Abstract
Rhodococcus sp. strain DK17 is able to utilize a variety of monocyclic aromatic hydrocarbons, including benzene, phenol, toluene, and o-xylene, as growth substrates. Although DK17 is unable to grow on m- and p-xylene, this strain could transform these two xylene isomers to some extent after induction by o-xylene. The major accumulating compounds formed during the degradation of m- and p-xylene by DK17 were isolated by high-pressure liquid chromatography and identified by gas chromatography-mass spectrometric and 1H nuclear magnetic resonance spectral techniques. Both xylene isomers were transformed to dihydroxylated compounds by what must be two successive hydroxylation events: m-xylene was converted to 2,4-dimethylresorcinol and p-xylene was converted to 2,5-dimethylhydroquinone. The rigorous structural identification of 2,4-dimethylresorcinol and 2,5-dimethylhydroquinone demonstrates that DK17 can perform distinct regioselective hydroxylations depending on the position of the substituent groups on the aromatic ring.
| Original language | English |
|---|---|
| Pages (from-to) | 211-214 |
| Number of pages | 4 |
| Journal | FEMS Microbiology Letters |
| Volume | 223 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2003 Jun 27 |
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All Science Journal Classification (ASJC) codes
- Microbiology
- Molecular Biology
- Genetics
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Regioselective oxidation of xylene isomers by Rhodococcus sp. strain DK17. / Kim, Dockyu; Kim, Young Soo; Jung, Jae Woo; Zylstra, Gerben J.; Kim, Young Min; Kim, Seong Ki; KIm, Eungbin.
In: FEMS Microbiology Letters, Vol. 223, No. 2, 27.06.2003, p. 211-214.Research output: Contribution to journal › Article
TY - JOUR
T1 - Regioselective oxidation of xylene isomers by Rhodococcus sp. strain DK17
AU - Kim, Dockyu
AU - Kim, Young Soo
AU - Jung, Jae Woo
AU - Zylstra, Gerben J.
AU - Kim, Young Min
AU - Kim, Seong Ki
AU - KIm, Eungbin
PY - 2003/6/27
Y1 - 2003/6/27
N2 - Rhodococcus sp. strain DK17 is able to utilize a variety of monocyclic aromatic hydrocarbons, including benzene, phenol, toluene, and o-xylene, as growth substrates. Although DK17 is unable to grow on m- and p-xylene, this strain could transform these two xylene isomers to some extent after induction by o-xylene. The major accumulating compounds formed during the degradation of m- and p-xylene by DK17 were isolated by high-pressure liquid chromatography and identified by gas chromatography-mass spectrometric and 1H nuclear magnetic resonance spectral techniques. Both xylene isomers were transformed to dihydroxylated compounds by what must be two successive hydroxylation events: m-xylene was converted to 2,4-dimethylresorcinol and p-xylene was converted to 2,5-dimethylhydroquinone. The rigorous structural identification of 2,4-dimethylresorcinol and 2,5-dimethylhydroquinone demonstrates that DK17 can perform distinct regioselective hydroxylations depending on the position of the substituent groups on the aromatic ring.
AB - Rhodococcus sp. strain DK17 is able to utilize a variety of monocyclic aromatic hydrocarbons, including benzene, phenol, toluene, and o-xylene, as growth substrates. Although DK17 is unable to grow on m- and p-xylene, this strain could transform these two xylene isomers to some extent after induction by o-xylene. The major accumulating compounds formed during the degradation of m- and p-xylene by DK17 were isolated by high-pressure liquid chromatography and identified by gas chromatography-mass spectrometric and 1H nuclear magnetic resonance spectral techniques. Both xylene isomers were transformed to dihydroxylated compounds by what must be two successive hydroxylation events: m-xylene was converted to 2,4-dimethylresorcinol and p-xylene was converted to 2,5-dimethylhydroquinone. The rigorous structural identification of 2,4-dimethylresorcinol and 2,5-dimethylhydroquinone demonstrates that DK17 can perform distinct regioselective hydroxylations depending on the position of the substituent groups on the aromatic ring.
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U2 - 10.1016/S0378-1097(03)00379-3
DO - 10.1016/S0378-1097(03)00379-3
M3 - Article
VL - 223
SP - 211
EP - 214
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 2
ER -