Regional and temporal specialization in the nucleus: A transcriptionally-active nuclear domain rich in PTF, Oct1 and PIKA antigens associates with specific chromosomes early in the cell cycle

Ana Pombo, Paula Cuello, Wouter Schul, Jong Bok Yoon, Robert G. Roeder, Peter R. Cook, Shona Murphy

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

PTF (PSE-binding transcription factor) activates transcription of snRNA and related genes. We investigated its distribution in HeLa nuclei by immunofluorescence, and found it spread throughout the nucleoplasm in small foci. In some cells, PTF is also concentrated in one, or very few, discrete regions (diameter ~ 1.3 μm) that appear during G1 phase and disappear in S phase. Oct1, a transcription factor that interacts with PTF, is also enriched in these domains; RNA polymerase II, TBP and Sp1 are also present. Each domain typically contains 2 or 3 transcription 'factories' where Br-UTP is incorporated into nascent transcripts. Accordingly, we have christened this region the Oct1/PTF/transcription (OPT) domain. It colocalizes with some, but not all, PIKA domains. It is distinct from other nuclear domains, including coiled bodies, gemini bodies, PML bodies and the perinucleolar compartment. A small region on chromosome 6 (band 6p21) containing only ~ 30 Mbp DNA, and chromosomes 6 and 7, associate with the domain significantly more than other chromosomes. The domains may act like nucleoli to bring particular genes on specific chromosomes together to a region where the appropriate transcription and processing factors are concentrated, thereby facilitating the expression of those genes.

Original languageEnglish
Pages (from-to)1768-1778
Number of pages11
JournalEMBO Journal
Volume17
Issue number6
DOIs
Publication statusPublished - 1998 Mar 16

Fingerprint

Chromosomes
Transcription
Cell Cycle
Transcription Factors
Cells
Antigens
Genes
Chromosomes, Human, Pair 6
Gemini of Coiled Bodies
Small Nuclear RNA
Uridine Triphosphate
RNA Polymerase II
Chromosomes, Human, Pair 7
G1 Phase
Industrial plants
S Phase
Fluorescent Antibody Technique
DNA
Gene Expression
Processing

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

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title = "Regional and temporal specialization in the nucleus: A transcriptionally-active nuclear domain rich in PTF, Oct1 and PIKA antigens associates with specific chromosomes early in the cell cycle",
abstract = "PTF (PSE-binding transcription factor) activates transcription of snRNA and related genes. We investigated its distribution in HeLa nuclei by immunofluorescence, and found it spread throughout the nucleoplasm in small foci. In some cells, PTF is also concentrated in one, or very few, discrete regions (diameter ~ 1.3 μm) that appear during G1 phase and disappear in S phase. Oct1, a transcription factor that interacts with PTF, is also enriched in these domains; RNA polymerase II, TBP and Sp1 are also present. Each domain typically contains 2 or 3 transcription 'factories' where Br-UTP is incorporated into nascent transcripts. Accordingly, we have christened this region the Oct1/PTF/transcription (OPT) domain. It colocalizes with some, but not all, PIKA domains. It is distinct from other nuclear domains, including coiled bodies, gemini bodies, PML bodies and the perinucleolar compartment. A small region on chromosome 6 (band 6p21) containing only ~ 30 Mbp DNA, and chromosomes 6 and 7, associate with the domain significantly more than other chromosomes. The domains may act like nucleoli to bring particular genes on specific chromosomes together to a region where the appropriate transcription and processing factors are concentrated, thereby facilitating the expression of those genes.",
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Regional and temporal specialization in the nucleus : A transcriptionally-active nuclear domain rich in PTF, Oct1 and PIKA antigens associates with specific chromosomes early in the cell cycle. / Pombo, Ana; Cuello, Paula; Schul, Wouter; Yoon, Jong Bok; Roeder, Robert G.; Cook, Peter R.; Murphy, Shona.

In: EMBO Journal, Vol. 17, No. 6, 16.03.1998, p. 1768-1778.

Research output: Contribution to journalArticle

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AU - Cuello, Paula

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AU - Cook, Peter R.

AU - Murphy, Shona

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