Abstract
SIRT1, which is a mammalian homolog of yeast nicotinamide adenine dinucleotide-dependent deacetylase silent information regulator 2 (SIR2), is the best-characterized SIRT family member. SIRT1 regulates longevity in several model organisms and is involved in several processes in mammalian cells, including cell survival, differentiation, and metabolism. In the present study, we used SOD1 G93A mutant transgenic mice as an in vivo model of amyotrophic lateral sclerosis (ALS) and performed immunohistochemical studies, RT-PCR and western blotting analysis in order to investigate the changes of SIRT1 immunoreactivity in the central nervous system of these mice. An increased expression of SIRT1 was obvious in the cerebral cortex, hippocampal formation, thalamus and spinal cord of symptomatic SOD1 G93A transgenic mice. In the cerebral cortex, SIRT1 immunoreactivity was significantly increased in pyramidal cells of SOD1 G93A transgenic mice. In the hippocampal formation of these mice, SIRT1 immunoreactivity was increased in the pyramidal cells of the CA1-3 areas and in the granule cells of the dentate gyrus. In addition, SIRT1 immunoreactivity was increased in the spinal cord and thalamus of symptomatic SOD1 G93A transgenic mice. This study, which showed increased SIRT1 in different brain regions of SOD1 G93A transgenic mice, may provide clues to the understanding of selective neuronal loss in ALS. These findings suggest a role for SIRT1 in the motor functions in ALS but the mechanisms and functional implications of increased SIRT1 require elucidation.
| Original language | English |
|---|---|
| Pages (from-to) | 20-28 |
| Number of pages | 9 |
| Journal | Brain Research |
| Volume | 1433 |
| DOIs | |
| Publication status | Published - 2012 Jan 18 |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
- Molecular Biology
- Clinical Neurology
- Developmental Biology
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Region-specific changes in the immunoreactivity of SIRT1 expression in the central nervous system of SOD1 G93A transgenic mice as an in vivo model of amyotrophic lateral sclerosis . / Lee, Jae Chul; Shin, Ji Hee; Park, Byeongwoo; Kim, Gwon Sik; Kim, Jin Cheon; Kang, Kyung Sun; Cha, Choong Ik.
In: Brain Research, Vol. 1433, 18.01.2012, p. 20-28.Research output: Contribution to journal › Article
TY - JOUR
T1 - Region-specific changes in the immunoreactivity of SIRT1 expression in the central nervous system of SOD1 G93A transgenic mice as an in vivo model of amyotrophic lateral sclerosis
AU - Lee, Jae Chul
AU - Shin, Ji Hee
AU - Park, Byeongwoo
AU - Kim, Gwon Sik
AU - Kim, Jin Cheon
AU - Kang, Kyung Sun
AU - Cha, Choong Ik
PY - 2012/1/18
Y1 - 2012/1/18
N2 - SIRT1, which is a mammalian homolog of yeast nicotinamide adenine dinucleotide-dependent deacetylase silent information regulator 2 (SIR2), is the best-characterized SIRT family member. SIRT1 regulates longevity in several model organisms and is involved in several processes in mammalian cells, including cell survival, differentiation, and metabolism. In the present study, we used SOD1 G93A mutant transgenic mice as an in vivo model of amyotrophic lateral sclerosis (ALS) and performed immunohistochemical studies, RT-PCR and western blotting analysis in order to investigate the changes of SIRT1 immunoreactivity in the central nervous system of these mice. An increased expression of SIRT1 was obvious in the cerebral cortex, hippocampal formation, thalamus and spinal cord of symptomatic SOD1 G93A transgenic mice. In the cerebral cortex, SIRT1 immunoreactivity was significantly increased in pyramidal cells of SOD1 G93A transgenic mice. In the hippocampal formation of these mice, SIRT1 immunoreactivity was increased in the pyramidal cells of the CA1-3 areas and in the granule cells of the dentate gyrus. In addition, SIRT1 immunoreactivity was increased in the spinal cord and thalamus of symptomatic SOD1 G93A transgenic mice. This study, which showed increased SIRT1 in different brain regions of SOD1 G93A transgenic mice, may provide clues to the understanding of selective neuronal loss in ALS. These findings suggest a role for SIRT1 in the motor functions in ALS but the mechanisms and functional implications of increased SIRT1 require elucidation.
AB - SIRT1, which is a mammalian homolog of yeast nicotinamide adenine dinucleotide-dependent deacetylase silent information regulator 2 (SIR2), is the best-characterized SIRT family member. SIRT1 regulates longevity in several model organisms and is involved in several processes in mammalian cells, including cell survival, differentiation, and metabolism. In the present study, we used SOD1 G93A mutant transgenic mice as an in vivo model of amyotrophic lateral sclerosis (ALS) and performed immunohistochemical studies, RT-PCR and western blotting analysis in order to investigate the changes of SIRT1 immunoreactivity in the central nervous system of these mice. An increased expression of SIRT1 was obvious in the cerebral cortex, hippocampal formation, thalamus and spinal cord of symptomatic SOD1 G93A transgenic mice. In the cerebral cortex, SIRT1 immunoreactivity was significantly increased in pyramidal cells of SOD1 G93A transgenic mice. In the hippocampal formation of these mice, SIRT1 immunoreactivity was increased in the pyramidal cells of the CA1-3 areas and in the granule cells of the dentate gyrus. In addition, SIRT1 immunoreactivity was increased in the spinal cord and thalamus of symptomatic SOD1 G93A transgenic mice. This study, which showed increased SIRT1 in different brain regions of SOD1 G93A transgenic mice, may provide clues to the understanding of selective neuronal loss in ALS. These findings suggest a role for SIRT1 in the motor functions in ALS but the mechanisms and functional implications of increased SIRT1 require elucidation.
UR - http://www.scopus.com/inward/record.url?scp=84855200886&partnerID=8YFLogxK
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U2 - 10.1016/j.brainres.2011.11.019
DO - 10.1016/j.brainres.2011.11.019
M3 - Article
VL - 1433
SP - 20
EP - 28
JO - Brain Research
JF - Brain Research
SN - 0006-8993
ER -