Regions of allelic imbalance in the distal portion of chromosome 12q in gastric cancer

B. G. Schneider, SunYoung Rha, Hyuncheol Chung, J. C. Bravo, R. Mera, J. C. Torres, K. T. Plaisance, R. Schlegel, C. M. McBride, X. T. Reveles, R. J. Leach

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Abstract

Aims: To define regions of loss on the distal portion of chromosome 12q in gastric adenocarcinoma. Methods: Microsatellite analysis on chromosome 12 was performed on 19 human gastric cancer cell lines using 77 markers, 71 of which were within or distal to 12q21; some portions of this region showed extended regions of homozygosity (ERHs) in 10 of 19 gastric cancer cell lines. In addition, microdissected tumour cells from 76 primary gastric adenocarcinomas were examined using 13 markers of interest implicated by the cell line data; 70% of these showed allelic imbalance (Al) at one or more markers in or distal to 12q21. Results: Mapping ERHs in the cell lines and sites of Al in the tumours identified three regions that contain putative tumour suppressor genes: region A is located within 2.8 Mb between markers D12S1667 and D12S88; region B, within 1.9 Mb between markers D12S1607 and D12S78; and region C, in 0.74 Mb between markers D12S342 and D12S324. Fluorescence in situ hybridisation (FISH) analysis in two cell lines confirmed that two of the ERHs reflected deletions, not amplifications, of D12S81 in region A and D12S340 in region C. FISH analysis of marker D12S1075 within an ERH containing region B in one cell line showed neither amplification nor deletion. Al on 12q was not associated with prognosis, but was associated with ethnicity of the patient. Conclusions: These results identify regions on chromosome 12 that appear to contain tumour suppressor genes important in the development of gastric cancer.

Original languageEnglish
Pages (from-to)141-149
Number of pages9
JournalJournal of Clinical Pathology - Molecular Pathology
Volume56
Issue number3
Publication statusPublished - 2003 Jun 1

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Allelic Imbalance
Stomach Neoplasms
Chromosomes
Cell Line
Chromosomes, Human, Pair 12
Tumor Suppressor Genes
Fluorescence In Situ Hybridization
Stomach
Adenocarcinoma
Microsatellite Repeats
Neoplasms

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Schneider, B. G. ; Rha, SunYoung ; Chung, Hyuncheol ; Bravo, J. C. ; Mera, R. ; Torres, J. C. ; Plaisance, K. T. ; Schlegel, R. ; McBride, C. M. ; Reveles, X. T. ; Leach, R. J. / Regions of allelic imbalance in the distal portion of chromosome 12q in gastric cancer. In: Journal of Clinical Pathology - Molecular Pathology. 2003 ; Vol. 56, No. 3. pp. 141-149.
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title = "Regions of allelic imbalance in the distal portion of chromosome 12q in gastric cancer",
abstract = "Aims: To define regions of loss on the distal portion of chromosome 12q in gastric adenocarcinoma. Methods: Microsatellite analysis on chromosome 12 was performed on 19 human gastric cancer cell lines using 77 markers, 71 of which were within or distal to 12q21; some portions of this region showed extended regions of homozygosity (ERHs) in 10 of 19 gastric cancer cell lines. In addition, microdissected tumour cells from 76 primary gastric adenocarcinomas were examined using 13 markers of interest implicated by the cell line data; 70{\%} of these showed allelic imbalance (Al) at one or more markers in or distal to 12q21. Results: Mapping ERHs in the cell lines and sites of Al in the tumours identified three regions that contain putative tumour suppressor genes: region A is located within 2.8 Mb between markers D12S1667 and D12S88; region B, within 1.9 Mb between markers D12S1607 and D12S78; and region C, in 0.74 Mb between markers D12S342 and D12S324. Fluorescence in situ hybridisation (FISH) analysis in two cell lines confirmed that two of the ERHs reflected deletions, not amplifications, of D12S81 in region A and D12S340 in region C. FISH analysis of marker D12S1075 within an ERH containing region B in one cell line showed neither amplification nor deletion. Al on 12q was not associated with prognosis, but was associated with ethnicity of the patient. Conclusions: These results identify regions on chromosome 12 that appear to contain tumour suppressor genes important in the development of gastric cancer.",
author = "Schneider, {B. G.} and SunYoung Rha and Hyuncheol Chung and Bravo, {J. C.} and R. Mera and Torres, {J. C.} and Plaisance, {K. T.} and R. Schlegel and McBride, {C. M.} and Reveles, {X. T.} and Leach, {R. J.}",
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Schneider, BG, Rha, S, Chung, H, Bravo, JC, Mera, R, Torres, JC, Plaisance, KT, Schlegel, R, McBride, CM, Reveles, XT & Leach, RJ 2003, 'Regions of allelic imbalance in the distal portion of chromosome 12q in gastric cancer', Journal of Clinical Pathology - Molecular Pathology, vol. 56, no. 3, pp. 141-149.

Regions of allelic imbalance in the distal portion of chromosome 12q in gastric cancer. / Schneider, B. G.; Rha, SunYoung; Chung, Hyuncheol; Bravo, J. C.; Mera, R.; Torres, J. C.; Plaisance, K. T.; Schlegel, R.; McBride, C. M.; Reveles, X. T.; Leach, R. J.

In: Journal of Clinical Pathology - Molecular Pathology, Vol. 56, No. 3, 01.06.2003, p. 141-149.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Regions of allelic imbalance in the distal portion of chromosome 12q in gastric cancer

AU - Schneider, B. G.

AU - Rha, SunYoung

AU - Chung, Hyuncheol

AU - Bravo, J. C.

AU - Mera, R.

AU - Torres, J. C.

AU - Plaisance, K. T.

AU - Schlegel, R.

AU - McBride, C. M.

AU - Reveles, X. T.

AU - Leach, R. J.

PY - 2003/6/1

Y1 - 2003/6/1

N2 - Aims: To define regions of loss on the distal portion of chromosome 12q in gastric adenocarcinoma. Methods: Microsatellite analysis on chromosome 12 was performed on 19 human gastric cancer cell lines using 77 markers, 71 of which were within or distal to 12q21; some portions of this region showed extended regions of homozygosity (ERHs) in 10 of 19 gastric cancer cell lines. In addition, microdissected tumour cells from 76 primary gastric adenocarcinomas were examined using 13 markers of interest implicated by the cell line data; 70% of these showed allelic imbalance (Al) at one or more markers in or distal to 12q21. Results: Mapping ERHs in the cell lines and sites of Al in the tumours identified three regions that contain putative tumour suppressor genes: region A is located within 2.8 Mb between markers D12S1667 and D12S88; region B, within 1.9 Mb between markers D12S1607 and D12S78; and region C, in 0.74 Mb between markers D12S342 and D12S324. Fluorescence in situ hybridisation (FISH) analysis in two cell lines confirmed that two of the ERHs reflected deletions, not amplifications, of D12S81 in region A and D12S340 in region C. FISH analysis of marker D12S1075 within an ERH containing region B in one cell line showed neither amplification nor deletion. Al on 12q was not associated with prognosis, but was associated with ethnicity of the patient. Conclusions: These results identify regions on chromosome 12 that appear to contain tumour suppressor genes important in the development of gastric cancer.

AB - Aims: To define regions of loss on the distal portion of chromosome 12q in gastric adenocarcinoma. Methods: Microsatellite analysis on chromosome 12 was performed on 19 human gastric cancer cell lines using 77 markers, 71 of which were within or distal to 12q21; some portions of this region showed extended regions of homozygosity (ERHs) in 10 of 19 gastric cancer cell lines. In addition, microdissected tumour cells from 76 primary gastric adenocarcinomas were examined using 13 markers of interest implicated by the cell line data; 70% of these showed allelic imbalance (Al) at one or more markers in or distal to 12q21. Results: Mapping ERHs in the cell lines and sites of Al in the tumours identified three regions that contain putative tumour suppressor genes: region A is located within 2.8 Mb between markers D12S1667 and D12S88; region B, within 1.9 Mb between markers D12S1607 and D12S78; and region C, in 0.74 Mb between markers D12S342 and D12S324. Fluorescence in situ hybridisation (FISH) analysis in two cell lines confirmed that two of the ERHs reflected deletions, not amplifications, of D12S81 in region A and D12S340 in region C. FISH analysis of marker D12S1075 within an ERH containing region B in one cell line showed neither amplification nor deletion. Al on 12q was not associated with prognosis, but was associated with ethnicity of the patient. Conclusions: These results identify regions on chromosome 12 that appear to contain tumour suppressor genes important in the development of gastric cancer.

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