Abstract
PTF (PSE-binding transcription factor) activates transcription of snRNA and related genes. We investigated its distribution in HeLa nuclei by immunofluorescence, and found it spread throughout the nucleoplasm in small foci. In some cells, PTF is also concentrated in one, or very few, discrete regions (diameter ~ 1.3 μm) that appear during G1 phase and disappear in S phase. Oct1, a transcription factor that interacts with PTF, is also enriched in these domains; RNA polymerase II, TBP and Sp1 are also present. Each domain typically contains 2 or 3 transcription 'factories' where Br-UTP is incorporated into nascent transcripts. Accordingly, we have christened this region the Oct1/PTF/transcription (OPT) domain. It colocalizes with some, but not all, PIKA domains. It is distinct from other nuclear domains, including coiled bodies, gemini bodies, PML bodies and the perinucleolar compartment. A small region on chromosome 6 (band 6p21) containing only ~ 30 Mbp DNA, and chromosomes 6 and 7, associate with the domain significantly more than other chromosomes. The domains may act like nucleoli to bring particular genes on specific chromosomes together to a region where the appropriate transcription and processing factors are concentrated, thereby facilitating the expression of those genes.
| Original language | English |
|---|---|
| Pages (from-to) | 1768-1778 |
| Number of pages | 11 |
| Journal | EMBO Journal |
| Volume | 17 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1998 Mar 16 |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Immunology and Microbiology(all)
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Regional and temporal specialization in the nucleus : A transcriptionally-active nuclear domain rich in PTF, Oct1 and PIKA antigens associates with specific chromosomes early in the cell cycle. / Pombo, Ana; Cuello, Paula; Schul, Wouter; Yoon, Jong Bok; Roeder, Robert G.; Cook, Peter R.; Murphy, Shona.
In: EMBO Journal, Vol. 17, No. 6, 16.03.1998, p. 1768-1778.Research output: Contribution to journal › Article
TY - JOUR
T1 - Regional and temporal specialization in the nucleus
T2 - A transcriptionally-active nuclear domain rich in PTF, Oct1 and PIKA antigens associates with specific chromosomes early in the cell cycle
AU - Pombo, Ana
AU - Cuello, Paula
AU - Schul, Wouter
AU - Yoon, Jong Bok
AU - Roeder, Robert G.
AU - Cook, Peter R.
AU - Murphy, Shona
PY - 1998/3/16
Y1 - 1998/3/16
N2 - PTF (PSE-binding transcription factor) activates transcription of snRNA and related genes. We investigated its distribution in HeLa nuclei by immunofluorescence, and found it spread throughout the nucleoplasm in small foci. In some cells, PTF is also concentrated in one, or very few, discrete regions (diameter ~ 1.3 μm) that appear during G1 phase and disappear in S phase. Oct1, a transcription factor that interacts with PTF, is also enriched in these domains; RNA polymerase II, TBP and Sp1 are also present. Each domain typically contains 2 or 3 transcription 'factories' where Br-UTP is incorporated into nascent transcripts. Accordingly, we have christened this region the Oct1/PTF/transcription (OPT) domain. It colocalizes with some, but not all, PIKA domains. It is distinct from other nuclear domains, including coiled bodies, gemini bodies, PML bodies and the perinucleolar compartment. A small region on chromosome 6 (band 6p21) containing only ~ 30 Mbp DNA, and chromosomes 6 and 7, associate with the domain significantly more than other chromosomes. The domains may act like nucleoli to bring particular genes on specific chromosomes together to a region where the appropriate transcription and processing factors are concentrated, thereby facilitating the expression of those genes.
AB - PTF (PSE-binding transcription factor) activates transcription of snRNA and related genes. We investigated its distribution in HeLa nuclei by immunofluorescence, and found it spread throughout the nucleoplasm in small foci. In some cells, PTF is also concentrated in one, or very few, discrete regions (diameter ~ 1.3 μm) that appear during G1 phase and disappear in S phase. Oct1, a transcription factor that interacts with PTF, is also enriched in these domains; RNA polymerase II, TBP and Sp1 are also present. Each domain typically contains 2 or 3 transcription 'factories' where Br-UTP is incorporated into nascent transcripts. Accordingly, we have christened this region the Oct1/PTF/transcription (OPT) domain. It colocalizes with some, but not all, PIKA domains. It is distinct from other nuclear domains, including coiled bodies, gemini bodies, PML bodies and the perinucleolar compartment. A small region on chromosome 6 (band 6p21) containing only ~ 30 Mbp DNA, and chromosomes 6 and 7, associate with the domain significantly more than other chromosomes. The domains may act like nucleoli to bring particular genes on specific chromosomes together to a region where the appropriate transcription and processing factors are concentrated, thereby facilitating the expression of those genes.
UR - http://www.scopus.com/inward/record.url?scp=0032536858&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032536858&partnerID=8YFLogxK
U2 - 10.1093/emboj/17.6.1768
DO - 10.1093/emboj/17.6.1768
M3 - Article
C2 - 9501098
AN - SCOPUS:0032536858
VL - 17
SP - 1768
EP - 1778
JO - EMBO Journal
JF - EMBO Journal
SN - 0261-4189
IS - 6
ER -