showed increased SIRT1 in different brain regions of SOD1 G93A transgenic mice, may provide clues to the understanding of selective neuronal loss in ALS. These findings suggest a role for SIRT1 in the motor functions in ALS but the mechanisms and functional implications of increased SIRT1 require elucidation.",
author = "Lee, {Jae Chul} and Shin, {Ji Hee} and Byeongwoo Park and Kim, {Gwon Sik} and Kim, {Jin Cheon} and Kang, {Kyung Sun} and Cha, {Choong Ik}",
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Region-specific changes in the immunoreactivity of SIRT1 expression in the central nervous system of SOD1 G93A transgenic mice as an in vivo model of amyotrophic lateral sclerosis . / Lee, Jae Chul; Shin, Ji Hee; Park, Byeongwoo; Kim, Gwon Sik; Kim, Jin Cheon; Kang, Kyung Sun; Cha, Choong Ik.

In: Brain Research, Vol. 1433, 18.01.2012, p. 20-28.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Region-specific changes in the immunoreactivity of SIRT1 expression in the central nervous system of SOD1 G93A transgenic mice as an in vivo model of amyotrophic lateral sclerosis

AU - Lee, Jae Chul

AU - Shin, Ji Hee

AU - Park, Byeongwoo

AU - Kim, Gwon Sik

AU - Kim, Jin Cheon

AU - Kang, Kyung Sun

AU - Cha, Choong Ik

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