Regioselective oxidation of xylene isomers by Rhodococcus sp. strain DK17

Dockyu Kim, Young Soo Kim, Jae Woo Jung, Gerben J. Zylstra, Young Min Kim, Seong Ki Kim, Eungbin Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Rhodococcus sp. strain DK17 is able to utilize a variety of monocyclic aromatic hydrocarbons, including benzene, phenol, toluene, and o-xylene, as growth substrates. Although DK17 is unable to grow on m- and p-xylene, this strain could transform these two xylene isomers to some extent after induction by o-xylene. The major accumulating compounds formed during the degradation of m- and p-xylene by DK17 were isolated by high-pressure liquid chromatography and identified by gas chromatography-mass spectrometric and 1H nuclear magnetic resonance spectral techniques. Both xylene isomers were transformed to dihydroxylated compounds by what must be two successive hydroxylation events: m-xylene was converted to 2,4-dimethylresorcinol and p-xylene was converted to 2,5-dimethylhydroquinone. The rigorous structural identification of 2,4-dimethylresorcinol and 2,5-dimethylhydroquinone demonstrates that DK17 can perform distinct regioselective hydroxylations depending on the position of the substituent groups on the aromatic ring.

Original languageEnglish
Pages (from-to)211-214
Number of pages4
JournalFEMS Microbiology Letters
Volume223
Issue number2
DOIs
Publication statusPublished - 2003 Jun 27

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Regioselective oxidation of xylene isomers by Rhodococcus sp. strain DK17'. Together they form a unique fingerprint.

  • Cite this